ABSTRACT
0.05 ). CONCLUSION: Shenmai Injection can increase the immunity function.
ABSTRACT
Objective:To make use of the characteristics of presenting and processing tumor antigen of dendritic cell to enhance generation of isotypical but xenogenous T lymphocyte.Methods:Autologous dendritic cells were obtained by culturing peripheral blood monocyte of patients with cervical cancer in the presence of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Dendritic cells were loaded with tumor cell lysate and co-cultured with heterogenous PBMCs from patients to induce generation of tumor specific cytotoxic T cell.The generation rate was measured by MTT.Results:Tumor lysate-loaded DC which was isolated from peripheral blood monocyte of patients with cervical cancer could induce the generation of isotypical but xenogenous T lymphcyte.There was no significant difference between tumor tissue lysate and tumor cell strain lysate(P
ABSTRACT
The role that inositol-1, 1.5 -trisphosphate (IP, ) (?)lays in the proliferation of T lymphocyte induced by IL- 2 was studied. In resting T lymphocytes, IL- 2 did notalter intracellular concentration of IP_3, but Con A stimulation increased IP_3 by 45%. In human IL-2 sensitive T cells, which expressed IL-2 receptors by 83%, the Changes in intracellular IP_3 was dependent upon IL-2 concentrations. The IP_3 increased at IL-2 concentrations of 10 and 50 U/ml and the maximal response of 60% was attained at the concentration of 50 U/ml. At IL-2 concentration of 100 U/ml no increase in IP_3 was observed. After binding of anti-Tac McAb to IL-2 receptors of T lymphocyte the increase in IP_3 at IL-2 concentrations of 10 and 50 U/ml was significantly attenuated. It has been suggested that IP_3 could play the important role in signa l-transduction of IL-2-induced proliferation of T Lymphocyte, which is related to the IL-2 concentrations in T cells culture and the expression of IL-2 receptors on T lymphocyte.