Advances in studies on steroidal alkaloids and their pharmacological activities in genus Veratrum / 中国中药杂志

Genus Veratrum plants contain a diversity of steroidal alkaloids, so far at least 184 steroidal alkaloids attributed to cevanine type(A-1～A-69), veratramine type(B-1～B-21), jervanine type(C-1～C-31), solanidine type(D-1～D-10) and verazine type(E-1～E-53), respectively, have been isolated and identified in the genus Veratrum. Their pharmacological activities mainly focused on decreasing blood pressure, anti-platelet aggregation and anti-thrombosis, anti-inflammatory and analgesic, and antitumor effect. This paper classified and summarized the 184 kind of steroidal alkaloids from the Veratrum plants and their major pharmalogical activities in order to provide the scientific basis for the further development and utilization of active alkaloids.

Steroidal alkaloids ameliorate cell proliferation, oxidative stress, inflammation and histology outcome in vitro and in vivo

Objective:To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-α-induced human RA-FLS MH7A in vitro, and against complete Freund’s adjuvant (CFA)-induced arthritis rats in vivo.Methods:CCK-8 assay was utilized to evaluate the anti-proliferation activity in vitro. In in-vivo study, rats were randomly divided into control group, model group (CFA), steroidal alkaloids (STA) groups (5.0, 2.5 and 1.25 g/kg BW), and sarcovagine D (SD) groups (50, 100 and 200 mg/kg BW), 10 rats for each group. To evaluate the anti-inflammation effect, the histology, biochemical parameters and expression of inflammatory cytokines were detected.Results:Steroidal alkaloids and sarcovagine D showed strong anti-proliferative activity during MH7A cell culture proliferation and downregulated NO levels, and inflammatory cytokines (IL-1ß, IL-6 and PGEConclusions:The results suggest that Sarcococca hookeriana var. digyna has anti-inflammatory effect. Steroidal alkaloids and sarcovagine D has the potential to cure RA.

Steroidal alkaloids ameliorate cell proliferation, oxidative stress, inflammation and histology outcome in vitro and in vivo

Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-α-induced human RA-FLS MH7A in vitro, and against complete Freund's adjuvant (CFA)-induced arthritis rats in vivo. Methods: CCK-8 assay was utilized to evaluate the anti-proliferation activity in vitro. In in-vivo study, rats were randomly divided into control group, model group (CFA), steroidal alkaloids (STA) groups (5.0, 2.5 and 1.25 g/kg BW), and sarcovagine D (SD) groups (50, 100 and 200 mg/kg BW), 10 rats for each group. To evaluate the anti-inflammation effect, the histology, biochemical parameters and expression of inflammatory cytokines were detected. Results: Steroidal alkaloids and sarcovagine D showed strong anti-proliferative activity during MH7A cell culture proliferation and downregulated NO levels, and inflammatory cytokines (IL-1β, IL-6 and PGE

Two new steroidal alkaloids from bulbs of Fritillaria pallidiflora / 中草药

Objective: To investigate the chemical constituents in the bulbs of Fritillaria pallidiflora collected from Xinjiang province, China. Methods: Compounds 1-4 were separated and purified by column chromatographies with silica gel, preparative TLC. Their chemical structures were elucidated on the basis of spectroscopic (MS, NMR, etc) methods. Results: Four compounds, yibeirine (1), yibeisine (2), 2'-methoxyseselin (3), and 1-O-β-D-glucopyranosyl-(2S,3S,4R,8Z)-2-[(2'R)-(2'-hydroxydocosanoyl) amido]-1,3,4-octadecanetriol-8-ene (4), were isolated from cyclohexane fraction of 80% aqueous ethanol extract from the bulbs of F. pallidiflora. Conclusion: Compounds 1 and 2 are new steroidal alkaloids.