ABSTRACT
Objective:To analyze the relationship between microRNA-137 gene polymorphisms and ischemic post-stroke depression (PSD). Methods:January, 2017 to January, 2019, the single nucleotide polymorphism (SNP) of rs1625579 in microRNA-137 was genotyped in 250 ischemic PSD patients and 250 healthy controls with SNaPshot. The expression of microRNA-137 was measured with quantitative real-time polymerase chain reaction in the mononuclear cells, and the association of the SNP rs1625579 with microRNA-137 expression was analyzed. Results:The allele T was more in the patients than in the controls (OR = 2.033, 95%CI 1.255 to 3.294, P = 0.003), as well as the genotype TT (OR = 2.139, 95%CI 1.272~3.595, P = 0.004). The microRNA-137 expression was less in the patients than in the controls (t = 28.23, P < 0.001). For the controls, the microRNA-137 expression was also less in those with genotype of TT than with GT and GG (t = 3.764, P < 0.001). Conclusion:The genotype TT of SNP rs1625579 in microRNA-137 is a risk factor of susceptibility to ischemic PSD, which may associate with the decrease of expression of microRNA-137.
ABSTRACT
@# Objective： ：To investigate the expression of miR-137 in cervical cancer tissues and cells, and to explore its effect on proliferation, migration and invasion of cervical cancer cells as well as the mechanisms. Methods： ：Thirty-two pairs of cervical cancer tissues and corresponding para-cancerous tissues that surgically resected at the Department of Gynecology and Obstetrics of Dongguan People's Hospital from January 2017 to March 2018 were collected for this study. In addition, cervical cancer cell lines C33A, HeLa, SiHa and cervical epithelial immortalized cell line H8 were also collected. The expression of miR-137 in cervical cancer tissues and cell lines was detected by RT-PCR. miR-137 mimics and miR-137 NC were respectively transfected into C33Aand HeLa cells, and the effects of miR-137 over-expression on proliferation, migration and invasion of cervical cancer cell lines were observed by CCK-8 and Transwell assay. Luciferase reporter gene assay and WB were used to determine the relationship between miR-137 and Wnt5a in cervical cancer. Wnt5a over-expression vector was constructed, and the effects of simultaneous over-expression of Wnt5a and miR-137 on proliferation, migration and invasion of C33Aand HeLa cells were observed. Results： ：The expression level of miR-137 was significantly down-regulated in cervical cancer tissues and cell lines, as compared to para-cancerous tissues and H8 cells (all P<0.05). The over-expression of miR-137 significantly inhibited cell proliferation, migration and invasion of C33A and HeLa cells (all P<0.05). Moreover, Wnt5a was identified as a target of miR-137 by luciferase reporter gene assay. Furthermore, Wnt5a over-expression, to a certain degree, attenuated the suppressive effects of miR-137 on the proliferation, migration and invasion of C33A and HeLa cells. Conclusion： ：miR-137 can inhibit the proliferation, migration and invasion of cervical cancer cells via targeting Wnt5a, which may be an effective target for the treatment of cervical cancer.
ABSTRACT
Schizophrenia is a chronic devastating psychiatric disorder,which severely damages pub-lic mental health. The pathophysiological mechanism of schizophrenia is complex and the etiology of schizo-phrenia is still unclear. Epidemiological study,twins study and family study show that genetic factors play a substantial part in the process of schizophrenia. Previous research indicates that there is a close association between microRNA-137 and schizophrenia,and that the ablation of microRNA-137 significantly affects clini-cal symptoms and prognosis of patients with schizophrenia. So it highlights the relationship between microR-NA-137 and schizophrenia,which reveals its special role in the etiological mechanism of schizophrenia,and may provides new perspective about the treatment of patients in schizophrenia.