ABSTRACT
@# Objective To investigate the protective effects of dulaglutide on Alzheimer’s disease (AD)-like neurodegenerativechangesanditsmechanismthereof. Methods Dulaglutide，anewdrugfordiabetes,wasappliedto observeandexploreAD-likeneurodegenerativechangesinducedbyPI3KinhibitorwortmannininSH-SY5Ycells.MTT assaywasusedtoobservethecellvitalityinducedbydulaglutide,wortmanninandglucagon-likepeptide(GLP-1)1receptor antagonistex9-39.WesternblotassaywasusedtodetectthelevelsofphosphorylatedTau,neurofilaments(NFs)andthe proteinsofPI3K/GSK-3βsignalingpathway.Results Dulaglutideimprovedtheimpairedvitality,increasedthelevelsof phosphorylatedPI3KandGSK-3βanddecreasedphosphorylationlevelsoftauandNFsinducedbywortmannininSHSY5Ycells.Ex9-39antagonizedtheeffectsofDulaglutide. Conclusion DulaglutideprotectsSH-SY5YcellsfromADlikeneurodegenerativechangesthroughimprovingPI3K/GSK-3βinsulinsignalingpathway.