Determination of prim-o-glucasylcimifugin and 5-o-methylvisammiosode in Yinhua Ganmao Granules by HPLC / 中成药

AIM: To establish a HPLC method of determining prim-o-glucasylcimifugin and 5-o-methyl-visammiosode in Yinhua Ganmao Granules (Flos Lonicerae Japonicae, Radix Saposhnikovial, Fructus Forsythiae, etc.). METHODS: A Kromasil C_(18) column was used. The mobile phase was MeOH-Acetonitrile-H_2O(30∶15∶80). The detection wavelength was 254 nm. RESULTS: The linear range of prim-o-glucasylcimifugin was within 0.157 5-3.780 5 ?g(r=0.999 9), The linear range of 5-o-methylvisammiosode was within 0.164 4-3.945 6 ?g(r=0.999 8). The average recoveries(n=6) were 99.40% and 96.79%, respectively. RSD were 0.6% and 0.8% respectively. CONCLUSION: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.