ABSTRACT
Toxoplasma gondii is an intracellular protozoan parasite that causes a Th1 cellular immunity. Our previous study showed that T. gondii lysate antigen (TLA) treatment in S180 tumor-bearing mice resulted in tumor reduction by suppressing CD31 expression, a marker of angiogenesis. In the present study, to investigate tumor suppressive effect of TLA under the absence of T lymphocytes, athymic nude mice were compared with euthymic mice in the anti-tumorigenic effect triggered by TLA in CT26 tumors. According to the results, intratumorally injected TLA reduced tumor growth and TIMP-1 level, a metastatic marker, in both euthymic and athymic mice. TLA treatment led to a sharp increase in IL-12 expression in serum cytokine profiling of athymic mice, and increased MyD88 signals in macrophages derived from the bone marrow, implying the activation of innate immunity. The selective induction of IL-12 by TLA treatment had an anti-tumorigenic effect.
Subject(s)
Animals , Antigens, Protozoan/immunology , Disease Models, Animal , Immunity, Innate , Immunotherapy/methods , Interleukin-12/blood , Macrophages/immunology , Mice, Inbred BALB C , Mice, Nude , Myeloid Differentiation Factor 88/analysis , Neoplasms/pathology , Toxoplasma/immunology , Treatment OutcomeABSTRACT
Objective To investigate the mechanism of Kanglaite combined with 5-Fu in treating pancreatic carcinoma through studying the changes of Bcl-2,Bax and vascular endothelial growth factor(VEGF) in pancreatic carcinoma cell and the relationship between apoptosis and these changes.Methods The model of athymic mouse subcutaneous transplantation tumor of human pancreatic carcinoma PC-3 cell was established;the expression of Bcl-2,Bax and VEGF were detected in malignant cells using immunohistochemistry;the apoptosis proportion was detected by adopting flow cytometry;the tumor size was detected.Results Kanglaite combined with 5-Fu could obviously depress the expression of Bcl-2 in protein level in malignant cells;apoptosis proportion had no effect on Bax expression;Kanglaite could obviously depress the expression of VEGF in malignant cells.The apoptosis proportion was higher and protein expression of Bcl-2 was obviously lower in group of Kanglaite combined with 5-Fu than that in Kanglaite group or 5-Fu group.What was more,the protein expression of VEGF and the tumor volum was lowest in group of Kanglaite combined with 5-Fu.Conclusion The effect of Kanglaite united with 5-Fu on treatment of pancreatic cancer is better than that of Kanglaite or 5-Fu alone.