Efecto antimicrobiano de la irrigación postquirúrgica con agua ozonificada en el alveolo durante exodoncias simples / Antimicrobial effect of postsurgical irrigation with ozonated water in the socket during simple extractions

El presente estudio se propone evaluar los efectos postquirúrgicos de la irrigación del alveolo con agua ozonificada durante una exodoncia simple en pacientes de la Unidad de Atención Odontológica UNIANDES. Para ello se seleccionaron 30 pacientes a los que se les practicaron dos exodoncias en sesiones diferentes aplicándose agua ozonificada post exodoncia solamente en la primera de ellas, lo que permitió comparar en cada alternativa el comportamiento de la intensidad del dolor, grado de inflamación, infección, y tiempo de cicatrización, de manera que las pruebas estadísticas fueron significativas mostrando asociación lineal entre las variables irrigación del alveolo y dolor; y entre la irrigación del alveolo e inflamación. Asimismo, se observó una cicatrización más rápida en los pacientes tratados con el agua ozonificad en comparación a aquellos que no fueron tratados. Además, ese observó una disminución de la biota bucal, especialmente Lactobacillus spp y Streptococcus mutans al irrigar con agua ozonificada responsable de la inflamación y por ende del dolor de los pacientes. Por otra parte, se aprecia un escaso conocimiento acerca de la utilización de agua ozonificada en el alvéolo por parte de los estudiantes, mientras que los profesionales odontólogos, y a pesar de tener conocimiento de los beneficios del agua azonificada, son poco proclives a su uso(AU)

The present study aims to evaluate the post-surgical effects of alveolar irrigation with ozonated water during a simple extraction in patients of the UNIANDES Dental Care Unit. For this, 30 patients were selected who underwent two extractions in different sessions, applying post-extraction ozonated water only in the first one, which allowed comparing in each alternative the behavior of pain intensity, degree of inflammation, infection, and healing time, so that the statistical tests were significant, showing a linear association between the variables irrigation of the socket and pain; and between alveolar blood supply and inflammation. Likewise, faster healing was observed in patients treated with ozonated water compared to those who were not treated. In addition, he observed a decrease in the oral biota, especially Lactobacillus spp and Streptococcus mutans when irrigating with ozonated water, responsible for the inflammation and therefore the pain of the patients. On the other hand, there is little knowledge about the use of ozonated water in the alveolus by students, while dental professionals, despite being aware of the benefits of zonated water, are not inclined to use it(AU)

Study on the effect of vaginal lavage with ozonated water on normal vaginal microecology and lactobacilli / 中国实用妇科与产科杂志

OBJECTIVE: To explore the changes of normal vaginal micro-ecological environment after vaginal lavage with ozonated water and its effect on lactobacillus.METHODS: From April to October 2016,30 volunteers with normal vaginal microecological examination results were recruited from female workers in Beijing Tsinghua Changgung Hospital and selected to receive vaginal lavage with ozonated water,during non-menstrual periods 5 minutes per day,for 3 consecutive days.The vaginal secretions were taken before the first vaginal ozone lavage,the first week,the second week and the third week after the last lavage,and the microecological examination and the difference in the diversity and abundance of the vaginal microbial community were analyzed after 16 S rRNA high-through put sequencing.RESULTS: Before and after healthy women received ozone water for vaginal lavage,there were no significant differences in the microflora evaluation of vaginal secretions,vaginal microbial community diversity or lactobacillus(|cor. p| <0.2).CONCLUSION: Ozonated water for vaginal lavage does not destroy the normal vaginal microecology and has no significant effect on lactobacilli.

Efficacy of combination of ozonated water with oil for treatment of tinea pedis / 中南大学学报(医学版)

Objective:To evaluate efficacy of combined therapy with ozonated water and oil on patients with tinea pedis.Methods:A total of 60 patients with tinea pedis were divided into 2 groups in a randomized and blinded test.Patients in a control group were treated with naftinfine hydrochloride and ketoconazole cream once a day.Patients in an ozone group were treated with ozonated water bath and then ozonated oil topical application once a day.Patients in the 2 groups were treated for 4 weeks.Clinical and laboratory data were collected for both groups at the end of the 1st week,the 2nd week,and the 4th week.The Pearson chi-square was performed to compare scores of the clinical signs and symptoms (CSS) and the mycological result between the 2 groups.Independent samples T-test was performed to compare the curative effect between the 2 groups.Results:After 4 weeks' treatment,6 patients were positive in the control group determined by mycological examination while 1 patient was positive in the ozone group,with no significant difference between the 2 groups (P＞0.05).Changes in CSS at the end of the 1st week,2nd week,and 4th week were obtained and showed no significant difference between the 2 groups at the 3 different time points (P＞0.05).No side effects were observed.Conclusion:Combination of ozonated water with oil is effective on treatment oftinea pedis and it shows no side effects.

Attenuation rules and germicidal efficacy of ozoneted water / 中南大学学报(医学版)

Objective:To determine initial concentrations of ozonated water under different temperatures,attenuation rules ofozonated water under the room temperature (25 ℃),and to inspect the effects ofozonated water under different concentrations on common microorganisms.Methods:The online test method and the plate cultivation method were employed to check the concentrations and killing rates on common microorganisms of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument.Results:The initial concentrations of ozonated water at 20,25,30,35,and 40 ℃ were 4.38,4.26,3.12,2.76,and 1.31 mg/L,respectively.The ozonated water was rapidly attenuated at first 10 min.The concentration ofozonated water still remained at 1.06 mg/L and 0.37 mg/L at 25 and 30 ℃ after 30 min.The average killing rates for Pseudornonas aeruginosa,Escherichia coli,Staphylococcus aureus,methicillin-resistant Staphylococcus aureus,and Candida albicans in 1.0 mg/L ozonated water for 1 min were 99％,100％,100％,100％,and 100％,respectively.The average killing rates of Escherichia coli,Staphylococcus aureus,methicillin-resistant Staphylococcus aureus,Pseudomonas aeruginosa,and Candida albicans in 0.3 mg/L ozonated water for 1 min were 100％,100％,100％,95％,and 92％,respectively.Conclusion:The initial concentrations of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument decrease with the increase of temperature.Ozonated water under 20-30 ℃ has good sterilization effect on common microorganisms.

Ozone therapy as an adjuvant for endondontic protocols: microbiological – ex vivo study and citotoxicity analyses

ABSTRACT Objectives This study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus using a mono-species biofilm model. Parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. Material and Methods: One hundred and eighty single-root teeth were contaminated with a mono-species biofilm of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus. Groups were formed: Group I – control; Group II – standard protocol; Group III – standard protocol + ozone gas at 40 µg/mL; and Group IV – standard protocol + aqueous ozone at 8 µg/mL. In parallel, human gingival fibroblasts were submitted to the MTT test. Cells were plated, then ozone was applied as follows: Group I (control) – broth medium; Group II – aqueous ozone at 2 µg/mL; Group III – aqueous ozone at 5 µg/mL; and Group IV – aqueous ozone at 8 µg/mL. Data were submitted to the Kruskal Wallis test and Bonferroni post hoc analyses to assess microbiology and cytotoxicity, respectively (p<0.05%). Results The results revealed antimicrobial efficacy by Group IV with no CFU count. The cytotoxicity assay showed Groups III and IV to be the most aggressive, providing a decrease in cell viability at hour 0 from 100% to 77.3% and 68.6%, respectively. Such a decrease in cell viability was reverted, and after 72 hours Groups III and IV provided the greatest increase in cell viability, being statistically different from Groups I and II. Conclusion According to the applied methodology and the limitations of this study, it was possible to conclude that ozone therapy improved the decontamination of the root canal ex vivo. Ozone was toxic to the cells on first contact, but cell viability was recovered. Thus, these findings suggest that ozone might be useful to improve root canal results.

Clinical effect and mechanism of combination of ozone,ozonated water and improved vacuum sealing drainage in healing complex wounds / 重庆医学

Objective To investigate the clinical effect of combination of ozone ,ozonated water and improved Vacuum Sealing Drainage(VSD) in healing complex wound ,and to observe the expression of fibroblast growth factors(FGF) and interleukin-1(IL-1) in its surface .Methods 40 patients with complex extremity trauma were selected and divided into 4 groups randomly .These 4 groups were Ozone+VSD group and Ozonated Water+ VSD group ,VSD group and Control group ,and received ozone combined VSD treatment ,ozonated water combined VSD treatment ,pure VSD treatment ,and traditional treatment .Each group contained 10 patients .Comparison of clinical effect ,bacteria loads and the expression of FGF and IL-1 in wound surface was made in 4 groups . Results The average cure time ,average replacing frequency and infection rate in Control group were (31 .5 ± 6 .6)days ,(15 .4 ± 3 .9) times and 40% respectively .The average cure time ,twice VSD cases and infection rate in VSD group were (19 .2 ± 2 .3)days , 5 cases and 10% respectively .There were no case of infection or received twice VSD treatment in Ozone + VSD group and Ozonated Water+VSD group .The average cure time were (14 .9 ± 2 .7) days and (14 .1 ± 3 .0) days .The Ozone+ VSD group and Ozonated Water+VSD group had best clinical effect ,and the Ozonated Water+ VSD group had least bacteria loads in wound surface (P<0 .05) .The expression of FGF and IL-1 in 4 group had obvious differences ,and it had statistical significance(P<0 .05) .Conclusion Both ozone or ozonated water combined VSD treatment could decrease IL-1 and increase FGF expression ,accelerate recovery time ,inhibit bacterial infection ,especially ozonated water combined VSD treatment had better effect .

Disinfection Efficacy of an Automated Endoscopic Reprocessing System with Ozonated Water / 감염과화학요법

BACKGROUND: The aim of this study is to evaluate the effectiveness of automated ozonated water endoscopic reprocessing system (AORS). MATERIALS AND METHODS: Thirty cases were collected and randomly assigned to 3 groups according to the disinfection methods (Group A, AORS for 5 minutes; Group B, AORS for 10 minutes; Group C, automated disinfection with superoxidized water for 3 minutes 30 seconds). After disinfection was finished, samples were collected from the tip of scopes (Site 1, S1) and rinsing water through biopsy channel (Site 2, S2). Samples were inoculated in blood agar plate for 48 hrs, and then colony count was evaluated. RESULTS: Culture positive rate of S1 was 0% in all three groups. Culture positive rates of S2 were 70% (7/10), 70% (7/10) and 90% (9/10) in group A, group B and group C, respectively. High culture rate group (> or = 1 CFU/ml rinsing water) was 0% (0/10), 30% (3/10) and 70% (7/10) in group A, group B and group C, respectively. Disinfection efficacy between group A and C showed a significant difference in high culture rate (P<0.05). CONCLUSIONS: AORS for 5min was at least equally effective in endoscopic reprocessing compared with the conventional superoxidized water system.

Disinfection Efficacy of an Automated Endoscopic Reprocessing System with Ozonated Water / 감염과화학요법

BACKGROUND: The aim of this study is to evaluate the effectiveness of automated ozonated water endoscopic reprocessing system (AORS). MATERIALS AND METHODS: Thirty cases were collected and randomly assigned to 3 groups according to the disinfection methods (Group A, AORS for 5 minutes; Group B, AORS for 10 minutes; Group C, automated disinfection with superoxidized water for 3 minutes 30 seconds). After disinfection was finished, samples were collected from the tip of scopes (Site 1, S1) and rinsing water through biopsy channel (Site 2, S2). Samples were inoculated in blood agar plate for 48 hrs, and then colony count was evaluated. RESULTS: Culture positive rate of S1 was 0% in all three groups. Culture positive rates of S2 were 70% (7/10), 70% (7/10) and 90% (9/10) in group A, group B and group C, respectively. High culture rate group (> or = 1 CFU/ml rinsing water) was 0% (0/10), 30% (3/10) and 70% (7/10) in group A, group B and group C, respectively. Disinfection efficacy between group A and C showed a significant difference in high culture rate (P<0.05). CONCLUSIONS: AORS for 5min was at least equally effective in endoscopic reprocessing compared with the conventional superoxidized water system.