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Objective: To investigate the antidepressant mechanism of supercritical CO2 extract from Compound Chaigui Precription from the perspective of cAMP signaling pathway. Methods: The rats model were established by chronic unpredictable mild stress (CUMS). The rats were administrated with high, medium and low doses of supercritical CO2 extract from Compound Chaigui Precription and venlafaxine as intervention drugs. The protein expression levels of cAMP, PKA, p-CREB and BDNF in tissues and their corresponding mRNA levels were explored for their antidepressant mechanism. Results: Compared with the blank group, the expression levels of cAMP, PKA, p-CREB and BDNF in the hippocampus of the model group were significantly decreased (P < 0.05, P < 0.01). After the intervention of the Compound Chaigui Precription, compared with the model group, there was a significant difference in the callus of each dose group. At the same time, the mRNA levels of TrkB, PKA, p-CREB and BDNF in the hippocampus of the model group were significantly decreased (P < 0.05, P < 0.01). After the intervention, there was a callback in each dose group compared with the model group, with significant differences. Conclusion: Supercritical CO2 extract from Compound Chaigui Precription can exert antidepressant effect by regulating cAMP-PKA-CREB-BDNF signaling pathway.
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OBJECTIVE:To observe the protective effect of different extraction samples from Salvia miltiorrhiza decoction on acute myocardial ischemia model mice. METHODS:96 mice were randomized into blank control group (normal saline),model group(normal saline),positive drug group [Compound Salvia miltiorrhiza tablet,4.6 mg(crude drug)/(kg·d)],high-dose,medi-um-dose and low-dose groups of water extract,supercritical CO2 extract and supercritical CO2 extract+dregs water extract [24,12, 6 mg(crude drug)/(kg·d)],with 8 mice in each group,12 groups in total. They were given medicine intragastrically once a day. On sixth day,30 min after giving drugs,those groups were given isoprenaline 20 mg/kg,ih,to induce myocardial ischemia model except for blank control group,once a day,for consecutive 3 d. Electrocardiogram ST segment variation of mice were observed. The times and duration of buccal respiration were observed after rapid decapitation,and the activities of CK and LDH in serum were determined. RESULTS:Compared with blank control group,electrocardiogram ST segment and the activity of CK and LDH increased in model group,while the times and duration of buccal respiration after rapid decapitation reduced(P<0.01);compared with model group,above indicators of medication groups had been improved (P<0.01),and were positively correlated to drug concentration;the indicators of supercritical CO2 extract+dregs water extract group were most close to those of blank control group. CONCLUSIONS:Different extraction samples from Saliva miltiorrhiza decoction all have protective effect on acute myocardial ischemia model mice,and the protective effect of supercritical CO2 extracting+dregs water extracting sample of Salvia Miltiorrhiza decoction is best.
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The study was designed to clarify the antidepressant mechanism of supercritical CO2 extract from Compound Chaigui Fang. We used TCMSP, HIT, Pharm GKB and Gene Cards bioinformatics software to predict and analyze the drug/disease targets and their common targets of compounds in the supercritical CO2 extract of Compound Chaigui Fang for depression. Chronic unpredictable mild stress (CUMS) was used to induce depressed model in rats. Hippocampus and serum were collected after supercritical CO2 extract treatment to detect the potential antidepressant targets with enzyme-linked immunosorbent assay (ELISA). The results predicate that there are 22 chemical compounds and 78 potential therapeutic drug targets for depression. There are 177 disease markers for depression, and 14 common targets for drug intervention of depression, which includes the neurotransmitter transports/metabolic enzyme/receptors, hormone of hypothalamus-pituitary-adrenal/thyroid/gonad axis, immune-associated factor, etc. ELISA results suggest that depression is associated with the low level of phosphate cAMP responsive element-binding protein in hippocampus and the high levels of corticosterone and interleukin 6 in serum with CUMS rat. Those were restored to normal levels by supercritical CO2 extract of Compound Chaigui Fang. The study provides an antidepressant mechanism of supercritical CO2 extract of Compound Chaigui Fang based on the network pharmacology, and a new strategy in the study of the effective extracts of compound Chinese traditional medicine.
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Objective To explain the material foundation of anti-inflammatory and analgesic effects of Jinxuan Zhike Xunxi San. Methods The foot swelling experiments in rat and xylene-induced ear edema experiments in mouse were adopted to observe the anti-inflammatory effects of the material extracted and purified from Jinxuan Zhike Xunxi San. The hot plate-induced pain experiment for studying the analgesic effect was established. Results The material of supercritical CO2 extract, saponins and flavonoids extract and minerals in Jinxuan Zhike Xunxi San had a inhibiting effect on egg-induced foot swelling in rat, saponins and flavonoids extract had a distinct inhibiting effect on xylene-induced ear edema in mouse. Every extract had obvious analgesic effects on hot plate-induced pain and significantly increased the threshold of pain. Conclusion The active ingredient groups of Jinxuan Zhike Xunxi San are the material of supercritical CO2 extract, saponins and flavonoids extract and minerals.