ABSTRACT
Objective To study the effect of Tongbiding injection on expression of N-methyl-D-aspartate (NMDA)receptor 1 mRNA in the spinal cord of chronic constriction injury(CCI)rats.Methods Thirty male Spragru-Dawley rats were randomly divided into control group(group A),CCI group(group B)and Tongbiding group(group C),ten rat for eah group.The changes in NR1 mRNA expression in the spinal cord was detected by reverse transcription-polymerase chain reaction(RT-PCR)techniques.Results The expression of NR1 mRNA in the spinal cord was minimal in control group,CCI induced significant increase in the expression of NR1 mRNA in the spinal cord and Tongbiding significantly inhibited the increase in NR1 mRNA expression.There was significant difference between CCI and Tongbiding group(P
ABSTRACT
AIM: In this context, the high performance liquid chromatography (HPLC) of Tongbiding Injection was used as fingerprint examples of traditional Chinese medicine to compare the different methods for evaluating the similarity. METHODS: Computing methods for similarity assessments were first compared on the basis of theoretical analysis, and then comprehensive evaluations for performances of each method of similarity assessment were investigated via data simulation and statistics. RESULTS: The results manifested that each approach had its individual properties and limitations. Distance coefficient has better general properties as compared with other methods of assessing similarity. CONCLUSION: In practie, to estimate the similarity between the fingerprints of traditional Chinese medicines, the appropriate methods would be selected for the different problems, and therefore quality of traditional Chinese medicines would be better controlled.