ABSTRACT
Objective To knock down angiopoietin-1 expression in human gastric cancer cell line BGC-823 and to observe its effect of reversing tumor invasion. Methods siRNA sequence fragments was designed to target angiopoietin-1 and transferred into human gastric cancer cell line BGC-823. RT-PCR was used to assess the transcription level of angio-poietin-1 mRNA, then western blot and immunofluorescence were used to examine the expression level of three invasion-as-sociated proteins include integrinβ1, CD44V6 and Ang-1. Cell adhesion ability was evaluated by cell adhesion assay and cell invation was determined by matrigel and transwell plastic dual-chamber culture system. Results Ang-1 mRNA was knocked down by siRNA showed by RT-PCR. The expression of integrinβ1, CD44V6 and Ang-1 were significantly lower than control group(P<0.05), so did the cellular adhesion and invasion abilities(P<0.05). Conclusion Knocking down angiopoietin-1 by siRNA can reverse invasion of human gastric cancer cell line BGC-823 and may provide new ideas and reference for gene therapy of gastric cancer in the future.
ABSTRACT
Objective: To investigate the relationship between the expressions of hypoxia inducible factor-1α (HIF-1α) and cellular adhesion molecules in human pancreatic adenocarcinoma, and discuss the mechanism for the promoting effects of HIF-1α on cancer invasiveness and metastasis and the clinical significance. Methods: Expressions of HIF-1α, E-cadherin and integrin β1 were detected by immunohistochemistry in 34 cases of radically resected pancreatic cancer tissues and 10 normal pancreatic tissues. The association of the expression of HIF-1α with the expression of E-cadherin and integrin β1 and the relationship between expression of HIF-1α and clinical features were analyzed. Results: There were overexpressions of HIF-1α, abnormal expression of E-cadherin, and high expression of integrin in pancreatic cancer tissues. Expression of HIF-1α negatively correlated with E-cadherin but had no significant relationship with expression of integrin. Expression of HIF-1α correlated with pathological staging and lymph node metastasis. Abnormal expression of E-cadherin was associated with tumor differentiation, pathological staging, and lymph node metastasis. Expression of integrin had no correlation with clinical features. Conclusion: HIF-1α promotes cancer invasiveness and metastasis by regulating abnormal expression of E-cadherin. Immunhistochemistry results indicated that detection of HIF-1α and E-cadherin may be valuable for judging the potential malignancy of pancreatic cancer.
ABSTRACT
Objective To study the expression of the fibronectin (FN) and ? 1 integrin (? 1) in epiretinal membranes(ERM) of eyes with proliferative vitreoretinopathy(PVR). Methods Twenty epiretinal membranes were obtained from eyes undergone vitrectomy for retinal detachment complicated with PVR and observed by immunohistochemical methods. Results Overexpression of FN and ? 1 were observed in 18 and 16 membranes respectively. Conclusion The synergism of FN and ? 1 in their action mignt be one of the important roles in the development of PVR.