ABSTRACT
Methyleugenol is naturally occurring substance in oils and fruits and in various foods as flavoring agent. Effect of this methyleugenol in inhibiting A. flavus colonization and aflatoxin production on peanut pods and kernels has been studied. Spray of methyleugenol (0.5%) on peanut pods and kernels checked the colonization of A. flavus and aflatoxin synthesis. This chemical can be used as both prophylactic or post infection spray on peanut pods before storage. It is the first report on the inhibition of A. flavus by methyleugenol on peanut.
Subject(s)
Aflatoxin B1/biosynthesis , Antifungal Agents/pharmacology , Arachis/drug effects , Arachis/microbiology , Aspergillus flavus/drug effects , Aspergillus flavus/growth & development , Biological Assay , Colony Count, Microbial , Eugenol/analogs & derivatives , Eugenol/pharmacology , Microbial Sensitivity TestsABSTRACT
Avaliou-se a produçäo de aflatoxina B1 pelo Aspergillus flavus IMI 190443 em sementes de amendoim, genótipos Tatu Vermelho e VRR-245, cultivados no Centro Experimental do Instituto Agronômico de Campinas em 1994/1995 e submetidos a adubaçäo com sulfato ferroso. Após a colheita, os gräos de amendoim foram previamente autoclavados a 121§C por minutos, e inoculados com uma suspensäo de esporos de Aspergillus flavus IMI 190443, em três concentraçöes: 10(3), 10(4), 10(5) esporos/mL. Observou-se uma menor produçäo de aflatoxina B1 no genótipo VRR-245, independente da concentraçäo de esporos de Aspergillus flavus IMI 190443 (10(3), 10(4), e 10(5) esporos/mL) e do tratamento com e sem sulfato ferroso (p<0,05), quando comparado ao Tatu Vermelho. Verificou-se uma menor contaminaçäo com aflatoxina B1 nos genótipos Tatu Vermelho e VRR-245, após adubaçäo e inoculaçäo com Aspergillus flavus IMI 190443, apenas na concentraçäo de 10(3) esporos/mL