ABSTRACT
To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts fromPolygonum odoratum.Methods: Leaves and stems ofPolygonum odoratum were extracted using methanol and their anti-hemolytic activity was assessed using 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride which is known to generate free radical damage on cell membranes of red blood cells. This damage, represented by hemolysis, was measured using spectrophotometry. Antibacterial activity was tested by using a broth microdilution method to find minimal inhibitory concentrations against eight bacterial strains. Anti-cancer activity of the extracts was evaluated against a human promyelocytic leukemic cell line (HL-60) by using MTT assay for cell viability and flow cytometry for apoptosis induction and cell cycle analysis.Results: Both leaf and stem extracts have anti-hemolytic activity. The results showed a significantly increased percentage of inhibition in a concentration-dependent manner. Interestingly, the leaf extract showed anti-hemolytic activity to a greater extent than the stem extract. Antibacterial activity of the extracts, as indicated by their minimal inhibitory concentration, using 12.5, 50, 25, 25 μg/mL, was measured againstStaphylococcus epidermidis, Enterococcus faecium,Enterococcus faecalisand Staphylococcus aureus. The leaf extracts also exhibited anti-cancer activity, demonstrated by significantly decreased cell viability of human promyelocytic cells (HL-60), with an IC50 of (350.00±1.85) μg/mL for 48 h and (38.00±0.92) μg/mL for 72 h. Additionally, HL-60 became apoptotic and accumulated in G1-phase after 48 hours of treatment.Conclusions: The extracts ofPolygonum odoratum exhibit potential anti-hemolytic activity. They also have antibacterial activity by inhibiting growth of Gram-positive bacteria. The leaf extract shows anti-cancer activity against HL-60 to a greater extent than the stem extract, causing decreased viability, increased G1-phase accumulation and apoptosis induction.
ABSTRACT
Objective: To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts from Polygonum odoratum. Methods: Leaves and stems of Polygonum odoratum were extracted using methanol and their anti-hemolytic activity was assessed using 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride which is known to generate free radical damage on cell membranes of red blood cells. This damage, represented by hemolysis, was measured using spectrophotometry. Antibacterial activity was tested by using a broth microdilution method to find minimal inhibitory concentrations against eight bacterial strains. Anti-cancer activity of the extracts was evaluated against a human promyelocytic leukemic cell line (HL-60) by using MTT assay for cell viability and flow cytometry for apoptosis induction and cell cycle analysis. Results: Both leaf and stem extracts have anti-hemolytic activity. The results showed a significantly increased percentage of inhibition in a concentration-dependent manner. Interestingly, the leaf extract showed anti-hemolytic activity to a greater extent than the stem extract. Antibacterial activity of the extracts, as indicated by their minimal inhibitory concentration, using 12.5, 50, 25, 25 μg/mL, was measured against Staphylococcus epidermidis, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The leaf extracts also exhibited anti-cancer activity, demonstrated by significantly decreased cell viability of human promyelocytic cells (HL-60), with an IC
ABSTRACT
This study is focused on the in vitro evaluation of the hemolysis inhibitory activity of aqueous extracts of six plants used in the traditional treatment of sickle cell disease in Benin: Morinda lucida, Uvaria chamae, Lonchocarpus cyanescens, Croton zambesicus, Raphiostylis beninensis and Xylopia aethiopica. AS and SS red blood cells are subjected to hyposmotic impact with decreasing concentrations of NaCl solution. All the aqueous extracts of the six plants showed a better contribution in erythrocyte osmotic resistance from the concentration of 5 mg/mL to 1 mg/mL, except the extract at 5 mg/mL of Raphiostylis beninensis that caused hemolysis of both red blood cells AS and SS. The extract at 1 mg/mL of Raphiostylis beninensis and the extracts at 5mg/mL of Xylopia aethiopica and Croton zambesicus showed a high hemolysis inhibition of red blood cells AS and SS. The extracts of Lonchocarpus cyanescens showed moderate hemolysis inhibition of SS red blood cells while extracts of Uvaria chamae inhibited highly the hemolysis of AS red blood cells. As for the roots of Morinda lucida, only the extract at 5mg/mL highly inhibited the hemolysis of the AS red blood cells. This protocol seems appropriate to work with both AS and SS blood because the comparative effects of each tested extract on hemolysis of AS and SS blood showed a good correlation coefficient of Pearson (1 or -1). All the plants tested in this work showed, at different doses, an in vitro antisickling effect and this explains partially their use in the traditional treatment of sickle cell disease.