ABSTRACT
OBJECTIVE@#To investigate the relationship between tryptase in serum and anaphylaxis.@*METHODS@#The concentrations of tryptase in the sera of heart blood in three persons died from anaphylaxis shock were detected by ELISA. The first sample was obtained from a man, aged 38, died of injecting Amikacin. The second sample was obtained from a man, aged 42, died of injecting Cephradine. The third sample was from a woman, aged 39, died of injecting Lincomycin. All samples were stored in -20 degrees C.@*RESULTS@#The concentrations of tryptase in sera were 52 ng/ml, 121 ng/ml and 0.73 ng/ml. It was unknown why the concentration of tryptase in the third sample was normal.@*CONCLUSION@#In fetal anaphylaxia reaction tryptase measurement is a useful indicator, but the diagnosis is not to be based on the test alone.
Subject(s)
Adult , Female , Humans , Male , Anaphylaxis/enzymology , Biomarkers/blood , Cause of Death , Enzyme-Linked Immunosorbent Assay , Forensic Medicine , Mast Cells/enzymology , Postmortem Changes , Retrospective Studies , Serine Endopeptidases/blood , TryptasesABSTRACT
OBJECTIVE@#To investigate the changes of the activity of tryptase of sera, lungs and bronchial tubes in the guinea-pigs which suffered from hetero-serum anaphylactic shock.@*METHODS@#Sera and tissues were collected from anaphylactic shock guinea-pigs, and the enzyme activity was tested colormetrically using special substrate, BAPNA.@*RESULTS@#The activity of tryptase of sera, lungs and bronchial tubes increased significantly in Anaphylactic guinea-pigs compared with control group.@*CONCLUSION@#The changes of tryptase activity are helpful to diagnose anaphylactic shock.
Subject(s)
Animals , Female , Male , Anaphylaxis/enzymology , Forensic Medicine , Guinea Pigs , Serine Endopeptidases/metabolism , TryptasesABSTRACT
Injection of heparin as well as antigenic challenge in sensitized guinea pigs are known to produce a release of histaminase into the plasma. In the present study, tissue histaminase estimation was done by Spencer's method (17) and plasma estimation by Kapellar Adler's (7) method. Mepyramine pretreatment considerably decreased the histaminase release by both heparin and anaphylaxis. Mepyramine did not antagonise the anticoagulant action of heparin in vitro.