ABSTRACT
OBJECTIVE:To apply AOTF-near infrared spectroscopy for quick identification of Shuanghuanglian oral liquid and Yinhuang oral liquid.METHODS:Yinhuang oral liquid and Shuanghuanglian oral liquid samples of different batches from different manufacturers were collected for collection of their near infrared spectra.The qualitative analysis models of the two kinds of oral liquid were established respectively using principal component analysis.Meanwhile,the established models were used to analyze and validate each other.RESULTS:The established qualitative analysis models of Shuanghuanglian oral liquid and Yinhuang oral liquid could be applied to validate and identify each other correctly.CONCLUSION:The method was proved to be a simple,rapid new analytical technique with low cost,and it can be used for a quick identification of Shuangh-uanglian oral liquid and Yinhuang oral liquid.
ABSTRACT
Objective: To establish a method of HPLC for determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid (Flos lonicerae, Radix Scutellariae). Methods: LUNA C18(2) column was used. The acetonitrile 0.1% phosphoric acid solution was used as the mobile phase. The flow rate was 1.0mL?min -1 . The detection wavelength was set as 318nm. Results: For chlorogenic acid, the linear range was 0.2568 ～ 2.0544 ?g, and the average recovery rate was 97.7% with RSD =0.8%. For baicalin, the linear range was 0.9896 ～7.9168?g, and the average recovery rate was 98.0% with RSD =0.9%. Conclusion: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.