ABSTRACT
Solid state fermentation (SSF) was carried out for the production of Lglutaminase by the fungal strain Trichoderma koningii using sesamum oil cake as the solid substrate. L-glutaminase has received significant attention in recent years owing to its potential applications in medicine as an anticancer agent, as an efficient anti-retroviral agent and as a biosensor. In food industries it is used as a flavor and aroma enhancing agent. The overall maximum yield of L-glutaminase (19.41 U/gds) was achieved with the optimized process parameters of initial moisture content 65%, initial pH of the medium 7.0, supplemented with Dmaltose (1.0% w/v) and malt extract (1.0% w/v), inoculated with 2ml of 6 day old fungal culture and incubated at 33°C for 5 days. Both physico-chemical and nutritional parameters had played a significant role in the production of the enzyme, L-glutaminase. The enzyme production was found to be growth associated with the growth of the fungal culture.
ABSTRACT
ACEI,ACEII and Xyr1 are transcriptional factors that regulate cellulase gene expression in Trichoderma koningii.In vitro experiments have shown that ACEI and Xyr1 bind to the cbh1 promoter fragment(-304 to-18) and regulate the gene transcription.However,whether ACEII binds to this 287bp fragment is still unclear.To further elucidate the regulatory mechanism of ACEII for cellulase,DNA-binding domains of ACEII from T.Koningii were expressed in E.coli.It could not show binding to the cbh1 promoter fragment(-304 to-18) by electrophoresis mobility shift assays,suggesting that it is Xyr1 but not ACEII binds playing an essential role during induction of cbh1 gene transcription.
ABSTRACT
In this paper,300 bacteria strains and 31fungi strain were isolated from Qinghai plateau.The numbers of cellulose degradation organisms in soil is 2.6?10 5/g. A strain Trichoderma koningii No.0143 which produces cellulase was isolated from 11 fungi in the east area in QingHai,its FPA activity was 15u/g. It can be used in enzymatic feed.