ABSTRACT
Objective:To investigate the expression of zinc finger protein 22 (ZNF22) gene in hepatocellular carcinoma (HCC) and its effect on tumor proliferation, apoptosis, invasion and metastasis of HCC.Methods:The expression of ZNF22 in 32 HCC specimens, and 371 HCC samples from the cancer genome atlas database were analyzed. ZNF22 knockdown and negative control SNU-449 and JHH-7 HCC cell lines were constructed. The effects of ZNF22 on HCC cells were observed by cell proliferation assay, plate clone formation assay, apoptosis assay, scratch healing assay, Transwell invasion assay, subcutaneous tumor formation, tail vein injection transfer, and small animal live imaging assay in nude mice.Results:The expression of ZNF22 gene is higher in HCC tissues than in paracellular carcinoma tissues, and the difference was statistically significant ( P<0.001). The growth rate of SNU-449 and JHH-7 cells in ZNF22 knockdown group was lower than that in control group, and the difference was statistically significant ( P<0.001). Compared with negative control group, the clone number formed by SNU-449 cells in ZNF22 knockdown group decreased (26±8 vs. 59±5, P<0.01), the level of apoptosis increased (6.60%±0.22% vs. 2.38%±0.30%, P<0.001), the migration rate decreased (14.47%±6.42% vs. 68.84%±8.01%, P<0.001), and the number of invasive cells decreased (48.00±2.23 vs. 179.00±4.81, P<0.001). There was no obvious tumor growth after subcutaneous injection of JHH-7 cells into nude mice in ZNF22 knockdown group, and the systemic fluorescence expression was lower than that of the negative control group, and the difference was statistically significant ( P<0.05). No metastases were observed on autopsy in knockdown group nude mice. Conclusion:ZNF22 is highly expressed in HCC while knockdowing ZNF22 gene inhibited the growth, proliferation, invasion, metastasis of HCC cells, and induced apoptosis of HCC cells.
ABSTRACT
Partial hydatidiform mole can evolve into a metastatic trophoblastic tumor. A 36-year-old, multiparous woman, pregnant with a 22-week embryonic hydatidiform mole, having spontaneously expelled. Histopathological examination showed a non-invasive partial mole. During biological monitoring, a trophoblastic tumor was diagnosed with pulmonary metastasis on CT-scan and myometrial invasion by MRI. Authors opted for a monochemotherapy with a good evolution. The potential risk of malignant transformation of the partial hydatidiform mole requires an adequate therapeutic strategy with strict monitoring.
ABSTRACT
Epithelial membrane protein 3 (EMP3) is a trans-membrane signaling molecule with important roles in the regulation of apoptosis, differentiation and invasion of cancer cells, but the detailed is largely still unknown. We analyzed the mRNA levels and methylation statuses of EMP3 in 63 primary breast carcinomas and assessed their correlations with clinicopathologic variables. The expression of EMP3 mRNA in primary breast carcinomas was significantly higher than the expression of 20 normal breast tissues (p<10(-7)). EMP3 overexpression in breast carcinomas was significantly related to histological grade III (p=3.9X10(-7)), lymph node metastasis (p= 0.003), and strong Her-2 expression (p=3.3X10(-6)). Hypermethylation frequencies of EMP3 were detected in 36.5% of breast carcinomas by methylation-specific polymerase chain reaction. However, no significant correlations were found between methylation status of EMP3 and mRNA expression levels as well as other clinical parameters. In conclusion, EMP3 may be a novel marker of tumor aggressiveness. Overexpression of EMP3 in primary breast carcinoma is not associated with DNA methylation.
Subject(s)
Adult , Female , Humans , Middle Aged , Breast Neoplasms/genetics , Carcinoma/genetics , DNA Methylation , Epigenesis, Genetic , Gene Expression Regulation, Neoplastic , Lymphatic Metastasis , Membrane Glycoproteins/genetics , Neoplasm Staging , RNA, Messenger/metabolism , Receptor, ErbB-2/genetics , Severity of Illness IndexABSTRACT
Objective To investigate the effect of Chinese herbal medicine of astringents on H22 ascitic type hepatoma haematogenous and lymphatic metastasis in mice.Methods In hematogenous metastasis experiment,the growth of lung metas- tasis and hemorheological indexes were observed in Kunming mice after intravenous injection of H22 ascitic type hepatoma into the caudal veins.In lymphatic metastasis experiment,hepatocarcinoma metastasis mice models were established by subcuta- neous injection of H22 hepatocareinoma cells into the right lower limb,and the inhibitory- rate of tumor weight,incidence of metastasis carcinoma in lung,metastasis of lymph nodes were observed.Results In hematogenous metastasis experiment, compared with the model group,the number of metastatic nodules in lung was decreased in Cortex Ailanthi group and Fructus Corni group,and Cortex Ailanthi improved the hemorheological indexes.In lymphatic metastasis experiment,compared with the control group,the number of metastatic nodules in lung was decreased and the inhibitory rate of tumor weight was increased in Fructus Mume group and Fructus Corni group.The metastases of lymph node were decreased in the three astringents groups. Conclusion Chinese herbal medicine of astringents has an inhibitory effect on the metastasis of hepatoma haematogenous and lymphatic metastasis.