ABSTRACT
Objective To improve the quality standard of Baoyun pill. Methods The natures of Psoralea corylifolia, Radix Dipsaci and Loranthus parasiticus were detected by thin layer chromatography (TLC); the content of Hesperidine were measured by high performance liquid chromatography (HPLC). The operating conditions by HPLC were InertSustain C18 (4.6 mm×250 mm, 5 μm ), the mixture of methanol-acetic acid-water (35:4:61) as mobile phase, 1.0 ml/min as flow speed and wavelength 283 nm. Results The results showed that the TLC spots of Psoralea corylifolia, Radix Dipsaci and Loranthus parasiticus were fairly clear, and the according blank test showed no interference. Hesperidine showed a good linear relationship within the range of 0.820-4.920 μg(r=1.000 0)with an average recovery of 99.85%(RSD=0.21%).Conclusions The TLC and HPLC are handy,safe and accurate for Baoyun pills to determine the quality.
ABSTRACT
Objective: To establish a method for the simultaneous determination of paeoniflorin, hesperidine, baicalin and chry-sophanol in Xiaoshi Lidan capsules by dual wavelength HPLC. Methods:An Inertsil ODS-3 C18 column (150 mm × 4. 6 mm,5 μm) was used. The mobile phase was acetonitrile-0. 2% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the de-tection wavelength was 230nm for paeoniflorin, hesperidine and baicalin, and 280nm for hrysophanol, and the column temperature was 40℃. Results:The linear range of paeoniflorin, hesperidine,baicalin and chrysophanol was 0. 030-0. 592 μg(r=0. 999 9), 0. 063-1. 264 μg(r=1. 000 0), 0. 205-4. 094 μg(r=1. 000 0) and 0. 008 2-0. 164 μg(r=1. 000 0), respectively. The average recovery of the four components was 99.49% (RSD =0.85%), 99.74%(RSD =0.71%), 99.75%(RSD =0.47%) and 99.08%(RSD =1. 28%), respectively. Conclusion:The method is simple and accurate, which can be used for the content determination of paeoniflor-in,hesperidine,baicalin and chrysophanol in Xiaoshi Lidan capsules.
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Objective To establish a method for the determination of hesperidine inErchen mixture. Methods HPLC method was used with Fortis C18(250 mm×4.6 mm,5μm)column and the mobile phase consisted of acetonitrile-acetic acid-water(22∶4∶74). The flow rate was 1.0 ml/min,the column temperature was 30℃, and the UV detector was set at 283 nm.Results The linear ranges of hesperidine was 2.86-57.20μg(r=0.999 8). The average recovery of Aloin was 99.4%.Conclusion The method is rapid, reliable, simple, repeatable and with high specificity. It can be an important supplement for Erchen mixture elevated standard.
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Objective To extract the flavomids from orange peel and determine the content of total flavmoids in orange peel. Methods The total flavonoids in orange peel were determined by using hesperidine as standard sample and edible alcohol as extraction solvent. Results The total content of flavonids is 19.44 g/kg. Conclusion The method is simple and reproducible. It provides a scientific basis for using orange peel.
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AIM: To develop an HPLC method for determining narigin,hesperidin and neohesperidin in Weili Tablet(Rhizoma Pinelliae,Radix et Rhizoma Gentianae,Fructus Aurantii immaturus etc. ). METHODS: The analysis was carried out with a Diamonsil C18(4. 6 mm ? 250 mm,5 ?m)column and the mobile phase of acetoni-trile-1% glacical acetic acid solution(19:81,v/v). The flow rate was 1. 0 mL/min and column temperature was 30 ℃. The detection wavelength was set at 283 nm. RESULTS: The calibration curve was linear within the range of 6. 0-60. 0 ?g/mL,2. 24-22. 4 ?g/mL and 10. 2-102. 0 ?g/mL for narigin,hesperidin and neohesperidin,respec-tively. The average recoveries were 100. 2% ,98. 8% and 97. 1% for narigin,hesperidine and neohesperidine,re-spectively. CONCLUSION: The method is reproducible,sensitive and accurate for determination of narigin,hes-peridin and neohesperidin in Weili Tablet.