Study of fingerprints pattern among some saudis of different ABO blood groups

Fingerprint evidence is undoubtedly the most reliable and acceptable evidence till date in the court of law. Due to the immense potential of fingerprints as an effective method of identification an attempt has been made in the present work to: 1- Study the distribution of fingerprint pattern among the participants having different ABO and Rh blood groups. 2- Study any relationship between their characters and blood groups. A cross-sectional study was conducted on 310 male students aged from 22-30 years in the first grade of the King-Fahad Security College Riyadh, Saudi Arabia. This study was carried out over a period of 3 months from March to May 2011. The plain fingerprints of all the ten digits were taken separately on the respective blocks on the sheet of paper. The details of their blood group were registered from their college identity cards. Statistical analysis of the obtained data showed that the majority of the subjects [54.5%] in the study were of blood group O followed by blood group A [28.8%], then blood group B [13.5%] and AB [3.2%]. Rh positive cases constituted about [91.9%] of all the studied cases. The general distribution pattern of fingerprints showed high frequency of loops[48.1%] followed by whorls [47.0%] and arches [4.9%]. There was a statistical significant difference among blood groups as regards pattern of fingerprints. Whorls were the commonest type of fingerprints in participants with blood groups A [48.3%], B[54.0%] and AB [59.0%] while, loops were the predominant pattern among participants with blood group O [50.2%]. Whorls are the predominant pattern of fingerprints in participants with Rh positive and Rh negative blood groups A [47.8% and 53.8%], B [54.9% and 48.0%], AB [56.3% and 70.0%] respectively and in Rh negative blood group O [53.0%]. Otherwise loops predominated in Rh positive blood group O [50.6%].Whorls were the most frequent fingerprint pattern in all types of ABO blood groups as regards ring, index and thumb fingers. The total fingers ridges count [TFRC] was significantly greater in blood group O. The study suggests relationship between fingerprints pattern and blood groups. We recommend further studies to be done on larger samples

Digital dermatoglyphis in ABO, Rh blood groups.

Dermatoglyphics, the study of fingerprints are constant and individualistic. The ridge pattern depends upon cornified layer of epidermis as well as dermal papillae. This study was conducted to correlate between digital dermatoglyphics patterns in ABO, Rh blood groups and evaluates their significance. A total of 200 first year MBBS students with known blood groups from 2004 and 2005 batch of IGGMC, Nagpur were included in the study. Fingerprints were obtained by printing method. Parameters studied were arches, whorls, loops. It was concluded that, whorls were highest in B blood group and the difference was significant with O blood group. Loops were highest in O blood group and were significant with A, B, AB blood groups. Arches were highest in AB blood group and were statistically significant with B and O blood groups. Arches were higher in Rh negative blood group differing statistically with Rh positive blood group.

ABO blood groups and Rhesus factor: An exploring link to periodontal diseases.

Background: The presence or absence of blood group antigens has been associated with various diseases, with antigens also acting as receptors for infectious agents. Scanty literature is available in assessing the relative liability of blood group phenotypes to periodontal diseases. This research was conducted to determine the association of the ABO blood group and Rhesus (Rh) factor to periodontal diseases to assess whether they could be the predictors of periodontal diseases. Materials and Methods: A total of 1,220 subjects aged between 20 and 55 years were selected on a random basis. The study populations were segregated into three groups according to Ramfjord's periodontal disease index: Healthy, Gingivitis and Periodontitis. Blood samples were collected to identify the ABO blood groups and the Rh factor by the slide method. Results: Blood group A showed a significantly higher percentage in the gingivitis group and blood group O showed a higher percentage in the periodontitis group. The blood group AB showed the least percentage of periodontal diseases. The distribution of Rh factor in all groups showed a significantly higher distribution of Rh-positive. Conclusion: The genetic factors may alter the oral ecology and the process of periodontal disease. These data are suggestive of a broad correlation between periodontal diseases and blood groups, which may act as risk predictors for periodontal diseases. This will make it possible to better-understand the risk factors of diseases of the periodontal tissues and to predict the effective methods of prevention and treatment of periodontal diseases.

ABO genotyping in leukemia patients reveals new ABO variant alleles

The ABO blood group is the most important blood group system in transfusion medicine and organ transplantation. To date, more than 160 ABO alleles have been identified by molecular investigation. Almost all ABO genotyping studies have been performed in blood donors and families and for investigation of ABO subgroups detected serologically. The aim of the present study was to perform ABO genotyping in patients with leukemia. Blood samples were collected from 108 Brazilian patients with chronic myeloid leukemia (N = 69), chronic lymphoid leukemia (N = 13), acute myeloid leukemia (N = 15), and acute lymphoid leukemia (N = 11). ABO genotyping was carried out using allele specific primer polymerase chain reaction followed by DNA sequencing. ABO*O01 was the most common allele found, followed by ABO*O22 and by ABO*A103. We identified 22 new ABO* variants in the coding region of the ABO gene in 25 individuals with leukemia (23.2%). The majority of ABO variants was detected in O alleles (15/60.0%). In 5 of 51 samples typed as blood group O (9.8%), we found non-deletional ABO*O alleles. Elucidation of the diversity of this gene in leukemia and in other diseases is important for the determination of the effect of changes in an amino acid residue on the specificity and activity of ABO glycosyltransferases and their function. In conclusion, this is the first report of a large number of patients with leukemia genotyped for ABO. The findings of this study indicate that there is a high level of recombinant activity in the ABO gene in leukemia patients, revealing new ABO variants.

A Case of ABO*Ael02/O04 Genotype with Typical Phenotype O / 대한진단검사의학회지

Ael is a rare blood type which has the least amount of A antigen among A subgroups. It can be detected by special tests performed to resolve the discrepancy between red cell and serum typing in routine serological typing. The presence of A antigen on Ael red cell is demonstrable only by adsorption and elution tests. An Ael individual does not secret A substance in the saliva and may have anti-A antibody in the serum which is usually less reactive with the reagent red cells than anti-B antibody. In Korea, Ael02 has been reported more frequently than other Ael alleles. We report a case of Ael02/O04 who presented as typical phenotype O with strong anti-A and anti-B antibodies and no A antigen detected even by adsorption and elution tests. The case has been proved to be Ael02/O04 by direct sequencing analysis. In individuals with history of discrepancies in the results of ABO phenotyping, ABO genotyping is needed for an accurate evaluation of their blood type.

Association of blood group A with increased risk of coronary heart disease in the Pakistani population

Many reports have appeared in recent years showing an association between blood groups and cardiovascular diseases. Clinical studies in developed countries have shown that individuals of the A blood group phenotype are more susceptible to Coronary Heart Disease [CHD]. The present study was designed to investigate the correlation of ABO blood groups and CHD in the South Asian population. 252 CHD patients and 75 healthy controls were recruited from Karachi and Nawabshah, Pakistan. The results obtained in this study show that the prevalence of CHD in blood group A is invariably higher than in all other ABO blood groups. It is striking that despite the fact that the most prevalent blood group among Pakistanis is phenotype B, the incidence of CHD is highest in individuals with blood group phenotype A. This suggests that a certain CHD risk is associated with phenotype A. Thus, we conclude that, in the Pakistani cohort investigated in the present study, blood group phenotype A is associated with a substantially increased risk for CHD, which seems independent of conventional cardiovascular risk factors

Reactivo hemoclasificador monoclonal cubano Hemo-Cim anti-A. Estudio de estabilidad / Cuba anti-A Hemo-Cim hemoclassifier monoclonal reagent. Stability study

Los estudios de estabilidad de los reactivos fabricados a partir de anticuerpos monoclonales (AcM) de origen murino, son esenciales para obtener resultados adecuados en su aplicación práctica y constituyen un requisito indispensable en las buenas prácticas de producción, lo que permite establecer adecuadamente la vida de estos productos. Se usaron los métodos de hemaglutinación recomendados para medir la actividad biológica del producto Hemo-CIM anti-A expresada en la potencia, la avidez y la intensidad frente a un panel de eritrocitos del grupo A1 y A2B, que se incluyó por su baja expresión del antígeno A para demostrar más efectivamente cualquier deterioro que sufriera el reactivo. Se estableció que la vida útil del reactivo hemoclasificador producido en el Centro de Inmunología Molecular es de 2 años y se determinó que la temperatura de almacenamiento del producto está entre 2 y 8 ºC. Además, los lotes que se colocaron diariamente en la meseta de trabajo durante 5 horas a 21 ºC a partir del mes 0 y a los 8, 14 y 22 meses después de su producción, simulando las condiciones a la que los usuarios someten a estos reactivos, mantuvieron las características de calidad que se requieren para su uso, lo que demostró que con este producto se puede trabajar en esas condiciones

Identificación molecular del Gen RhD mediante PCR / Molecular identification of the RhD gene by PCR

Introducción. Con objeto de identificar al gen Rh(D) en sujetos positivo, se elaboró una estrategia basada en la reacción en cadena de la polimerasa (PCR). Material y métodos. Se estudiaron a 8 sujetos adultos, identificando el fenotipo del sistema Rh (CcDEe) con técnicas inmunohematológicas. Se extrajo el DNA genómico a partir de leucocitos de sangre periférica y se utilizaron dos iniciadores (24C y R13N) para la amplificación. Este par de iniciadores delimitan las secuencias comunes entre los exones 4 y 5 de los genes RhD y RhCE. Resultados. Inmunológicamente se identificaron cinco sujetos Rh negativo y tres Rh positivo. En la imagen electroforética, se observaron dos patrones de amplificación: uno de dos bandas (de 600 pb y de 1200 pb) y un segundo patrón de una sola banda (de 1200 pb). La banda de 1200 pb se amplificó en los sujetos Rh positivo y negativo (y corresponde al gen RhCE). La banda de 600 pb, sólo se presentó en sujetos Rh positivo (correspondiente al gen RhD). No hubo diferencias entre los resultados inmunohematológicos y el análisis molecular en los diferentes fenotipos. Conclusiones. El empleo de esta estrategia basada en la técnica de PCR, permite la tipificación del gen RhD en los sujetos Rh positivo, con diferentes fenotipos del sistema Rh

A subgroups of the ABO blood system

The A and AB variants of the ABO blood group system were studied in a sample of 367 donors from Belém (Par State, Brazil), and in 5 families of donors in which variation was observed. Tests were based on commercial anti-A and anti-B sera, and anti-A1, anti-H lectins. Observed subgroups and their frequencies were: A1 (235); Aint (11); A2 (Hw (31); A2 (66); A1B (11); Aint B (1); A2(BHw (6); A2B (5); A3B (1). No statistically significant differences were observed in the distribution of variants according to racial group. Overall, the observed variation in the AH/ABH condition may reflect ethnic differences between individuals, given that the population of Belén is trihybrid.

Risk estimation of ABO and Rho(D) incompatibility in persons with mono- and polyphyletic surnames in Monterrey, Mexico: comparison with other Mexican population

ABO and Rho(D) blood groups were determined in 3813 males and females affiliated with the Instituto Mexicano del Seguro Social (IMSS) who are residents of the Monterrey Metropolitan Area (MMA) in northeastern Mexico. They were selected by their monophyletic or polyphyletic surnames. The ABO and Rho(D) blood group phenotypes and gene frequencies were determined and based upon these, the risk of incompatibilities was estimated for both marriages (MI) and maternal-fetal incompatibility (MFI). These were compared with those estimated for other populations of residents in the MMA, and in other locations in Mexico, as well as with the two most important ancestral populations, Spanish and Tlaxcaltecan Mexican Indians, with the hypothesis that the percent of risk ABO and Rho(D) MI and MFI are greater in the population with monophyletic surnames than those with polyphyletic surnames. It was found that for persons with nomophyletic and polyphyletic surnames, as well as for the other populations in the MMA and other places in Mexico, their ABO and Rho(D) MI and MFI percent of risk are intermediate to the ones estimated for their ancestry. The percentages of MI and MFI are higher for the persons with monophyletic than for the ones with polyphyletic surnames, other populations from the MMA and those from other locations in Mexico. The risks are higher when the similarity with Spanish increases and are lower when their similarities with the mexican Indians increase