High throughput detection and characterization of red blood cells deformability by combining optical tweezers with microfluidic technique / 生物医学工程学杂志

A high throughput measurement method of human red blood cells (RBCs) deformability combined with optical tweezers technology and the microfluidic chip was proposed to accurately characterize the deformability of RBCs statistically. Firstly, the effective stretching deformation of RBCs was realized by the interaction of photo-trapping force and fluid viscous resistance. Secondly, the characteristic parameters before and after the deformation of the single cell were extracted through the image processing method to obtain the deformation index of area and circumference. Finally, statistical analysis was performed, and the average deformation index parameters (

Single Molecule Method for Molecular Biology

In order to understand biological phenomena accurately, single molecule techniques using a physical research approach to molecular interactions have been developed, and are now widely being used to study complex biological processes. In this review, we discuss some of the single molecule methods which are composed of two major parts: single molecule spectroscopy and manipulation. In particular, we explain how these techniques work and introduce the current research which uses them. Finally, we present the oral biology research using the single molecule methods.

From Single Optical Tweezer to Optical Tweezer Array / 航天医学与医学工程

The paper introduced the recent development of optical tweezers array.The particle manipulation system could be set up based on several methods,such as acousto-optics deflectors(AOD),diffractive optical elements(DOE),spatial light modulators(SLM),interference technique,vertical cavity surface emitting laser(VCSEL),optical fibers and micro-lens arrays as well.The principle,specification and basic structure of each method were introduced and analyzed briefly.Taking the advantage of particle trapping,optical tweezers array was very useful for manipulation of micron size particles,such as cells.Combining with microfluidics and MEMs fabrication,optical tweezer array,the ultraminiature and portable particle sorting system,would be a good choice for space biological research.