ABSTRACT
Colorants are mainly used to impart a distinctive appearance to the pharmaceutical dosage forms. The present study was carried out to develop aluminium lake color of a dye obtained from red cabbageusing different adsorbent (s) (Aluminium oxide or aluminium hydroxide or combination of both) in different ratio by simply mixing them to get dye adsorbed onto the surface of adsorbent.Batch Mode studies were carried out for 60 minutes. Aluminiumhydroxide at a concentration level of 30% w/v found to be the choice of adsorbent after optimization because maximum adsorption of dye from reaction mixture after 60 minutes and maximum % yield.After optimization of adsorbent the effect of pH and temperature also studied by formulating lake by varying these two parameters. It was observed that a higher temperature (50oC) and a slight acidic pH (6) favor the adsorption. The lake then characterized for various physicochemical properties like angle of repose, Carr’s index, hausner’s ratio, loss on drying, particle size and limit test for heavy metals. The lake was found to follow pseudo second order kinetics.
ABSTRACT
The adsorption kinetics and activities of endoglucanase enzyme from Rhizopus oryzae were evaluated using activated commercial charcoal as adsorbent. The effect of various experimental parameters on adsorption of endoglucanase such as initial enzyme concentration, amount of adsorbent, contact time and temperature were investigated. The pseudo-first-order and pseudo second-order kinetic models were used to describe the kinetic data which showed that the adsorption of the enzyme followed the pseudo-first order rate expression. The adsorbed enzyme was subjected to saccharification in presence of commercially available carboxy methyl cellulose and the reusability of the adsorbed enzyme was also tested.