A study on role of lipid peroxidation in diabetic patients in Thiruvarur District

Introduction: Insulin affects many sites of mammalian lipid metabolism It stimulates synthesis of fatty acid in liver adipose tissue and in the intestine The insulin has also been reported to increase the cholesterol synthesis The activity of lipoprotein lipase in white adipose is also increased From this point of view, the assessment of various lipid fractions and lipid peroxide in the cases of Diabetes Mellitus may be of some help in the prognosis of patients and in preventing the possibilities of complications or secondary disorders Aim of the study: To study the level of lipid peroxide in IDDM and NIDDM To find out the correlation between Lipid peroxide and Lipid profile in both types of diabetes mellitus Materials and methods: Participants of the study group were selected from the outpatient's population of the Department of Medicine, Government Thiruvarur Medical College, Thiruvarur 100 patients were selected for this study Out of which 50 patients belong to NIDDM and 50 to IDDM group 50 persons served as a healthy control Results: There was a very good positive correlation between MDA and Cholesterol, TGL, LDL, VLDL in both NIDDM and IDDM cases There was a negative correlation between MDA and HDL in NIDDM and IDDM cases Conclusion: In the diabetes mellitus abnormally increased levels of lipid, lipoprotein and lipid peroxides in plasma may be due to the abnormal lipid metabolism The maximum increase in lipid peroxide was found in a group of diabetes mellitus with complication Elevated levels of lipid peroxide in diabetes mellitus may be due to the alteration of function of erythrocytes membrane This inhibits the activity of superoxide dismutase enzyme leading to accumulation of superoxide radicals which cause the maximum lipid peroxidation and tissue damage in diabetes

Effects of Benincasa hispida Seeds Intake on Blood Glucose and Lipid Levels in Streptozotocin Induced Diabetic Rats

This study was designed to examine the effects of diets containing different levels of seeds of Benincasa hispida (wax gourd) on plasma glucose, insulin levels and lipid profiles in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were induced diabetes mellitus by STZ injection (45 mg/kg) into the tail vein and were divide into 4 groups: normal, STZ-control, two experimental groups. Normal and STZ-control groups were fed an AIN-93 diet and 2 experimental groups were fed a modified diet containing 2.5% and 5.0% of wax gourd seed powder for 4 weeks. The weight gain and feed efficiency ratio in 5.0% seed groups were higher than in STZ-control group. A significant lowering effects of plasma glucose levels were observed in the wax gourd seed 2.5% and 5.0 groups. The plasma insulin level did not differ after treatment with wax gourd seed in experimental rats. The intake of 2.5% wax gourd seed did not result in significant change in plasma cholesterol levels compared to diabetic control group. The intake of 2.5% wax gourd seeds had normalized the increased levels of plasma triglyceride and free fatty acids after 4 weeks. In 5% wax gourd seed group, cholesterol, triglyceride and free fatty acid levels were elevated compared to 2.5% wax gourd seed group. The results of the present study indicate that 2.5% wax gourd seed group would be more effective to control the diabetes than 5% wax gourd seed group.

Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate / 重庆医科大学学报

Objective:To study the triglyceride level changes with or without Diammounium Glycyrrhizinate(DIG)and the potential mechanism on alcoholic fatty liver cell model induced in vitro.Methods:MTT assay was used to screen the optimal concentration of ethanol and DIG injection.Ultra-microstructure was observed under transmission electron microscope.Intracellular triglyceride concentration was measured by hol-automatic biochemistry.Expressions of peroxisome proliferator-activated receptor-gamma (PPAR?),sterol regulatory element binding protein-1(SREBP-1)and SREBP cleavage activating protein(SCAP)were determined by immunocyto-chemistry(ICC).Results:1.The screened optimal DIG concentration was 2.273?g/ml.2.Masses of lipid droplets in cytoplasm were observed under transmission electron microscope.3.Hol-automatic biochemistry measured intracellular triglyceride concentration to be(1.84?0.16)mmol/L and(1.05?0.12)mmol/L respectively in Ld30 group and DIG group,and the difference was statistically significant(P