ABSTRACT
Objective To research the effect and clinical significance of integrinα9β1 on the corneal lymphangiogenesis and expression of vascular endothelial growth factor C (VEGF-C) after corneal suture. Methods 80 BALB/c mice were divided into 4 groups, namely normal control group, suture control group, α9β1 group, α9β1 suppression group, with 20 mice in each group. Normal control group received no treatment. Corneal suture was performed in the suture control group, α9β1 group, α9β1 suppression group, and they were treated with levofloxacin, levofloxacin+α9β1, levofloxacin + anti-α9β1 monoclonal antibody (Y9A2), twice every day for 14 days, respectively. A random sample was taken from 2 mice in each group on 3rd, 5th, 7th, 14th, 21st day after suture. Corneal lymphangiogenesis was examined by immunofluorescence staining and immunohistochemical method. RT-PCR, Western blotting were used to detect the expression of VEGF-C. Results Normal cornea showed no lymphatic vessels, but new lymphatic vessels were found in corneal limbus of three suture groups, and lymphatic vessels count (LVC) reached peak value on the 14th postoperative day. New lymphatic vessels were found in corneal limbus of suture control group on the 3rd postoperative day, and the LVC was 3.27±0.25 on the 14th day. Compared with suture control group, the LVC ofα9β1 group (4.93±0.38) was obviously higher on 14th postoperative day (P < 0.05), and the LVC ofα9β1 suppression group (2.25±0.34) was noticeably lower than that in suture control group on 14th postoperative day (P < 0.05). Little VEGF-C expressed in normal cornea, and the expression of VEGF-C was higher in suture control group than in normal control group, reaching the peak level on 14th day (P < 0.05). Compared with suture control group, the expression of VEGF-C increased significantly inα9β1 group, while it decreased obviously inα9β1 suppression group (P < 0.05). Conclusion Integrinα9β1 can promote the growth of new lymphatic vessels, and suppression ofα9β1 can down-regulate the expression of VEGF-C and reduce cornea lymphangiogenesis.
ABSTRACT
Objective To explore the effects of colon carcinoma cells on stimulating canalization of human lymphatic endothelial cells(hLECs)in vitro.Methods hLECs in experiment group were cultured with the supernatant of colon carcinoma cell SW480,and they were cultured in endothelial culture medium in control group.The difference of the 2 groups in the ability of canalization was observed,the changes in cytoskeleton and the expression of Prox1 were detected by immunofluorescence assay,and the expression of integrin ?9 was determined by Western blotting.Results In comparison to the control group,hLECs in experiment group showed stronger ability of canalization,as a copious net-structure appeared on day 7 of cultivation,and the typical tube-structure formed finally on day 14.The number of tube-structure,including lymphatic branches,were greater in hLECs of experiment group(2.93?0.56)than control group(1.56?0.26)from day 6 on(P