ABSTRACT
Objective:To observe the effect of hemp seed oil on the blood lipid and its hepatoprotective efficacy in hyperlipidemic rats. Methods:High fat diet was used to make the hyperlipidemia model in SD rats,and the rats were randomly divided into 6 groups:the normal control group,high fat model group,high dose group(1. 5 g·kg-1 ),medium dose group(1. 0 g·kg-1 ),low dose group (0. 5 g·kg-1 )and Xuezhikang group(0. 5 g·kg-1 ). After 35-day treatment,serum TC,TG,HDL-C,LDL-C,SOD,MDA,ALT, AST and liver SOD were detected. Results:Compared with the model group,hemp seed oil at different dosages could significantly de-crease the contents of serum TC,TG,ALT and the atherogenic index(AI)( P<0. 05 or P<0. 01),and significantly increase the SOD activity in serum and liver(P<0. 01). The contents of serum MDA and AST in the medium and high dose groups were signifi-cantly decreased(P<0. 01),and the content of LDL-C in serum and the content of MDA in liver in the high dose group were signifi-cantly reduced(P<0. 01). Conclusion:Hemp seed oil shows promising blood lipid reducing ability and liver protective effect in hy-perlipidemic rats,and the mechanism may be related with liver lipid metabolism improvement and antioxidant function enhancement.
ABSTRACT
Object To develop the analysis method to determine the content of cannabidiol in the hemp seed oil by HPLC. Methods The chromatographic condition was Irregular-H-C 18 column (250 mm? 4.6 mm, 10 ?m). A mixture of methanol-acetonitrile-water-acetic acid (25∶50∶25∶0.4) was used as the mobile phase with a flow rate of 0.8 mL/min and the detection wavelength was 220 nm at room temperature. Results The calibration curve for cannabidiol showed good linear correlation within the concentration range of 1.2 — 9.6 ?g/mL (r=0.999 4). The average recovery and RSD was 94.6% and 1.9% (n=9) respectively. Conclusion The method is convenient, reliable and with good reappearance.