ABSTRACT
Objective To investigate the changes of cytokines of splenocytes in mice immunized with recombinant BCG-Em Ⅱ/3 vaccine of Echinococcus multilocularis(Em)and consequently challenged with Em protoscoleces.Methods Balb/c mice were subcutaneously or intranasally vaccinated and challenged with Em were separated and cultured with EmAg,ConA or PHA,respectively.The supematants were gathered to measure the levels of IL-2,IFN-γ,TNF-α.and IL-4 by ELISA Kits.Results The levels of IL-2,IFN-γ,TNF-α and IL-4 in the subcutaneous group were(34.6±2.7),(34.5±2.8),(265.0 ±0.0)and(9.8±2.6)ng/L respeetively:those in the intranasal group were(32.5±2.2),(33.6±2.7),(130.0±0.0)and(10.4±27)ng/L respectively;those in the control were(25.0±1.9),(30.0±0.0),(10.0±0.0)and(12.5±2.7)ng/L,respectively:there were statistical differences between the immunized groups and control group(P＜0.01 or＜0.05);The level of TNF-α in the subcutaneous group was higher than that in the intranasal group.Conclusion Th1 response has been induced in mice vaccinated with rBCG-Em Ⅱ/3 vaccine and challenged with Em protoscoleces.
ABSTRACT
Objective:To investigate protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis(Em) and against challenge with Em protoscoleces. Methods:BALB/c mice were vaccinated by mix recombinant BCG vaccines subcutaneously and intranasally respectively,challenged with Em protoscoleces 8w after vaccination and killed 18w after infection to count rate of reduced alveolar echinococcus weight and to examine the level of IgE,IgG and its subclasses. Blank vector,BCG and PBS served as control. Results:In the groups of immunization.The rate of reduced alveolar echinococcus weight was 45.29%～76.47%,the level of IgG,IgG2a,IgG2b and IgE increased apparently,that of IgG1 and IgG3 decreased remarkably. Conclusion:Intranasal vaccination with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine may be a good route,IgG,IgG2a,IgG2b and IgE may play an important role in protection induced by this vaccine.
ABSTRACT
Objective:To dynamically observe changes of subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em).Methods:BALB/C mice were intranasally vacci- nated with the vaccine and killed to get spleen at 0,2,4,6,8,10,12,14,16 and 18w of immunization respectively.Splenocytes were.separated to measure subsets of CD_4~+ and CD_8~+T cells by FACsort,PBS served as control.Results:In the groups of im- munization,CD_4~+ and CD_8~+subsets increased obviously at 2～12w and 2-18w respectively,which reached the highest level at 6 and 10w respectively.Conclusion:The number of CD_4~+ subsets of splenocytes increases remarkably in the early stage (2～6week)by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine.
ABSTRACT
Objective To investigate the protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em) and against challenge of Em protoscolexes.Methods BALB/C mice were subcutaneously or intranasally vaccinated with mixed recombinant BCG vaccines respectively.Eight weeks later,the immunized mice were challenged with Em protoscolexes.On the 18th week after vaccination,the mice were killed to count the rate of reduced alveolar echinococcus weight and to examine the levels of IgG and its subclasses,IgE;the spleen cells were separated to measure subsets of CD4+ and CD8+ T cells by FACsort.Blank vector,BCG and PBS served as control.Results In the immunized groups,the rate of reduced alveolar echinococcus weight was 45.29%-76.47%;the levels of IgG,IgG2a,IgG2b and IgE increased apparently while IgG1 and IgG3 decreased remarkably;CD4+ subset and the ratio of CD4+/CD8+ increased obviously,but CD8+ subset had no change.Conclusion Intranasal vaccination with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine is a good way for immunization.IgG,IgG2a,IgG2b,IgE and CD4+ T cell subset may play an important role in protecting against challenge of Em protoscolexes.
ABSTRACT
Objective:To investigate changes in subsets of T cells in the spleen of mice by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em)and against challenge with Em protoscoleces.Methods:BALB/C mice were subcutaneously and intranasally vaccinated with mix recombinant BCG vaccines respectively,then challenged with Em protoscolexes on 8w of vaccination and killed on 18w of infection to get spleen.Spleen cells were separated to measure subsets of CD4+ and CD8+T cells by FACsort,Blank vector,BCG and PBS served as control.Results:In the groups of immunization,CD4+subset increased significantly,as well as the ratio of CD4+/CD8+subset,but CD8+ subset had no change,CD4+ subset and ratio of CD4+/CD8+subset in the subcutaneous group were higher than these in the intranasal group.Conclusion:CD4+T cells subset may bear a part in protection induced in mice by mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine against challenge with Em protoscoleces.