Evaluación in vitro de la fuerza de desprendimiento y de la filtración marginal de coronas provisorias fijadas con diferentes agentes cementantes / In vitro evaluation of retention force and marginal leakage of temporary crowns using different luting agents

Objetivo: Analizar mediante pruebas in vitro la fuerza de desprendimiento y la microfiltración marginal de restauraciones coronarias temporales, realizadas sobre premolares, confeccionadas con tres tipos de resinas acrílicas y cementadas con tres materiales de fijación. Materiales y métodos: Se utilizaron 108 premolares a los que se les tallaron pilares con paredes convergentes hacia oclusal (8°), desgaste de 0,8 mm y chánfer a nivel adamantino. Las muestras fueron distribuidas en dos grupos: 54 muestras, para pruebas de desprendimiento; y 54, para pruebas de filtración. Los materiales utilizados en la confección de las coronas fueron: grupo A, resina de acrílico reticulado con relleno de micropartículas (Sistema Cerec, Sirona), grupo B, resina Duralay (Reliance Dental) y grupo C, resina Luxatemp (DMG). Para el cementado se emplearon: subgrupo I, cemento de hidróxido de calcio (Dycal; Dentsply); subgrupo II, cemento de óxido de zinc con el agregado de fluoruros, clorhexidina y nitrato de potasio (GC Temp Advantage; American In); subgrupo III, cemento de ZnO con el agregado de silicona y silano (Temposil; Coltène). Se desarrolló el estudio in vitro de la fuerza de desprendimiento y se realizó un análisis de correlación entre la fuerza (N) requerida para el desalojo de las coronas y la superficie de unión corona-diente (mm2). El análisis de filtración fue efectuado con lupa estereoscópica Olympus (20X y 32X), posicionando las muestras sobre portaobjeto y accionando luz incidente blanca. Por contraste colorimétrico, se determinó el grado de filtración por la penetración longitudinal del colorante en la interfaz diente-restauración. Resultados: Se encontró correlación significativa entre la fuerza requerida para el desalojo de las coronas y la superficie de unión corona diente (correlación de Pearson: 0,423, P=0,0001). Los valores medios obtenidos de la fuerza de desprendimiento fueron: grupo A, 41,09 N; grupo B, 43,52 N; grupo C, 41,26 N, sin diferencias significativas (P=0,484). Con respecto al factor cemento, subgrupo I, 56,87 N; subgrupo II, 40,75 N; y subgrupo III, 28,25 N, y la diferencia fue estadísticamente significativa (P=0,0001). En relación con las pruebas de filtración marginal in vitro considerando solo el factor categoría corona, se observó que los valores de longitud de filtración estuvieron dentro de rangos similares en los tres grupos, aunque el grupo B registró los valores más altos. Teniendo en cuenta solo el factor cemento, el subgrupo II arrojó los valores más bajos, en tanto que los subgrupos I y III registraron valores semejantes y algo mayores, con una media de 0,94 mm y 1,00 mm, respectivamente. La combinación que arrojó menor penetración fue la del grupo A con el subgrupo II (media de 0,62 mm), en tanto que la combinación con valores de longitud o profundidad de filtración más elevados fue la del grupo B con el subgrupo III (media de 1,25 mm), aunque no se alcanzaron diferencias estadísticamente significativas. Conclusión: Se pudo observar que las coronas cementadas con hidróxido de calcio generan las mejores condiciones referidas a la fuerza de desprendimiento, y que las confeccionadas por el sistema Cerec Sirona, mediante tecnología CAD-CAM y cementadas con cemento de óxido de zinc con el agregado de fluoruros, clorhexidina y nitrato de potasio, podrían ofrecen mejores propiedades en cuanto a la filtración marginal (AU)

Aim: To in-vitro analyze the detachment force and the marginal leakage of bicuspid temporary crowns made with three types of acrylic resins and cemented with three luting agents, Materials and methods: 108 premolars were used to drill 0.8 mm depth preparations with 8° taper and chamfer at adamantine level. Half of the specimens were used for detachment tests and the other for leakage. Temporary crowns were fabricated using: group A, acrylic resin with microparticle fillers (Cerec System, Sirona); group B, Duralay resin (Reliance Dental); and group C, Luxatemp resin (DMG). Dycal calcium hydroxide cement (Dentsply)subgroup I, zinc oxide cement with added fluorides, chlorhexidine and potassium nitrate GC TempAdvantage (American In) subgroup II and zinc oxide cement with silicone aggregate and Temposil Silane (Coltène) subgroup III, were used as luting agents and the load required to detach each of the crowns was determined. A correlation analysis was performed between the force (N) required and the crown-tooth involved area (mm2). Microleakage was assessed using an Olympus stereomicroscope (20X and 32X), under white incident light. By colorimetric contrast, the degree of leakage was defined by the longitudinal penetration of a dye at the tooth-restoration interface. Results: Significant correlation (Pearson: 0.423, P=0.0001) was found between force of detachment and surface. Average values obtained from the detachment force were: group A, 41.09 N; group B, 43.52 N; group C, 41.26 N, without significant differences (P=0.484). The cement factor, subgroup I, 56.87 N; subgroup II 40.75 N; and subgroup III, 28.25 N, was found significant (P=0.0001). Leakage was similar in the three groups, although group B recorded the highest values. Taking into account only the cement factor subgroup II yielded the lowest values, while subgroups I and III recorded similar and somewhat higher values, with an average of 0.94 mm and 1,00 mm, respectively. The combination that showed the lowest penetration was that of group A with subgroup II (average 0.62 mm), while the combination with higher values was seen in group B with subgroup III (average 1.25 mm). Conclusion: Temporary crowns cemented with calcium hydroxide generated the best conditions related to the detachment strength and temporary crowns fabricated using Cerec System with CAD-CAM tecnology, luted with a zinc oxide cement with the addition of fluorides, chlorhexidine and potassium showed, although not significant statistically, better properties in terms of marginal leakage (AU)

The influence of disinfection on dimensional stability of temporary crowns / A influência da desinfecção na estabilidade dimensional de coroas temporárias

Objective: Evaluate the influence of different disinfection methods on the dimensional stability of temporary acrylic resin crowns. Material and Methods: A metallic die with two different marks at the margin was used to prepare the specimens with two different resins (bis-acrylic resin-Structur, acrylic resin-Dencrilay). They were divided into eight groups (n=8), determined according to the disinfection methods (microwave, acetic acid, 1% hypochlorite) and control. The marginal adaptation was recorded using an optical microscope (50X) comparing two different marks on the margin of the crowns with 2 points along the entire circumferential margin before and after disinfection methods. Results: Results were compared statistically by ANOVA and Tukey's test (p≤0.05). The two-way ANOVA showed statistical difference between types of materials. No significant differences were found between disinfection methods and interaction of factors. Conclusion: All procedures affected the marginal adaptation however temporary crowns made by bis-acrylic resin demonstrate higher dimensional stability. (AU)

Objetivo: Avaliar a influência de diferentes métodos de desinfecção na estabilidade dimensional de coroas temporárias de resina acrílica. Material e Métodos: um molde metálico com duas marcas diferentes na margem foi utilizado para preparar os espécimes com duas resinas diferentes (resina bis-acrílica - estrutura, resina acrílica-Dencrilay). Eles foram divididos em oito grupos (n = 8), determinados de acordo com os métodos de desinfecção (microondas, ácido acético, 1% de hipoclorito) e controle. A adaptação marginal foi registrada usando um microscópio óptico (50X) comparando duas marcas diferentes na margem das coroas com 2 pontos ao longo de toda a margem circunferencial antes e após os métodos de desinfecção. Resultados: os resultados foram comparados estatisticamente por ANOVA e teste de Tukey (p≤0,05). A ANOVA de dois fatores mostrou diferença estatística entre os materiais utilizados. Não foram encontradas diferenças significativas entre os métodos de desinfecção e a interação dos fatores. Conclusão: Todos os procedimentos afetaram a adaptação marginal, porém as coroas temporárias feitas em resina bis-acrílica demonstram maior estabilidade dimensional. (AU)

Effect of surface treatment of prefabricated teeth on shear bond strength of orthodontic brackets

ABSTRACT Objective: The aim of this in vitro study was to evaluate and compare the effects of grinding, drilling, sandblasting, and ageing prefabricated teeth (PfT) on the shear bond strength (SBS) of orthodontic brackets, as well as the effects of surface treatments on the adhesive remnant index (ARI). Methods: One-hundred-ninety-two PfT were divided into four groups (n = 48): Group 1, no surface treatment was done; Group 2, grinding was performed with a cylindrical diamond bur; Group 3, two drillings were done with a spherical diamond bur; Group 4, sandblasting was performed with 50-µm aluminum oxide. Before the experiment, half of the samples stayed immersed in distilled water at 37oC for 90 days. Brackets were bonded with Transbond XT and shear strength tests were carried out using a universal testing machine. SBS were compared by surface treatment and by ageing with two-way ANOVA, followed by Tukey's test. ARI scores were compared between surface treatments with Kruskal-Wallis test followed by Dunn's test. Results: Surface treatments on PfT enhanced SBS of brackets (p< 0.01), result not observed with ageing (p= 0.45). Groups II, III, and IV showed higher SBS and greater ARI than the Group 1 (p< 0.05). SBS was greater in the groups 3 and 4 (drilling, sandblasting) than in the Group 2 (grinding) (p< 0.05). SBS and ARI showed a positive correlation (Spearman's R2= 0.57; p< 0.05). Conclusion: Surface treatment on PfT enhanced SBS of brackets, however ageing did not show any relevance. Sandblasting and drilling showed greater SBS than grinding. There was a positive correlation between SBS and ARI.

RESUMO Objetivo: o objetivo desse estudo in vitro foi avaliar e comparar os efeitos de desgastes, perfurações, jateamento e envelhecimento de dentes pré-fabricados (Dpf) na resistência ao cisalhamento (RC) de braquetes ortodônticos, bem como os efeitos dos tratamentos de superfície no índice de adesivo remanescente (ARI). Métodos: 192 Dpf foram divididos em quatro grupos (n = 48): Grupo 1, sem tratamento; Grupo 2, desgaste de sulcos com broca diamantada cilíndrica; Grupo 3, duas perfurações com broca diamantada esférica; e Grupo 4, jateamento com partículas de óxido de alumínio de 50µm. Antes do experimento, metade das amostras ficou imersa em água destilada a 37oC durante 90 dias. Os braquetes foram colados com Transbond XT e os testes de cisalhamento foram realizados com uma máquina universal de ensaios. A RC foi comparada, por tratamento de superfície e por envelhecimento, utilizando-se análise de variância e teste de Tukey. Os escores ARI foram comparados entre os tratamentos de superfície usando o teste Kruskal-Wallis e o teste de Dunn. Resultados: os tratamentos de superfície aumentaram a RC dos braquetes (p < 0,01) e o envelhecimento não aumentou a RC (p = 0,45). Os Grupos 2, 3 e 4 mostraram maior RC e maior ARI do que o Grupo 1 (p < 0,05). A RC foi maior nos Grupos 3 e 4 (perfuração, jateamento) do que no Grupo 2 (desgaste) (p < 0,05). RC e ARI apresentaram correlação positiva (R2 = 0,57; p < 0,05). Conclusão: o tratamento de superfície dos Dpf aumentou a RC dos braquetes; porém, o envelhecimento não foi relevante. O jateamento e a perfuração mostraram maior RC do que o desgaste. Existe uma correlação positiva entre RC e ARI.

Expression of Ki67 and P53 proteins in gingival tissue after wearing four kinds of temporary crowns / 实用口腔医学杂志

Objective:To study the expression of Ki67 and P53 proteins in gingival tissue after wearing four kinds of temporary crowns.Methods:The experiment animal model was established by temporary prosthesis on dogs' teeth using chemical-curing resin,thermal-curing resin,DMG-TEMP crown material and SWIFT composite resin respectively.The immunohistochemistry Envision method was used to measure the expression of Ki67 and P53 proteins in gingival tissue before tooth preparation,after tooth preparation,1-and 2-week,1 month after wearing the crowns.The teeth without any treatment were surved as control.HE stained gingival tissue slices were observed under microscope.Results:The expression of Ki67 and P53 proteins in gingival tissue increased 1 week after wearing the chemical-curing resin crowns or thermal-curing resin crowns(P0.05).No pathological change was observed in all the samples.Conclusion:Chemical-curing resin crowns and thermal-curing resin crowns may increase the expression of Ki67 and P53 proteins in gingival tissue,but DMG-TEMP crowns and SWIFT resin crowns do not.None of the four kinds of temporary crowns may cause abnormal proliferation of gingival epithelia.