ABSTRACT
Objective: To optimize the extraction process for Xinshenning Tablets. Methods: The extracting condition was optimized by Plackett-Burnman, Box-Behnken, and response surface methodology with the extraction rate, geniposide, and spinosin as indexes. Results: The optimal extracting condition was extracting 3 times with 7.5 fold 70% alcohol, 1.5 h for each time. Conclusion: The optimized process condition is simple and feasible. It can be used in the production.
ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of the contents of geniposide and liquiritin in Xinshenning tablets. METHODS:HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water (gradient elution) at flow rate of 1.0 ml/min,the column temperature was 25 ℃,detection wavelength was 238 nm and the vol-ume was 10 μl. RESULTS:The mass concentration range was 16.3-407.9 μg/ml for geniposide(r=0.999 9) and 7.4-185.9 μg/ml for liquiritin(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.63%;average recoveries were 99.05%(RSD=1.28%,n=9)and 98.00%(RSD=1.84%,n=9),respectively. CONCLUSIONS:The established method is accu-rate,sensitive and simple,and can be used for the contents determination of geniposide and liquiritin in Xinshenning tablets.