ABSTRACT
ObjectiveTo investigate the effects of inoculation of potassium-solubilizing bacteria on the physiological characteristics of Paris polyphylla var. yunnanensis. MethodThe effects of different potassium-solubilizing bacteria on leaf area，photosynthetic pigment content， and active component indexes of P. polyphylla var. yunnanensis were investigated by pot planting at room temperature. ResultThe results showed that the inoculation of potassium-solubilizing bacteria could increase the leaf area of P. polyphylla var. yunnanensis. The largest average leaf area was observed in the S3 group，reaching 10.497 cm2，with a maximum increase of 11.0% compared with that of the CK group. The inoculation of potassium-solubilizing bacteria could increase the content of photosynthetic pigments in leaves. Among them，the increase in chlorophyll a content was significant （P<0.05），which was 80.6% higher than that of the CK group. The chlorophyll a/b values in all treatment groups were higher than that of the CK group. The activities of superoxide dismutase（SOD），catalase（CAT） and peroxidase（POD） in the leaves of P. polyphylla var. yunnanensis increased by 88.4%，33.8%， and 30.6%，respectively， as compared with that in the CK group. The inoculation of potassium-solubilizing bacteria could promote the accumulation of osmoregulation substances such as soluble sugar and soluble protein in leaf cells of P. polyphylla var. yunnanensis，with a maximum increase of 55.3% and 70.5% respectively compared with that of the CK group. Meanwhile，it also reduced the content of malondialdehyde（MDA），with a maximum decrease of 34.1% compared with that of the CK group. The results of correlation analysis showed that there were some correlations between different chemical components in P. polyphylla var. yunnanensis leaves. ConclusionThe inoculation of potassium-solubilizing bacteria can effectively promote the growth and development of P. polyphylla var. yunnanensis and improve its survival ability in adversity. It is of practical scientific significance and potential application value for improving the survival rate of P. polyphylla var. yunnanensis by artificial planting.
ABSTRACT
The main physiology and biochemistry metabolism including amino acids composition and gamma-aminobutyric acid (GABA) content of sorghum during 3-day germination period was investigated. Respiratory rate increased during germination. Protease activity, free amino acid and reducing sugar content increased with germination time. While moisture, protein, fat and starch content decreased, significantly. Meanwhile, amino acid composition redistributed and the relative content of essential amino acids increased except for leucine and valine. In addition, some nonessential amino acids content such as proline, glycine and histidine was largely enhanced by germination. GABA content increased about 3 folds after germination. Furthermore, combining tannin (the main antinutritional factor) was converted to be free and solvable. These results greatly suggest that germinated sorghum could be a functional food rich in GABA and other health-promoting nutrients.
O principal metabolismo fisiologico e bioquímico incluindo composição de aminoácidos e teor em ácido gama-aminobutírico (GABA), do sorgo durante o período de germinação 3-dia foram investigados. A frequência respiratória aumentou durante a germinação. A atividade de proteases, aminoácidos livres e redução do teor de açúcar aumentou com o tempo de germinação. Enquanto umidade, proteína, gordura e teor de amido diminuiram, significativamente. Enquanto isso, a composição de aminoácidos redistribuído e o teor relativo de ácidos aminados essenciais aumentaram, exceto para leucina e valina. Além disso, alguns conteúdos não essenciais de aminoácidos, tais como prolina, glicina e histidina foram bem melhorados pela germinação. O conteúdo GABA aumentou cerca de 3 dobras após a germinação. Além disso, a combinação de tanino (o principal factor anti-nutricional) foi convertido para ser livre e solúvel. Estes resultados sugerem fortemente que o sorgo germinado pode ser um alimento funcional rico em GABA e outros nutrientes que promovem a saúde.