ABSTRACT
Objective: To explore the mechanism of Leigongteng Pian(Tripterygium wilfordii tablets)for the treatment of rheumatoid arthritis(RA)based on chemomics and network pharmacology. Method: UPLC-Q-TOF/HRMS was used to analyze the chemical constituents of Tripterygium wilfordii tablets under the positive and negative ion modes. Thereafter,the ingredient targets of Tripterygium wilfordii tablets were predicted by Swiss Target Prediction and Pharm- Mapper databases,and the targets of RA diseases were obtained by Traditional Chinese Medicine Systems Pharmacology (TCMSP),Therapeutic Target Database(TTD)and Comparative Toxicogenomics Database(CTD). By taking the inter-section of ingredient targets and disease targets,the direct common targets of Tripterygium wilfordii tablets for the treat- ment of rheumatoid arthritis were obtained. Then the GeneMANIA database was used to obtain indirect targets,and the software of Cytoscape 3.7.1 was used to construct the protein-protein interaction(PPI)networks of potential targets,by which the key targets were screened. Enrichment of gene function and pathways of all potential targets were conducted by Gene Ontology(GO)database and Kyoto Encyclopedia of Genes and Genomes(KEGG)database to explore the mecha- nism of Tripterygium wilfordii tablets for the treatment of rheumatoid arthritis. Results: Thirtyone chemical constituents of Tripterygium wilfordii tablets were screened out and sixtysix potential targets were predicted. Twentyseven active com- ponents in the Tripterygium wilfordii tablets might be important components for the treatment of rheumatoid arthritis as in- dicated by the reverse screening. At the same time,five core targets were screened out,which might be involved in the key pathways that actively participate in the intervention and adjustion of rheumatoid arthritis. Conclusion: In this study,we found that the active components in Tripterygium wilfordii tablets could exert important biological effects via multiple targets and pathways,and these targets and pathways are all involved with the inflammation and immune regula- tion of RA.
ABSTRACT
Objective To investigate the clinical efficacy of Tripterygium wilfordii tablets in the treatment of intractable psoriasis.Methods 66 patients with intractable psoriasis according to the time of admission were randomly divided into two groups.The treatment group was treated with Tripterygium wilfordii tablets,the control group was given erythromycin combined with Glycyrrhizin.During treatment period,the blood and urine were checked every two weeks,the liver and kidney function were examined monthly.After treatment for 6 weeks,the clinical efficacy and side effects were observed in two groups.The patients were followed up for 2 years,the recurrence was observed.Results In the treatment group,8 cases were cured,effective in 15 cases,8 cases were improved,2 cases were ineffective,the total effective rate was 69.7％.Those in the control group were 2 cases,7 cases,5 cases,19 cases,the total effective rate was 27.3％,the difference between the two groups was statistically significant (x2 =7.34,P ＜ 0.05).In the treatment group,mild dizziness in 1 case,transaminase increased in 1 case,irregular menstruation in 1 case,accounted for 9.1％.In the control group,3 cases occured mild dizziness,1 case occured anorexia,transaminase increased in 1 case,irregular menstruation in 1 case,accounted for 18.2％.2 weeks after withdrawal of the drug,they all returned to normal.The incidence rate of side effects between the two groups had statistically significant difference (x2 =3.37,P ＜ 0.05).In the treatment group,1 case relapsed within 1 year,2 cases relapsed within 2 years,the total recurrence rate was 9.1％.In the control group,5 cases relapsed within 1 year,7 cases relapsed within 2 years,the recurrence rate was 39.4％,there was significant difference between the two groups (x2 =5.79,P ＜ 0.05).Conclusion Tripterygium wilfordii tablets in the treatment of refractory psoriasis has short response time,good clinical effect,less side effects,and good long-term effect,which is worthy of promotion.