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Objective:To observe the effect of Xinfeng Capsule (XFC) on synovial membrane PI3K/AKT/mTOR pathway in adjuvant arthritis (AA) rats.Methods:Rats were randomly divided into normal control group,model control group,methotrexate group,tripterygium glycoside group and XFC group.The expressions of IL-6,IL-10,HIF-1α and VEGF-A were detected by immunohistochemical method,and the expressions of PI3K,AKT1 and p-AKT1 were detected by immunoblotting.Results:Expression of AKT1,mTOR and ES proteins in synovial blood vessels.The expression of IL-6,VEGF,HIF-1α and synovial membrane PI3K,AKT1,p-AKT1,mTOR,ES in the model group were significantly higher than those in the control group The expression of PI3K,p-AKT1,mTOR,ES in synovial membrane decreased,and IL-10 in serum increased in XFC group,and the expression of HIF-1α,IL-6 and VEGF-A were decreased.Conclusion:XFC can improve angiogenesis in AA synovium by regulating PI3K/AKT/mTOR signaling pathway,HIF-1α and ES expression.
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<p><b>OBJECTIVE</b>To determine the effectiveness and safety of Xinfeng Capsules (XFC) for the treatment of rheumatoid arthritis (RA) patients with decreased pulmonary function.</p><p><b>METHODS</b>This was a randomized controlled clinical trial of 80 RA patients. Participants were assigned to the trial group (40 cases) and the control group (40 cases) by block randomization. The trial group was treated with XFC, three pills each time three times daily for 2 months. The control group was treated with tripterygium glycoside (TPT), two pills each time three times daily for 2 months. Both groups were followed up after 2 months. The clinical effects, changes in joint and pulmonary function, and quality of life before and after treatment were observed; safety indices were also evaluated.</p><p><b>RESULTS</b>Pain, swelling, tenderness, and duration of morning stiffness of joints were obviously decreased after treatment in both the trial and the control groups compared with baseline (P<0.01). Compared with before treatment, hand grip strength increased significantly after treatment in the trial group (P=0.0000); pulmonary function parameters such as forced expiratory volume in the first second of expiration/forced vital capacity (FEV1/FVC), 50% of the expiratory flow of forced vital capacity (FEF50), carbon monoxide diffusing capacity (DLco) were increased (P<0.01 or P<0.05); measures of quality of life such as role-physical, body pain, vitality and mental health were also improved after treatment in the trial group (all P<0.05). Joint swelling in the trial group decreased compared with the control group (P=0.0043), while hand grip strength was increased after treatment (P=0.0000). The increase in FEF50, DLco, and the dimensions of quality of life such as vitality and mental health were all significantly greater in the trial group than the control group (P<0.05 or P<0.01).</p><p><b>CONCLUSIONS</b>XFC not only relieved joint pain in RA patients, but also significantly improved the ventilation and diffusion function of the lungs. Therefore, XFC could improve the whole body function and enhance the quality of life of RA patients.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Arthritis, Rheumatoid , Blood , Drug Therapy , Pathology , Blood Sedimentation , C-Reactive Protein , Capsules , Drugs, Chinese Herbal , Therapeutic Uses , Joints , Pathology , Quality of Life , Respiratory Function Tests , Surveys and Questionnaires , Treatment OutcomeABSTRACT
OBJECTIVE:To study the anti-inflammatory effect of Xinfeng capsule on adjuvant arthritis (AA) in rats. METH-ODS:70 SD rats were randomized into a normal control group(isovolumetric normal saline),a model control group(isovolumet-ric normal saline),a positive control TCM group [Qufeng zhitong capsule 0.4 g(crude drug)/kg],a positive control chemical medi-cine group(Leflunomide tablet 2.1 mg/kg)and groups of low,middle and high doses of Xinfeng capsule,which were respectively marked as groups A,B,C,D,E,F and G. All groups of rats except for Group A were given freund’s complete adjuvant intracu-taneous injection to establishe AA models,and from the 12th day after the inflammation was induced,were given the correspond-ing drug,ig,for 28 consecutive days. The degree of toe swelling and arthritis index were determined and calculated for all groups of rats before and after the administration. The contents of interleukin 1β(IL-1β) and tumor necrosis factor α(TNF-α) in serum and synovial membrane of all groups of rats were determined 24 h after the last administration,and pathomorphological changes of their ankles were observed by light microscope. RESULTS:Compared to group A,group B demonstrated higher degree of toe swelling,arthritis index and contents of IL-1β and TNF-α in serum and synovial membrane,with statistical significance(P<0.01). After the administration,the degree of toe swelling and arthritis index of groups C,D,E,F and G reduced,with statistical signifi-cance(P<0.05). Compared to group B,groups C,D,E,F and G showed lower degree of toe swelling,arthritis index and con-tents of IL-1β and TNF-α in serum and synovial membrane,with statistical significance(P<0.01 or P<0.05). Synovial membrane hyperplasia and inflammatory cell infiltration were noted in the ankle tissue of the rats in group B,and such symptoms were not so serious in groups C,D,F and G. CONCLUSIONS:Xinfeng capsule has anti-inflammatory effect for AA rats by a mechanism which may be related to accelerating synoviocyte apoptosis and inhibiting synovial membrane hyperplasia.
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Objective To observe effects of Xinfeng capsule (XFC) on platelet parameters,ultrastructure,p-selectin and therapeutic effect on rheumatoid arthritis (RA) patients in active phase.Methods Platelet parameters,p-selectin and reactive indexes of 60 RA patients in active phase were detected.RA patients were divided into XFC treated group (35 examples) and Zhengqing Fengtongning (ZQF) control group (25 examples) randomly.After a course of treatment,the therapeutic effect and changes of platelet parameters,p-selectin,reactive indexes of two groups were observed.Setting up a normal control (20 examples) and detecting above-mentioned indexes.Observing platelet ultrastructure of two examples with transmission electron microscope of per groups.Results Compared with normal control group,PLT,PCT,MPV,CD62P of RA patients in active phase was obviously increased.Platelet ultrastructure was obviously damaged.The excellence rate of XFC exceeded ZQF control group.After being treated,platelet ultrastructure of the two groups were obviously improved,and XFC group was better than ZQF group.Compared with ZQF group,PLT,PCT,CD62P were obviously decreased in XFC group.PLT,PCT were positively correlated with CD62P,IgG,ESR,CRP,RF,arthrpain,arthrocele.And PLT was positively correlated with arthrtenderness,morning stiffness time.Conclusion PLT and PCT of RA patients in active phase were increased,and have correlation with reactive indexes.It suggests that PLT,PCT can reflect reactiveness of RA as objective indexes.XFC can not only treat inflammation and delay the aggravation of RA patient by improving platelet ultrastructure.
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Objective To observe effects of Xinfeng capsule(XFC)on depressive mood and serum cortisol in patients with rheumatoid arthritis and study its mechanism.Methods 76 RA patients were randomly divided into two groups.The treatment group(n=40)was treated with XFC,and the control group(n=36)with Zhengqing Fengtongning.The targets included clinical effects,symptom and sign,integral of quality of life,self-rating depression scale(SDS)and serum cortisol.Results The effects of XFC were similar to that of Zhengqing Fengtongning in the total effective rate,improving symptoms and sign,active index,etc.XFC was superior to Zhengqing Fengtongning in improving SDS standard score,the symptoms of splenic asthenia and quality of life(P
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Objective:To observe effects of Xinfeng Capsule(XFC)on platelet parameters(platelet count,thrombocytocrit,mean platelet volume,platelet distribution width),p-select,platelet ultrastructure and its therapeutic effect on rheumatoid arthritis(RA)patients in active phase.Methods:To detect platelet parameters,p-select and reactive indexes(ESR,?1-AGP,CRP,RF)of 60 RA patients in active phase.Patients were divided into XFC treated group(35 examples),Zhengqingfengtongning(ZQF)control group(25 examples)according to random digits table.After a course of treatment,to observe therapeutic effect and the changes of platelet parameters,p-select,reactive indexes of two groups.Setting up a normal control(20 examples)and detecting above-mentioned indexes.To observe platelet ultrastructure with transmission electron microscope.Results:1.Compared with normal control group,PLT,PCT,MPV,Ps of RA patients in active phase increased obviously(P0.05).But the excellence rate of XFC exceeded that of control group(P
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Objective:To observe the effects of effects of Xinfeng capsule(XFC) on platelet parameters(platelet count,thrombocytocrit,mean platelet volume,platelet distribution width) and on the serum levels of cytokines(IL-1?,TNF-?,IL-10) in adjuvant arthritis(AA) rats.Methods:40 SD masculine rats were averagely divided into groups of normal control,model control,XFC treatment,methotrexate(MTX) and tripterygium wilfordii polycoride tablet(TPT) alone,with 8 in each.Except for the rats of normal group,the others were intracutaneously injected with 0.1 ml of Freund′s complete adjuvant in the right hindlimb.The changes of platelet parameters (PLT,PCT,PDW,MPV) were observed by Sysmex XT-2000i automatic cytoanalyze and serum cytokines(IL-1?,TNF-?,IL-10)were analyzed with ELISA kit.Results:1.Compared with those of normal control,platelet count,thrombocytocrit,IL-1?、TNF-? of AA rats were obviously increased and IL-10 was obviously decreased in the model groups(P0.05).3.PLT and PCT of the rats were positively correlated with serum contents of IL-1? and TNF-?,as well as voix pedis′swelling and arthritis index(AI).In addition, IL-10 concentration in the serum was negatively correlated with PLT and PCT(P
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Objective:To observe the influence of Xinfeng capsule(XFC) on pulmonary HRCT images and cytokines(TNF-?,IL-10) in blood serum adjuvant arthritis rats.Methods:60 rats were averagely divided into normal control group,model control group,methotrexate(MTX) treated group,tripterygium wilfordii polycoride tablet(TPT) treated group, XFC treated group,12 in each.Except for the rats of normal group,the others were intracutaneously injected with 0.1 ml of Freund’s complete adjuvant in the right hindlimb.After 19 days,different drugs were given to all rats. Pulmonary HRCT images were obtained and the blood samples were reserved for assaying cytokines.Results:In model control group ,the lungs’ outlines weren’t clear and the blood vessels expanded with the edges of them being obscure and unregular. There were high density images shaped as rope and slip in lungs of model control group rats and the pleura near the images were a little thickening. In MTX treated group, there were high density images shaped as slice along pulmonary veins. The veins of the blood vessels were obscure and the edges were unclear. In TPT treated group,there were multiple high density images shaped as tubercle and slice and the boundary were not clear. In XFC treated group, the lungs’ outlines were clear and the veins were well-distributed. High density images shaped as spot were found in part lungs. Compared with normal control group,tumor necrosis factor-?(TNF-?) in blood serum ascended obviously in model control group(P0.05).Conclusion:XFC eliminates swelling by down-regulating the levels of TNF-?, up-regulating the levels of IL-10, restraining the inflammatory reaction.In adittion,cytokines participated in forming pulmanory fibrofication.So the changes of pulmanory HRCT images were also improved at the same time.