ABSTRACT
OBJECTIVE： To identify chemical components of Forsythia suspense. METHODS： UPLC-Q-TOF-MS technology was used for the chemical components analysis of F. suspense. The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1％ formic acid aqueous solution （A）- 0.1％ formic acid acetonitrile solution （B） with gradient elution， at the flow rate of 0.3 mL/min； the column temperature was set at 30 ℃； the sample size was 5 μL. Positive and negative ions were detected by electrospray ionization. The temperature of the ion source was 550 ℃； the atomizing gas was N2； the atomizing gas and the auxiliary pressure were 379.2 kPa； the air curtain pressure was 241.3 kPa； the decluster voltage was 80 V/－80 V； the collision energy was 35 eV/－35 eV； the mass scanning range was 80-1 500 Da. Peakview 2.0 software was used to screen the target components by the first-order mass spectrometry， and calculate the high-resolution and accurate molecular weight of each component， compare with the reference spectrum and related literature， or calculate the elemental composition of fragment ions in the second-order mass spectrometry， analyze their decomposition pathways， then infer the structure of compounds. RESULTS & CONCLUSIONS： 45 kinds of compounds were identified from F. suspense，which included 7 phenylethanol glycosides，5 lignans，5 terpenes， 12 flavonoids，7 organic acids，2 phenols，2 quinones，2 glycosides and 3 other components. There were 19 compounds identified for the first time in F. suspense. The study provides a reference for the in-depth study of the pharmacodynamic substance basis of F. suspense and the rapid qualitative and quantitative analysis of the components.
ABSTRACT
The study used use bimolecular marking methods to evaluate the lignans of Magnolia officinalis and M. officinalis var. biloba. First, we compare the chemical constituents between M. officinalis and M. officinalis var.biloba. There were significant differences in concentration of magnolignan I between leaves of these two varieties. Then we further select the p-hydroxyphenyl lignin to mining the key enzyme genes of biosynthesis from Magnolia transcriptome, and screened an encoding cinnamyl alcohol dehydrogease gene as the candidate marker of bimolecular marking methods of Magnolia quality by comparing of the expression level and structure variation in homologous gene between M. officinalis and M. officinalis var.biloba. The established method provides the technical support for bimolecular marking methods of Magnolia quality evaluation.