ABSTRACT
Background: Gentamicin is an aminoglycoside-class antibiotic that can lead to an increase in ROS and decrease antioxidant reserves that cause destruction of testicular cells that are known to inhibit cell division in testicular germ cells and protein synthesis in the testes as well as affect the production of testosterone that affects the quality of spermatogenesis. Vitamin C as an antioxidant can counteract ROS so that testicular cells can be repaired.Methods: This study was an experimental research with post tests only control group design on 25 male rats, 2-3 aged months, weight 160-240 g. Animals were divided into 5 groups. Negative control group was placed in cage without treatment, positive control given induced gentamicin 5mg/kgbb for the first 10 days, and 3 treatment given induced gentamicin 5mg/kgbb for the first 10 days and at days 11-51 were given vitamin C at a dose of 1.6mg; 2.25mg; 4.5mg. On 52 day, all of wistar rats perform terminated for analyzed the testosterone levels were by ELISA, sperm count was calculated with then haemocytometer observed with digital microscope, morphology of spermatozoa was analyzed by eosin staining and was observed with digital microscope.Results: Results of this study showed that the average of testosteron serum on control negatif group of 3,82ng/ml, control positif 3.73ng/ml, treatment 1 is 9.52ng/ml, treatment 2 is 8.29ng/ml, and traetment 3 is 2.28ng/ml. Sperm count on control negatif 41.68 million/ml, control positif 21.06million/ml, treatment 1 is 52.92 million/ml, treatment 2 is 57.12million/ml, treatment 3 is 80.88 million/ml and sperm morphology on control negative 74.00%, control positif 46.96%, treatment 1 is 58.82%, treatment 2 is 68.68%, and treatment 3 is 78.72%.Conclusions: It is concluded that vitamin C has significant effect on increasing testosteron level and sperm quality in gentamicin-induced wistar rats.
ABSTRACT
PURPOSE: We aimed to evaluate the effect of testosterone replacement therapy (TRT) on cognitive function and depression in men with testosterone deficiency syndrome. MATERIALS AND METHODS: We carried out a prospective, placebo-controlled trial involving 106 men with total testosterone levels 0.05). At baseline, no significant differences between the TRT and control groups were noted regarding serum testosterone or prostate-specific antigen levels, or regarding the scores for aging symptoms (Aging Males' Symptoms scale), erectile function (5-item International Index of Erectile Function questionnaire), cognitive function (Korean Mini-Mental State Examination), and depression (Beck Depression Inventory). At 8 months after intervention total serum testosterone levels and erectile function scores had significantly increased (p<0.05), whereas the scores for aging symptoms and depression had significantly decreased (p<0.05) in the TRT group; no significant improvement in any parameters was noted for the control group. Notably, significant improvement in cognitive function was noted among patients with cognitive impairment at baseline (cognitive function score <25) who received TRT. CONCLUSIONS: TRT may be considered in men with testosterone deficiency syndrome if low testosterone levels are associated with depression or cognitive impairment.
Subject(s)
Humans , Male , Aging , Cognition , Cognition Disorders , Depression , Hypogonadism , Prospective Studies , Prostate-Specific Antigen , TestosteroneABSTRACT
Avaliou-se a influência do grau de bipartição escrotal sobre os parâmetros reprodutivos em caprinos. Foram utilizados 30 animais, distribuídos em três grupos: Grupo I, animais que apresentavam escroto sem bipartição (n =10); Grupo II, animais com bipartição escrotal até 50 por cento do comprimento dos testículos (n=10); e Grupo III, caprinos com bipartição escrotal acima de 50 por cento do comprimento testicular (n=10). Os parâmetros avaliados foram peso corporal, perímetro escrotal, comprimento testicular, características físicas e morfológicas do sêmen, concentração plasmática de testosterona e libido dos animais, comparando caprinos com escroto bipartido e não bipartido. Os animais que apresentavam bipartição escrotal expressaram maior peso corporal e comprimento testicular, quando comparados com os animais do grupo sem escroto bipartido, sendo que estes apresentaram maior número de células espermáticas defeituosas. Conclui-se que a bipartição escrotal influencia positivamente alguns parâmetros biométricos bem como a qualidade do sêmen em caprinos.
The influence of scrotum division on the reproductive parameters in goats was evaluated. Thirty animals were divided into three groups of 10 animals each as follows. Group I: animals with simple scrotum (n=10); Group II: animals with 50 percent of scrotum bipartite (n=10); and Group III: animals with more than 50 percent of scrotum bipartite (n=10). It was evaluated: body weight, scrotum perimeter and the testicular length, the seminal characteristic, plasmatic concentration of testosterone, and the libido of the animals with different degrees of scrotum division. Animals with divided scrotum had higher weight and testicular length when compared with animals from the first group that had a higher percentage of spermatic defects. It is possible to conclude that there was a positive influence of scrotal bipartition on the biometric parameters and the sperm quality in goats.
Subject(s)
Animals , Male , Body Weights and Measures , Scrotum , Semen , SheepABSTRACT
Introduction: In Vietnam, the andrology field has been developed in recent years. Some studies have indicated the reasons for male infertility. Amongst them, the most common reason is abnormal semen. Asthenozoospermia rate was found at a higher ratio in abnormal semen analysis. \r\n', u'Objectives: To identify the characteristics of sperm and concentration of FSH, LH and Testosterone in serum; and the association between the sperm morphology and sperm motility as well as the concentration of FSH, LH and Testosterone in serum of asthenozoospermia men. \r\n', u'Subjects and methods: 90 asthenozoospermia male partners of infertile couples. The semen was analyzed at the Laboratory for Tissue and Embryo Preservation, Hanoi Medical University. The hormone concentration was measured by the Automated Chemiluminescence System ACS 180. \r\n', u'Results: The mean semen volume: 3.0 \xb1 1.1 (ml); sperm density: 104.7 \xb1 86.7 million/ml; rapid motility rate: 5.9 \xb1 6.4 (%); sperm vitality rate: 67.4 \xb1 20.1 (%); normal morphology rate: 14.4 \xb1 6.9 (%). In serum, the concentration of FSH was 5.3 \xb1 2.5 lUlL; LH: 4.1 \xb1 1.8 IU/L. Testosterone: 18.3 \xb1 9.4 nmol/L. Conclusions: Among the studied asthenozoospermia men, normal semen volume was found at 91.11 %; normal sperm vitality parameter: 84.4%; normal morphology parameter: 45.56%; normal level of FSH concentration: 47.7%; normal level of LH concentration: 26.66%; normal level of Testosterone concentration: 78.89%. There was a strong association between the sperm morphology and sperm motility as well as serum Testosterone concentration. \r\n', u'