ABSTRACT
OBJECTIVE:To establish HPLC method for determination of the content of paeoniflorin in Weile capsules.M_ ETHODS:Paeoniflorin was separated on Hypersil C 18 column by using acetonitrile-0.1%phosphoric acid(18∶32)as mobile phase and230nm as UV detection wavelength.RESULTS:There was a good linear relationship between the concentration of paeoniflorin and absorption area value in range of0.25?g～2.5?g.The average recovery was97.9%(RSD=1.09%,n=5). CONCLUSION:The method is highly reproducible and accurate.
ABSTRACT
OBJECTIVE:To establish the quality standard of Welie capsule METHO DS :Fritillary bulb,rhizoma corydalis and magnolia bark in the capsule were ident ified with TLC;the contents of berberine hydrochloride was determined with TLC scanning RESULTS:Fritillary bulb,magnolia bark and rhizoma corydalis could be tested out in TLC chromatogram;berberine hydrochloride appeared good linear re lationship in the range of 0 1～1 0?g,r=0 9 991;and the average recovery w as 97 84%,RSD=2 86%(n=5) CONCLUSION:This method can be used to control the quality of Weile capsule