ABSTRACT
Objective To observe the effectiveness and mechanism of Xiaoyao San (Xiaoyao Powder for Soothing Liver and Relieving Depression) in improving depression-like behavior of rats. Methods Male Wistar rats were randomized into normal group, model group, Xiaoyao San (1.9 g·kg-1·d-1) group, and fluoxetine (2 mg·kg-1·d-1) group. The rats were exposed to chronic unpredictable mild stress ( CUMS) to induce rat depression-like behavior. Field test was performed for the observation of effect of Xiaoyao San on rat depression-like behavior, Luminex liquid chip system was applied to detect the serum cytokines, and the amount and size of rat hepatic sinusoidal endothelial window were examined under electron microscope, and hepatic indoleamine 2, 3-dioxygenase ( IDO) and tryptophan 2, 3 -dioxygenaes ( TDO) expression levels were detected by immunohistochemical and Western blot methods. Results Xiaoyao San showed obvious effect on increasing sugar water consumption, the number of crossing the blocks and erection frequency in rats, decreasing serum levels of tumor necrosis factor alpha ( TNF-α) and interleukin 6 ( IL-6) , increasing the amount of hepatic sinusoidal endothelial window, promoting hepatic sinusoidal endothelial vascularization, and reducing TDO and IDO expression ( P<0.05 or P<0.01). Conclusion Xiaoyao San exerts obvious effect on improving rat depression-like behaviors, and the mechanism is probably related with the decrease of inflammatory factors, inhibition of IDO pathway, and improvement of hepatic sinusoidal endothelial function.
ABSTRACT
【Objective】To investigate the effects of Dan Zhi Xiaoyao San(DZXS) and its extracts on hypothalamic corticotropin releasing hormone(CRH) and plasma corticosterone(CORT) in rats with chronic psychological stress.【Methods】Wistar rats were randomized into 6 groups: A(normal control),B(model control),C (extract of DZXS extracted by water extraction and alcohol precipitation,3.608?g/kg),D(extract of DZXS extracted by petroleum ether,0.087?g/kg),E(extract of DZXS extracted by polysaccharide,2.20?g/kg) and F(DZXS 14.4?g/kg).Except group A,the rat models of chronic multi-phase psychological stress were established in other groups by improved Cart method.Groups A and B were given normal saline,and groups C,D and E were given the corresponding drugs according to the experimental design.Hypothalamic CRH and plasma CORT contents were examined to explore the therapeutic mechanism.【Results】Compared with group A,hypothalamic CRH and plasma CORT contents were increased in group B;hypothalamic CRH content was decreased in groups C,E and F(P
ABSTRACT
【Objective】To investigate the antianxietic action of Xiaoyao San(XS)and Dan Zhi Xiaoyao San(DZXS).【Methods】 Wistar rats were randomized into 5 groups: model control group,high-and low-dose DZXS groups(14.4?g/kg and 7.2?g/kg respectively),and high-and low-dose XS groups(21.06?g/kg and 10.53g/kg respectively).Social interaction test and open-field test were carried out to observe the antianxietic action of XS and DZXS.Except that the model group was given normal saline,the rats in other groups were given the corresponding drugs according to the experimental design for 14 successive days.After treatment,the activities within 5 min were monitored by two observers and then the mean value were calculated.【Results】High-and low-dose DZXS prolonged rats social interaction time and increased the times of body erection and dressing their hair,and low-dose XS also increased the times of body erection and dressing their hair(P