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Objective: To prepare arsenic trioxide (As2 O3) albumin microspheres and to analyze their physicochemical properties and in vitro antitumor activities. Methods: As2 O3 albumin microspheres were prepared by emulsification-heat solidification. Physicochemical and the slow-release properties of the microspheres were identified by the electron microscope, thermal stabilization analysis and dynamic dialysis system in vitro. MTT method was used to evaluate the inhibitory effect of the released fluid on human osteosarcoma cell line U-2 OS cells and human lung adenocarcinoma cell line SPC-A-1 cells. Results: The mean diameter of the microspheres was (156 ± 3.56) μm (ranging 56. 4-256. 7 μm), with the AS2 O3 content being (55.22±11.19)% and t1/2 of release time being 23.34 h. The slowly released fluid showed a significant inhibitory effect on U-2 OS and SPC-A-1 cells. Conclusion: As2 O3 albumin microspheres prepared in the present study have satisfactory physicochemical, slow-releasing, and tumor-inhibition properties.
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0.05 ). The average enhancement rate of LV endocardial border was 96.6 %. The myocardial contrast agent perfusions of left ventricular walls were clearly visualized in 30 patients. CONCLUSION: Clinical application of intravenous left heart contrast echocardiagraphy with perfluoropropane-albumin microsphere is feasibility and effective.
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To prepare bio-degradable albumin microspheres containing carboplatin. METHODS: Bovin serum albumin microspheres containing carboplatin were prepared by using emulsion-direct heat cross linking techniques. RESULTS: The microsphere was yellow, powdery, with a mean diameter of 58.2 μm. The microphere loaded (11.26±0.48)% carboplatin. The trapping efficiency was (84.5±3.6)%. The pattern of drug releasing from the microspheres in vitro fitted to zero order release plot, with an initial burst in the first 2 hours. The releasing rate can be expressed by the follow equation: Q=21.90+8.50 t (d), r=0.995 8. t50=3 d. Stored at 4 ℃ and 37 ℃ respectively for 3 months, the microspheres did not alter much in shape, size and drug content. CONCLUSIONS: This method is simple, reproducible, having high drug trapping efficiency. The microsphere obtained is stable and release drug at a consistant rate in vitro, leading to a prosperity in clinical use.
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0.05 ). After once injection both observers considered the number of clearly recognized endocardial border segments increased significantly. The number evaluated by observers A increased from 2.68 ? 0.95 to 5.99 ? 0.10 while from 2.82 ? 1.03 to 5.99 ? 0.11 by observers B( P 0.05 ). The average contrast enhancement rate of LV endocardial border was 99.7 %. Perfluoropropane-albumin microsphere injection had no significant effection on vital signs such as blood prssure, heart rate and respiration. Electrocardiogram didn′t change markedly and the variance of the laboratory findings like blood and urine routine examination, hepatic and renal function was in normal range. Only one case( 0.33 %) had slight side-effects who suffered from mild nausea and diarrhea, which suggested the clinical safety of this contrast agent. Conclusions Perfluoropropane-albumin microsphere injection could enhance the resolution of LV endocardial borders and make the judgement of regional myocardial movement easier. It has little side-effects and will be appropriate for clinical use.
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OBJECTIVE:To determine the content of C 3 F 8 in human albumin microspheres filling-with C 3 F 8 .METHODS:By Tekmar3000purge and trap concentrator coupled with a Hewlett-Packard5972GC/MSD.RESULTS:C 3 F 8 contents in samples were119.1～433.5?g/ml.CONCLUSION:The method is feasible and suitable for quality control.
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OBJECTIVE:To study the preparation of 5% albumin microspheres by sonication and the possible mechanism of microsphere forming.METHODS:The preparation technic was optimized with orthogonal design.The microsphere diameter was measured with Coulter multisizer and the outward apperance was observed with light and electronic microscope.RESUL_TS:The optimal preparation technic was as follows:5% albumin in NS,ultrasound power at 9th grade and 60 seconds of sonication.CONCLUSION:The albumin microspheres filled with air can be successfully prepared with orthogonal design and can be effectively used in intravenous myocardial contrast echocardiography.
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Objective:To prepare arsenic trioxide(As_2O_3)albumin microspheres and to analyze their physicochemical properties and in vitro antitumor activities.Methods:As_2O_3 albumin microspheres were prepared by emulsification-heat solidification.Physicochemical and the slow-release properties of the microspheres were identified by the electron microscope, thermal stabilization analysis and dynamic dialysis system in vitro.MTT method was used to evaluate the inhibitory effect of the released fluid on human osteosarcoma cell line U-2 OS cells and human lung adenocarcinoma cell line SPC-A-1 cells.Results: The mean diameter of the microspheres was(156?3.56)?m(ranging 56.4-256.7?m),with the As_2O_3 content being (55.22?11.19)% and t_(1?2)of release time being 23.34 h.The slowly released fluid showed a significant inhibitory effect on U- 2 OS and SPC-A-1 cells.Conclusion:As_2O_3 albumin microspheres prepared in the present study have satisfactory physicochemical,slow-releasing,and tumor-inhibition properties.