Specific anti-toxocara IgG and IgE responses in the patients with ocular toxocariasis / 中国人兽共患病学报

To investigate the prevalence of specific anti-Toxocara IgG and IgE antibodies in ocular toxocariasis by means of ELISA and Western blotting, serum samples of 105 cases with uveitis of unidentified etiology were randomly selected from our stocked sera, which were referred to the Section of of Environmental Parasitology of Tokyo Medical and Dental University for detection of the anti-Toxocara antibody. By using ELISA, 82 of them (78.1%) were negative for both IgG and IgE antibodies, 12 (11.4%) were positive only for IgG, three (2.9%) were positive only for IgE, and eight (7.6%) were positive for IgG and IgE. Among the positive samples, as demonstrated by western bloting the IgG reacting bands were found to distribute in the whole range of molecular weights (97.2-14.3kDa)of excretory-secretory products of T. canis larvae. On the other hand,IgE antigenic molecules were concentrated on a relatively narrow range from 45kDa to 29kDa. In this study, we clearly demonstrated that some of the patients with uveitis showed specific anti-Toxocara IgE antibody but not IgG antibody in serum sample, suggesting that the demonstration of specific IgE antibody should be considered for the precise diagnosis of ocular toxocariasis. Further studies are needed to clarify the diagnosis significance of specific IgE antibody in ocular toxocariasis.

Testing the Human Serum for HBsAg by Ultrasensitive Chemiluminescent Photographic Detection-Dot-ELISA Technique / 中国输血杂志

The most new ultrasensitive chemiluminescent photographic detectiondot-ELISA (CPD-Dot-ELISA) technique was developed by combining the chemiluminescence technique with Dot-ELISA. The sensitivity of testing for pure HBsAg by CPD-Dot-ELISA was 30 and 60 times higher than by general Dot-ELISA and plate ELISA, respectively. 243 clinical serum specimens had been tested for HBsAg by both plate ELISA and CPD-Dot-ELISA, indicating that of 243 specimens tested for HBsAg by the former, 149 were positive, while of 243 specimens tested for HBsAg by the latter, 194 were positive. Of 149 positive specimmens tested by the former, only Ⅰ wasn't detected by the latter. 14 specimens randomly sampled from the additional 45 positive serum specimens detected by the latter, and 2 serum specimens which proved to be positive by both methods, had then been subject to neutralized test for HBIG, indicating that all 16 mentioned above specimens were positive. The results showed that the CPD-Dot-ELISA technique not only had its high sensitivity, good specificity and repro-ducibility, but also it was simple in manipulation, economically practical and worthy to be widely spread.