ABSTRACT
The immune response elicited by the oral inoculation of an intermediate strain of infectious bursal disease virus was studied in chickens. A strong over expression of IL-6, IL-8, IFNα and IFNγ was observed in bursa at 3 days post inoculation together with an increase in splenic NO2 release. An influx of T-lymphocytes was also detected.
Subject(s)
Animals , Birnaviridae Infections/veterinary , Chickens , Infectious bursal disease virus/immunology , Poultry Diseases/immunology , Administration, Oral , Birnaviridae Infections/immunology , Bursa of Fabricius/pathology , Cytokines/analysis , Cytokines/genetics , Gene Expression Profiling , Nitric Oxide/analysis , Spleen/pathology , T-Lymphocytes/immunologyABSTRACT
The effects of Dangguibuxue Tang (DBT) on growth performance and immunity response in immunosuppressed broiler chicks were investigated in this study. 240 one-d-old broiler chicks (DaHeng S01) were randomly divided into 4 groups, 2.0% DBT-treatment (A), 0.5% DBT-treatment (B), cyclophosphamide-control (C), and control group (D). From 4 d to 7 d of age, chicks in group A, B and C were given cyclophosphamide (CY) at a dosage of 100mg/kg body weight (BW) daily by intraperitoneal injection to induce immunosuppression. Chicks in group D were given an equal volume of physiological saline daily by intraperitoneal injection and considered normal chicks. Groups A and B were supplemented with 2.0% or 0.5% of DBT in the drinking water from 8 d to 42 d of age. Groups C and D did not receive any additional medication. The results revealed that chicks from group B had lower feed:gain rate (FGR), lower total mortality, higher immunity organ indexes, higher levels of Newcastle disease (ND) antibody and infectious bursal disease (IBD) antibody, higher interleukin-2 and interleukin-6 levels, and greater lymphocyte proliferative responses to concanavalin A (ConA) during the experiment than those from group C. However, no significant difference in the immunity status in the two levels of DBT-treatment was observed. These results indicate that supplementation of 0.5% of DBT can improve both cellular immunity and humoral immunity in immunosuppressed broiler chicks.
Subject(s)
Animals , Female , Birnaviridae Infections/veterinary , Chickens , Drugs, Chinese Herbal/pharmacology , Infectious bursal disease virus/immunology , Newcastle Disease/immunology , Angelica sinensis , Astragalus Plant , Birnaviridae Infections/immunology , Chickens/growth & development , Chickens/immunology , Cyclophosphamide/pharmacology , Immunosuppression Therapy/methods , Immunosuppression Therapy/veterinary , Immunosuppressive Agents/pharmacology , /blood , /blood , Random AllocationABSTRACT
A trial for emergency vaccination against lBDV 48-hour post experimental infection of 29-day old immunostimualnts, namely; Pind-Avi [Fowl pox virus inactivated by gamma rays], Ultracorn[R] [Corynebacterium cutis lysate], Levamisole hydrochloride [R] and transferrin. The results revealed that emergency vaccination of infected chicks was useless in controlling mortality or bursal damage [which was evidenced by bursal lesion score and bursal lymphoid tissue lesions]. The use of these immunostmulants alone improved the protection% but had not restored the bursal damage with varying degree of stimulation of non-specific immune response in the following order: Transferrin, ultracorn [R], Pind-Avi finally levamisole. The combination of emergency vaccine with immunostimulant didn't show a significant control point