ABSTRACT
Aims: The ethanolic extract of Excoecaria agallocha stem bark was evaluated for antioxidant properties and quantification of bioactive polyphenols by HPLC-DAD. Methodology: ABTS assay, total antioxidant capacity, reducing power, total phenolic and flavonoid contents were used for determining antioxidant activities. Results: In ABTS assay, the extract showed 74.11% inhibition at 10 μg/ml and IC50 of 4.80 μg/ml, which was significant compared to ascorbic acid (12.20 μg/ml). The antioxidant capacity was calculated as 686 mg of ascorbic acid/g of extract. The maximum absorbance for reducing power assay was 0.73 at 250 μg/ml. The total phenolic and flavonoid contents were found to be significant (375.46 mg/g of gallic acid, 22.44 mg/g of quercetin equivalent, respectively). In HPLC assay, catechin hydrate had the highest content (713.91 mg/100 g of dry extract). Conclusion: The obtained results suggest the antioxidant activities with high concentration of catechin hydrate in the extract of Excoecaria agallocha stem bark.
ABSTRACT
Increased lipid peroxidation and reduced glutathione levels in liver of rats fed high sucrose high fat (HSHF) diet were normalized by concomitant administration of (+)-catechin hydrate. Plasma non-enzymatic antioxidants viz. α-tocopherol, ascorbic acid and total thiols decrease were also significantly less in rats administered with (+)-catechin hydrate concomitantly with HSHF diet. Thus the present results indicate that (+)-catechin hydrate has antioxidant activity and is effective in reducing oxidative stress. The study is of clinical importance as oxidative stress is known to be the cause of many clinical manifestations viz. cancer, Parkinson’s disease, atherosclerosis, heart failure, myocardial infarction and many other diseases.