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OBJECTIVE: To observe the effect of herbal cake-separated moxibustion on blood lipid-apoprotein levels and the expression of Toll-like receptor 2 (TLR 2), TLR 4 and nuclear factor kappa B(NF-κB) mRNAs in atherosclerotic (AS) vulnerable plaques of hyperglycemia rabbits, so as to explore its mechanism underlying improvement of atherosclerosis. METHODS: Sixty New Zealand rabbits were randomly divided into 5 groups: control, model, direct moxibustion, herbal-cake-separated moxibustion and medication groups(n＝12 rabbits in each group). The AS vulnerable plaque model was established by high-fat forage feeding plus balloon-induced abdominal aorta injury and gene transfection of Ad 5-p 53 recombinant vector. Direct moxibustion or herbal-cake-separated moxibustion was applied to "Juque" (CV 14) and bilateral "Tianshu" (ST 25), "Fenglong" (ST 40), or bilateral "Xinshu" (BL 15), "Pishu" (BL 20) and "Ganshu" (BL 18) for 15－20 min every time. The medication group was treated by feeding Atorvastatin. All the treatments were conducted once daily for 8 weeks. Plasma total cholesterol(TC) and triglyceri-de(TG) contents were detected by enzyme method, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) contents detected by colorimetric determination, and plasma apolipoprotein A(Apo-A) and apolipoprotein B(Apo-B) levels determined by electrophoretic method. The pathological changes of vulnerable plaque and the aortic intima and media thickness were observed under light microscope after H．E. staining. The expression levels of TLR 2, TLR 4 and NF-κB mRNAs in AS plaques were determined by quantitative real-time PCR. RESULTS: After modeling, the levels of plasma TC, TG, LDL and Apo-B in the model group were remarkably increased (P0.05). CONCLUSION: Herbal-cake-separated moxibustion has a positive role in stabilizing AS vulnerable plaque in hyperglycemia rabbits, which may be associated with its effects in regulating blood lipid-apolipoprotein levels and inhibiting the expression of TLR 2, TLR 4 and NF-κB mRNAs in vulnerable plaques.
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Objective To investigate the effects of soybean isoflavone(SI) regulating plasma lipid and improving antioxidant ability in diabetic rats.Methods 120 SD rats were randomly devided into a normal group, a diabetes model group, a SI 40, 80, 160 and 320 mg/kg treated group, with 20 expermental animals in each group. Expect the normal group, the diabetic model was setup by intraperitoneal injecting STZ, and the drugs were given by intraperitoneal injection. At 0, 14, 28, 42, 56 days after experiment, the level of plasma glucose were determined. 8 weeks later, the content of TC, TG, LDL-C, HDL-C, the activity of AST, CK, LDHand the T-AOC in serum were determined; the activity of SOD, GSH-Px and the content of MDA in myocardial tissue were determined; and the histopathological changes of myocardial tissue were observed by HE staining. ResultsCompared with the diabetes model control group, the activity of AST in serum of SI 80, 160 and 320 mg/kg treated groups were significantly decreased(104.25 ± 24.92 U/L, 93.71 ± 22.58 U/L, 88.26 ± 23.80 U/Lvs. 127.65 ± 38.17 U/L;P<0.05,P<0.01), the activity of CK were significantly decreased (887.1 ± 185.4 U/L, 831.9 ± 182.8 U/L, 796.2 ± 165.8 U/Lvs. 973.6 ± 211.4 U/L;P<0.05,P<0.01), the activity of LDH were significantly decreased (954.7 ± 153.5 U/L, 868.7 ± 136.2 U/L, 834.1 ± 146.3 U/Lvs. 1 097.6 ± 184.2 U/L;P<0.05,P<0.01); the activity of SOD in myocardial tissue were significantly increased(9.96 ± 2.05 U/mg, 10.47 ± 2.32 U/mg, 11.06 ± 2.29 U/mgvs. 8.72 ± 1.70 U/mg;P<0.05,P<0.01), the content of MDA in myocardial tissue were significantly decreased(5.98 ± 1.35 nmol/mg, 5.47 ± 1.42 nmol/mg, 5.16 ± 1.53 nmol/mgvs. 6.58 ± 1.54 nmol/mg;P<0.05, P<0.01). The plasma glucose level of SI (160 and 320 mg/kg) treated groups were significantly decreased(15.2 ± 0.9 mmol/L, 14.8 ± 0.8 mmol/Lvs. 18.6 ± 1.2 mmol/L;P<0.05,P<0.01), the content of TC in serum were significantly decreased (2.69 ± 0.85 mmol/L, 2.43 ± 0.76 mmol/Lvs. 3.42 ± 0.81 mmol/L;P<0.05,P<0.01), the content of TG were significantly decreased (1.36 ± 0.40 mmol/L, 1.18 ± 0.23 mmol/Lvs. 1.70 ± 0.53 mmol/L;P<0.05,P<0.01), the content of LDL-C were significantly decreased (1.02 ± 0.26 mmol/L, 0.95 ± 0.28 mmol/Lvs. 1.18 ± 0.27 mmol/L;P<0.05), and the content of HDL-C were significantly increased (0.73 ± 0.20 mmol/L, 0.78 ± 0.22 mmol/Lvs. 0.62 ± 0.14 mmol/L;P<0.05), the T-AOC was significantly increased (10.15 ± 2.76 U/L, 11.29 ± 3.47 U/Lvs. 7.95 ± 2.26 U/L;P<0.05), and the activity of GSH-Px was significantly increased(13.79 ± 2.62 U/mg, 14.21 ± 2.87 U/mgvs. 11.90 ± 2.03 U/mg,P<0.05,P<0.01). The histopathological changes of myocardial tissue in SI 160 and 320 mg/kg treated groups were significantly improved.Conclusions SI could effectively lower the plasma glucose level and the blood lipid, improve antioxidant ability, improve antioxidant ability, reduce the damage of free radical, and inhibit the histopathological changes, which suggesting that SI had protective effects on diabetic rats.
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Objective To investigate the association between rs780094 polymorphism in glucokinase regulatory protein (GCKR) and plasma lipid levels in children and adolescents. Methods 1 026 Chinese children aged 7 to 18 years were recruited,with anthropometric measurements,detection of plasma lipid levels and genotyping of rs780094 performed. Relationships between polymorphism and plasma lipid levels were tested,using multivariate linear regression and logistic regression. Results A-allele of rs780094 in GCKR was associated with increased TC,TG and LDL-C levels(b=0.06 mmol/L,P=0.037;b=0.09 mmol/L,P<0.001;b=0.05 mmol/L,P=0.040) under the additive model adjusted for age,age square and gender. The rs780094 in GCKR was also associated with abnormal levels of TG and LDL-C(OR=1.60,95%CI:1.30-1.97,P<0.001;OR=1.35,95%CI:1.02-1.80,P=0.036). Conclusion The rs780094 in GCKR was associated with plasma lipid levels in children and adolescents while A-allele of rs780094 might serve as genetic factor for the increased plasma lipid levels.
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Objective To investigate the association between rs780094 polymorphism in glucokinase regulatory protein (GCKR) and plasma lipid levels in children and adolescents. Methods 1 026 Chinese children aged 7 to 18 years were recruited,with anthropometric measurements,detection of plasma lipid levels and genotyping of rs780094 performed. Relationships between polymorphism and plasma lipid levels were tested,using multivariate linear regression and logistic regression. Results A-allele of rs780094 in GCKR was associated with increased TC,TG and LDL-C levels(b=0.06 mmol/L,P=0.037;b=0.09 mmol/L,P<0.001;b=0.05 mmol/L,P=0.040) under the additive model adjusted for age,age square and gender. The rs780094 in GCKR was also associated with abnormal levels of TG and LDL-C(OR=1.60,95%CI:1.30-1.97,P<0.001;OR=1.35,95%CI:1.02-1.80,P=0.036). Conclusion The rs780094 in GCKR was associated with plasma lipid levels in children and adolescents while A-allele of rs780094 might serve as genetic factor for the increased plasma lipid levels.
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Type 2 diabetic patients are usually accompanied by dyslipidemia.The cardiovascular residual risk is still high in these patients,even with glycemia,blood pressure,and plasma lipids well controlled.In this review,the relationship of plasma lipids and changes in lipoprotein particles with cardiovascular risk is discussed.
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Objective: To investigate the regulation of the silkworm chrysalis oil on the plasma lipid in hyperlipidemia rats and its mechanism. Methods: The rat model of hyperlipidemia was built by high fat diet and then ig administration of silkworm chrysalis oil for six weeks to observe TQ TC, HDL-C, LDL-C, ApoAI, ApoB, LCAT, LPL, and HL levels in the serum of rat every two weeks, then killed the rats in the sixth week, weighed the liver, and detected the LPL and HL levels in the liver. Results: Compared to the indexes of model group, the blood lipids (TG and TC), lipoprotein (HDL-C and LDL-C), apoprotein (ApoAI and ApoB), LCAT, LPL, and HL levels of the rats in every group treated with silkworm oil were significantly different (P<0.05) in a dose-dependent manner, and the change of the relative enzyme level was earlier than that of the blood lipid and lipoprotein level. Conclusion: The silkworm chrysalis oil could regulate the plasma lipid and lipoprotein levels in the serum of rats by activating apoprotein and correlating lipid-metabolizedenzyme, thereby to treat the hyperlipidemia.
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Objective To investigate the effect of Helicobacter pylori (Hp) infection on plasma lpid and high sensitive C reactive protein (hs-CRP) levels in patients with cerebral infarction, and explore the pathogenesy. Methods Seventy-nine cerebral infarction patients without nearly inflammatory reaction disease were recruited. Hp was detected by breath test,the patients were divided into infection-negative group (15 cases), light infection group (29 cases) and severe infection group(35 cases) according to the results,the last two groups were as infection-positive group. The plasma lipid and hs-CRP levels were exanined.Results Compared with infection-negative group,the total cholesterol,low density lipoprotein,hs-CRP levels increased obviously in infecton-positive group (P ＜ 0.05). The level of hs-CRP in severe infection group was higher than that in light infection group [(10.21 ±4.98) mg/L vs. (5.81 ±4.21) mg/L](P=0.001 ). Conclusions Hp infection may increase vascular inflammatory reaction through lipid metabolic disturbance. The cerebral infarction with higher Hp infection, and with the degree of infection increased, the risk is also increased accordingly.
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Objective To study the relationship of interleukin-18 (IL-18)gene-137G/C polymorphisms in the promoter region with cerebral infarction(CI) and the effect of integrin gene polymorphisms on plasma lipid and lipoprotein levels. Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) were used for the detection of integrin genotypes in 190 patients with CI and 210 healthy controls. The plasma lipid and lipoprotein levels were measured by routine method. Results The distributions of IL-18 gene -137G/C polymorphism was significantly different between CI group and control group(P ＜0. 05＝ ,The relative risk suffered from CI of C allele was 1.624 times of the G allele (OR = 1.624,95% CI: 1.134 ～ 2.324), The level of plasma lipid C allele carriers was significantly higher than no carriers(P ＜0.05＝. Conclusion IL-18 gene-137G/C polymorphism was associated with CI, C allele may be genetic susceptibility gene for CI.
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Objective To investigate the effects of Helicohacter pylori infection on plasma lipid levels. Methods HpIgG was measured by ELISA in both 242 patients with coronary heart disease (CHD) and 88 subjects without CHD, and compared between these two groups. Then 242 patients with CHD were divided into HpIgG positive group and HpIgG negative group ,and total cholesterol (TC) ,triglyceride (TG) ,high density lipaprotein (HDL), low density lipoprotein (LDL), apolipoprotein A (ApoA) , apolipoprotein B (ApoB) were analyzed and compared between these two subgroups. Results The rate of seropositivity for HpIgG in CHD patients was significantly higher than that of controls (53.3 % vs. 38.6 %, P < 0.05), and HDL, ApoA level in HplgG seropasitive group was signif-icantly lower than that of HpIgG seronegative one (P < 0.05). However, there was no difference of TC, TC, LDL and ApoB between these two subgroups (P > 0.05). Conclusion Hp infection may be associated with CHD. It may pro-mote the pathogenesis of CHD through lowering serum HDL-C level.
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PURPOSE: The reticuloendothelial system is composed of sinusoidal capillaries, through which even large protein molecules are freely movable between plasma and interstitial space, including the lymphatic system. Therefore, high-dose intravenous immunoglobulin (IVIG) would cause a redistribution of proteins between two compartments. To investigate this hypothesis, we measured plasma protein and lipid levels in patients with Kawasaki disease before and after high-dose IVIG treatment. METHODS: Thirty four children with Kawasaki disease who had complete responses to high-dose IVIG treatment (1 g/kg/day for two consecutive days), were analyzed. Before and after the administration of IVIG, serum analyses were performed for such parameters as total protein, albumin, gamma-globulins (IgG, IgM, IgA), alpha1-, alpha2-, and beta-globulin fractions, and lipid profiles (total cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride). RESULTS: The levels of gamma-globulins including IgG, IgM, IgA were significantly increased, and IgG was increased by 1,779+/-304 mg/dL after two-dose of IVIG infusion. The levels of albumin, alpha1-, alpha2-, and beta-globulin fractions were significantly decreased by 18 percent, 24 percent, 19 percent and 12 percent, respectively. HDL-cholesterol level was significantly decreased by 20 percent, while LDL-cholesterol and triglyceride levels were significantly increased by 21 percent and 50 percent, respectively. The total cholesterol level was not changed. CONCLUSION: High-dose IVIG treatment decreased the levels of a variety of proteins except immunoglobulins, and the increase of IgG after IVIG treatment was lower than expected. Our results suggest that a part of infused IVIG and plasma proteins, including etiologic proteins for Kawasaki disease, may be distributed to the extravascular compartments. The rapid improvement of symptoms induced by IVIG in Kawasaki disease might be explained by this mode of action of IVIG.
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Child , Humans , Beta-Globulins , Blood Proteins , Capillaries , Cholesterol , gamma-Globulins , Immunoglobulin A , Immunoglobulin G , Immunoglobulin M , Immunoglobulins , Immunoglobulins, Intravenous , Lymphatic System , Mononuclear Phagocyte System , Mucocutaneous Lymph Node Syndrome , Plasma , TriglyceridesABSTRACT
Effects of garlic powder supplementation on blood lipid profile and antioxidant system were investigated in rats with and without swimming exercise. Sprague-Dawley rats of four experimental groups were fed for 4 weeks diets containing 15% beef tallow and 1% cholesterol ;control without garlic and exercise, Go with 2% garlic alone, Ex with exercise alone, GoEx with 2% garlic and exercise. Rats were trained 40 min a day, 5 days a week. Group Ex and GoEx showed significant lowering in body weight gain and fat accumulation. In Go, Ex and GoEx, plasm TG and LDL-C were lower and HDL-C was higher, although not significantly, compared to levels in control. Total cholesterol was significantly reduced in group Go, and Ex and GoEx were lower than control. The total/HDL cholesterol ratio was also found to be significantly different, decreasing the ratios in Go, Ex and GoEx. The hepatic TBARS increased significantly in gorup Ex (51.7+/-3.43 nM/g liver), while TBARS in Go and GoEx were low (35.68+/-3.61, 39.30+/-5.55 nM/g liver) and similar to control's one. The activity of hepatic SOD in Go and GoEx tended higher than control and Ex without garlic. The hepatic catalase showed significantly the highest activity in Go. Activity of GSH-px was significantly low in Ex with 0.14+/-0.03 unit/mg protein, and control, Go and GoEx had higher activities of 0.23+/-0.08, 0.20+/-0.07, 0.22+/-0.01 unit/mg protein, respectively. Lower activities of antioxidant enzymes in Ex are likely to associated with the highest level of TBARS. It seems that a decrease in TBARS in GoEx relative to Ex was related to the increase in GSH-px and SOD with garlic supplemented, which led to compensate the oxidative stress from exercise. The results sug-gests that exercise or garlic supplement exerts blood lipid attenuating effect. In adition, garlic supplementation could strengthen the antioxidant potential against exercise-induced oxidants, partly by modulating oxidant enzyme activity. These effects of garlic may make it a beneficial agent on CVD.
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Animals , Rats , Body Weight , Catalase , Cholesterol , Diet , Garlic , Oxidants , Oxidative Stress , Plasma , Rats, Sprague-Dawley , Swimming , Thiobarbituric Acid Reactive SubstancesABSTRACT
Vitamin E in the body system plays an important role in preventing chronic diseases by decreasing the oxidative stress by free-radicals. However, there are not enough researches on analyzing the primary factors affecting vitamin E levels in the blood in Korean adults. Therefore, the purpose of this research was to examine blood tocopherol levels and the primary factors affecting the status. A complete lifestyle survey was performed on 314 Korean adult men and surveyed their smoking, drinking and exercising habits. The average plasma level of alpha- and gamma-tocopherol showed similar mutual relations with plasma total cholesterol (TC), triglyceride (TG), or low density lipoprotein cholesterol (LDL-C) levels (p < 0.001). Plasma alpha-tocopherol level of the subjects did not show any difference as smoking, drinking and exercising habits changed. However, gamma-tocopherol per TG showed much lower figure in smokers than non smokers (p < 0.05). Amongst diet factors, plasma alpha-tocopherol level showed negative correlations with Vitamin E intake, while gamma-tocopherol level showed positive correlations with Vitamin E intake. Erythrocyte superoxide dismutase (SOD) activity and plasma tocopherol showed negative correlations, and catalase activity and plasma alpha-tocopherol showed positive correlationship. The level of cell DNA damage of lymphocyte and plasma alpha- or gamma-tocopherol showed negative correlations. As a result of this research, the factors that affect Korean adult men's plasma alpha-tocopherol level are plasma TG, LDL-C and cell DNA damage in lymphocyte, while the factors that affect gamma-tocopherol level are plasma TG, LDL-C and vitamin E intake based on multiple regression analysis. These findings implies that the level of different types of tocopherol depends on slightly different factors. A further research is needed on the factors involved in the differentiation of the types of tocopherol.
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Adult , Humans , Male , alpha-Tocopherol , Catalase , Cholesterol , Cholesterol, LDL , Chronic Disease , Diet , DNA Damage , Drinking , Erythrocytes , gamma-Tocopherol , Life Style , Lymphocytes , Oxidative Stress , Plasma , Smoke , Smoking , Superoxide Dismutase , Tocopherols , Triglycerides , Vitamin E , VitaminsABSTRACT
20 was lower than those whose BMI≤20(P
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Objective To investigate the effect of a novel isoflavone compound(F11) on the plasma lipid and cholesterol of the ovarectomied rat.Methods Female SD rats at age of 3 months old were randomly divided into 6 groups,that is,sham operation group(Sham),normal saline group(2 ml/d),estradiol group(E2,50 ?g?kg-1?d-1),and 3 F11 groups(15,50,150 mg?kg-1?d-1).Besides the Sham group,the ovary of the rats from other groups were resected,and received the injection as above mentioned.All rats were killed 10 weeks later,and their plasma lipid,total cholesterol,LDL,HDL,and body weight and uterine weight were measured.Results The plasma lipid,total cholesterol,LDL,HDL were significantly different in normal saline group and 4 treatment group(P
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Objective To study the distribution of IL-6 gene promoter 572C/G, 634C/G polymorphisms in Han population of Guangxi province, and analyze the relation between IL-6 gene polymorphisms and plasma lipid, platelet count. Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to detect IL-6 genotype in 198 healthy Han adults. At the same time the plasma lipid level and platelet count were determined by routine methods. Results Serum lipid levels had not significant difference among the different genotypes of IL-6 (P
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Statins is first choice of hypercholesteroiemia. Recently, clinical investigations have shown that statins can correct blood pressure in hypertension patients accompanied with lipid abnormalities. This result can be observed not only in untreated patients but also in patients treated with antihypertensive drugs. But some researches did not support this result completely. To some extend, the mechanisms responsible for the hypotensive effect seem to be largely independent on the effect of statins on lipid profile, and may be related to the improvement of endothelial function and the regulation of angiotensin II receptors.
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Objective To explore the curative effect of extracorporeal lipoprotein filter from plasma Delipid Plus(DELP) system therapy on acute cerebral infarction(ACI). Methods 36 patients with ACI were divided into DELP group (12 cases) and control group (24 cases). All of them received basic treatment including Aspirin and Pravastatin sodium etc. The DELP group also treated by DELP therapy twice. The scores of National Institute of Health Stroke Scale (NIHSS),Barthel Index (BI) and Modified Rankin Scale (mRS),and the levels of plasma fibrinogen (Fib),blood lipid and the indexes of hemorheology were compared pre and post treatment in the two groups.Results The reduction value of NIHSS between pre and post treatment in DELP group was bigger than this in control group(P
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Objective To investigate the relationship between carotid atherosclerosis(CAS)plaque and its related factors and cerebral infarction(CI).Methods The 91 patients with CI were detected by Color Doppler and the levels of plasma lipid,blood glucose(BG)and fibrinogen(Fbg)were evaluated,and compared with normal control(NC)group.Results(1)Compare with NC group,the IMT,prevalence of CAS plaque,the rates of soft plaque in CI group were significantly higher(all P
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AIM: To observe the effect of Tangmaiantai on the plasma lipid and renal function of rat with diabetic nephropathy. METHODS: The rat model of diabetic nephropathy was established by using STZ. The effect of Tangmaiantai on rat blood glucose before meal, serum TC, TG, Ccr, BUN and urinary protein were observed. RESULTS: Tangmainantai could decrease the level of serum TC, TG, Ccr, BUN and urinary protein, comparing to that of the model group (P
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The study was undertaken to ascertain the effects of C2 on he- patic lipase(HL), lipoprotein lipase ( LPL ) and plasma lipids. It was found that HDL-c and HDL-c /TC were increased, but(VLDL + LDL)-c and TC were decreased. LPL activety was positively correlated with the increase of HDL-c and the decrease of ( VLDL+LDL )-c.