ABSTRACT
Background: T2DM is a metabolic disease associated with a group of abnormalities including hyperglycemia, dyslipidemia, hypertension, elevated levels of biochemical and inflammatory markers in circulation. This condition predisposes an individual to a number of adverse consequences which include atherosclerotic cardiovascular disease, neuropathy, nephropathy, and retinopathy. Aim of the study: To compare the various biochemical parameters in T2DM patients and healthy age-matched controls. Materials and methods: The Study included 22 type of II diabetic patients as cases and 22 normal individuals as controls. Fasting blood sugar (FBS) and Postprandial blood sugar (PPBS complete hematological profile, lipid profile, total protein, c-reactive protein and calcium levels were measured in plasma of T2DM and compare with healthy controls. Fasting blood samples were collected into labeled centrifuge tubes, after an 8–12 h overnight fast, from the subjects by venepuncture. The blood samples were centrifuged at 2000rpm for 10 min using a desktop centrifuge and the serum separated and kept in labeled sample bottles at −70°C until further analysis. Results: The results showed higher concentrations of RBC, hemoglobin, HCT, and lymphocytes in healthy controls when compared with T2DM patients and lower concentrations of WBC, platelets, MCV, MCH, neutrophils, monocytes and eosinophils in healthy controls when compared with T2DM patients. Serum lipid profiles in plasma of control and T2DM patients. The results showed high levels of serum lipid profiles including cholesterol, HDL-C, LDL-C and total cholesterol ratio in healthy age-matched controls when compared with T2DM patients. In contrast, the levels of triglycerides were found to be lower in healthy controls when compared with T2DM patients. The results showed that levels of plasma glucose, C-reactive protein, HbA1c were significantly (p<0.05) higher in T2DM Jaiprabu Jayaraj, Pandiamunian Jayabal. Analysis of various biochemical parameters of T2DM patients and healthy controls. IAIM, 2019; 6(7): 1-6. Page 2 patients when compared with those without T2DM (healthy controls). There were also significantly (p<0.01) low levels of total bilirubin, ALT, total protein, albumin, total calcium in plasma of TDM patients when compared with plasma from healthy age-matched controls. Conclusion: The findings in this study support the hypothesis that low-grade systemic inflammation is an underlying factor in the pathogenesis of T2DM and also a common antecedent for both T2DM and CVD. The data from this particular study also provide further evidence that inflammatory markers might provide a method for early detection of CVD risk. These data might have many significant implications for the prevention and treatment of T2DM. Modification of lifestyle habits and management of systemic inflammation should be the major targets for the prevention and treatment of CVD in T2DM patients
ABSTRACT
Current retrospective analysis describes the laboratorial profile of patients hypothetically diagnosed with dengue at a First-Aid Health Unit in Recife, Pernambuco State, Brazil. Results of hemograms were assessed by counting platelets and transaminase dosages, in the first half of 2012. Further, 394 patients (252 females or 64% and 142 males or 34%) were listed, mostly during March and April. Hemograms with platelet counting was undertaken with 210 patients (53.3%) and hemogram plus transaminases dosages with 184 patients (46.7%). Thrombocytopenia, neutropenia and atypical lymphocytes occurred in both genders. Hematocrits were highest in males and transaminases were more altered in females. Patients attended at the health unit with clinical symptoms of classical dengue had a laboratory profile of non-specific exams which is a feature of infection by the dengue virus. Diagnosis could not be confirmed and the need of a fast test in the health unit services was mandatory. The above avoids dengue cases not being notified and treated or overestimated.
Estudo retrospectivo que objetivou descrever o perfil laboratorial dos usuários com hipótese diagnóstica de dengue em um Serviço de Pronto Atendimento (SPA) do Recife (PE). Foram avaliados resultados do hemograma com contagem de plaquetas e dosagem de transaminases, no primeiro semestre de 2012. Registraram-se 394 usuários, sendo 252 do sexo feminino (64%) e 142 do sexo masculino (36%). Hemograma com contagem de plaquetas foi realizado em 210 usuários (53,3%) e hemograma junto com dosagem de transaminases em 184 (46,7%). Trombocitopenia, neutropenia e linfócitos atípicos ocorreram em ambos os sexos. O hematócrito mostrou maior elevação em pacientes do sexo masculino. As transaminases estavam mais alteradas nas mulheres. Os usuários atendidos no SPA com sintomas clínicos de dengue clássica apresentaram perfil laboratorial de exames inespecíficos, característico de infecção pelo vírus da dengue, porém, o diagnóstico não pôde ser confirmado. Isto mostra a necessidade de implantação do teste rápido para dengue nos serviços de pronto atendimento, o que irá evitar que casos de dengue não sejam notificados e tratados, assim como outros sejam superestimados.