ABSTRACT
Objective To investigate whether PCF could inhibit apoptosis of HaCat cells induced by ultraviolet B(UVB) in vitro and explore its related mechanism.Methods HaCat cells were cultured and divided into groups(control,model,and test groups).The DNA ladder of each group was observed by gel electrophoresis;The expression of caspase-3 in HaCat cells was detected by flow cytometry and the activities of ERKs,JNKs,P38 were determined by western-blot method.Results PCF decresded UVB-induced DNA ladder in HaCat cells;PCF enhanced the level of ERKs,but reduced the level of JNKs and p38,and reduced the expression of Caspase-3 in HaCat cells.Conclusion PCF had protective effects against UVB-induced apoptotic cell death in HaCat cells and it is likely that its cytoprotective effect on HaCat cells was mediated by MAPKs pathway.
ABSTRACT
Objective To investigate the immunomodulation of PCF on the immunosuppressed mice. Methods The immunosuppressed model was established by injection of dexamethasone (DEX) intraperitoneally. Effects of PCF on the immunity were studied by the observation of morphology of immune organ, the analysis of T lymphocytes subsets and the measurement of lymphoproliferative ability. Results PCF could markedly improve the low immunoreaction of immunosuppressed mice caused by DEX. It was showed that the white pulp of spleen was enlarged, the quantity of T lymphocytes of the peripheral blood was increased and the response of lymphoproliferation to ConA was enhanced in the test group compared to those in the model group. Conclusion PCF had the abilities to up-regulate the immunity in vivo,which may be due to its influences on the peripheral mature immunocytes.
ABSTRACT
The polypeptides (PCF) were isolated from Chlamys farreri (Mr 800 -1000). The antioxidative mechanisms of PCF were studied in this paper. It has been shown that superoxide anion free radical and hydroxyl free radical were scavenged by PCF (3%, 15%). The oils and fats peroxide values (POV) and the contents of MDA were also determined. The results showed that the percentage scavenging superoxide anion free radical were 11- 7% and 41. 8% ,scavenging hydroxyl free radical were 8-6% and 38. 4% respectively. It could decrease the oils and fats POV and the contents of MDA in 5d and 10d(P