The detection of a novel mutation by screening SCN4A gene in normokalemic periodic paralysis / 中华神经科杂志

Objective To detect a novel mutation in SCN4A gene related to normokalemic periodic paralysis (normoPP) in one Chinese family.Methods Genomic DNA of two patients and their relatives in this family was extracted from peripheral blood leukocytes and amplified by polymerase chain reaction (PCR). All 24 exons of SCN4A gene were screened with denaturing high performance liquid chromatography (DHPLC) technology,and then sequence analysis of those DHPLC chromatograms showing heteroduplex were compared with the unaffected controls.Results Routine laboratory tests were carried on within normal ranges with the exception of an elevated creatine kinase (1126 U/L,normal

High performance liquid chromatography in determination of calycosin-7-O-?D-glucoside and formononetin in Radix astragali / 第二军医大学学报

Objective:To determine the contents of calycosin-7-O-?-D-glucoside and formononetin in Astragalus membranaceus(Fisch.) Bge.by high performance liquid chromatography(HPLC).Methods: The HPLC condition was as follows: column: Hypersil ODS 2 column(4.6 mm?250 mm,5 ?m);mobile phase: A was ACN-MeOH(91,V/V),B was H_2O,with gradient elution;flow speed: 1.0 ml/min;detection: 260 nm;temperature of column: room temperature;injection volume: 20 ?l.Astragalus membranaceus(Fisch.) Bge.was extracted with methanol solution twice,each time 20 min.Results: The theoretical plate numbers of calycosin-7-O-?-D-glucoside and formononetin were 50 134 and 25 258,respectively.The calibration curves were linear within the range of(2.022-101.1) ?g/ml for calycosin-7-O-?D-glucoside and(38.04-1522) ?g/ml for formononetin,with their regression function being Y=58 924X-12 352,(r=)(0.999 9) and Y=9 237X-124 447,r=(0.999 9,) respectively.The intra-day and inter-day precisions(RSD) at low,middle and high injection volume were all less than(2.0%.) The limits of detection were(0.202 2) mg/ml for calycosin-7-O-?-D-glucoside and 1.522 mg/ml for formononetin.The recovery rates were 98.34%(RSD=1.33%,n=3) for calycosin-7-O-?-D-glucoside and 98.84%(RSD=0.12%,n=3) for formononetin.The contents of calycosin-7-O-?-D-glucoside and formononetin in 10 different batch of Astragalus membranaceus(Fisch.) Bge.were determined.Conclusion: HPLC is a simple and reliable method for determining the contents of calycosin-7-O-?-D-glucoside and formononetin in Astragalus membranaceus(Fisch.) Bge.

Study on high-performance liquid chromatographic fingerprints of different germplasms of Flos Carthami / 第二军医大学学报

Objective:To study the high-performance liquid chromatographic(HPLC) fingerprints of different germplasms of Flos Carthami. Methods: HPLC was applied in chromatographic separation of 36 different germplasms of Flos Carthami collected from 11 provinces of China.The chromatography condition was: Agilent Zorbax C_(18) column(4.6 mm?250 mm,5 mm);mobile phase: CAN,0.5% H_3PO_4(from 1090 to 8020);running time: 60 min;flow speed: 1.0 ml/min;detection wavelength: 265 nm;and temperature: 20℃.The data were processed by Chromatographic Fingerprint Station designed by Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University.Different germplasms of Flos Carthami were subjected to cluster analysis and their similarity was also evaluated.Results: The different germplasms of Flos Carthami in this study were grouped into 2 chemical types.Samples from northwest of China were of high similarity;samples from the middle China were of high similarity to those from South China.The similarity of some germplasms of Flos Carthami was significantly different.Conclusion: The chemical components of different germplasms of Flos Carthami are obviously different from each other;selection of germplasm is very important in quality control of Flos Carthami.