ABSTRACT
The present paper aims to develop a simple direct colorimetric method for the determination of cerberin in rat plasma without any previous chemical separation of the cerberin from Cerbera odollam. The method is based on reaction of the cardenolide group of cerberin with 3,5-dinitro salicylic acid [DNS] in alkaline medium which yields a bright orange-yellow complex that exhibits absorption maxima at 370nm.Beer’s law obeyed in the concentration range of 50-250μg/mL. The result of the method was validated statistically and by recovery studies.
ABSTRACT
Two simple and sensitive visible spectrophotometric methods (A and B) have been developed for the determination of sumatriptan succinate in bulk and tablet dosage forms. Methods (A and B) are based on the reaction of drug with aromatic aldehydes such as Vanillin or Para dimethyl amino cinnamaldehyde(PDAC) in the presence of sulphuric acid in non aqueous medium and formed purple red colored condensation products with an absorption maximum of 565nm for method A and 560nm for method B. The Beer’s law obeyed in the concentration range of 10-50μg/ml for method A and 20-60μg/ml for method B. The proposed methods are applied to commercial available tablets and the results are statistically compared with those obtained by the reference method and validated by recovery studies.
ABSTRACT
Este trabalho apresenta uma modificação dos procedimentos descritos nas Farmacopéias Francesa e Européia para a análise de flavonoides de Passiflora incarnata L., Passifloraceae, por espectrometria UV-Visível e propõe a sua aplicação na determinação dos flavonoides totais das folhas da espinheira-santa (Maytenus aquifolium Mart. e Maytenus ilicifolia (Schrad.) Planch., Celastraceae) e do maracujá (Passiflora edulis Sims. e Passiflora alata Curtis, Passifloraceae). Os resultados obtidos por espectrometria no UV-Visível foram comparados aos obtidos por cromatografia líquida de alta eficiência (CLAE-UV), encontrando-se resultados estatisticamente similares entre os métodos espectrométrico modificado da Farmacopéia Francesa e CLAE-UV.
This paper reports on a modification of the spectrometric procedures originally described in the French and European Pharmacopoeia for the analysis of Passiflora incarnata L. (Passifloraceae) flavonoids, proposing its application in the determination of total flavonoids from "espinheira-santa" (Maytenus aquifolium Mart. and Maytenus ilicifolia (Schrad.) Planch., Celastraceae) and "maracujá" leaves (Passiflora edulis Sims and Passiflora alata Curtis, Passifloraceae). A comparison was made of the results obtained by the spectrometric procedure with those obtained by high performance liquid chromatography (HPLC-UV), which demonstrated complete compatibility between the modified French Pharmacopoeia (spectrometric) and HPLC-UV methods.