Helicobacter Pylory infection in patients with esophageal squamous cell carcinoma

OBJECTIVE: Esophageal squamous cell carcinoma is one of the most common esophageal diseases in the developing world, but the relationship between esophageal squamous cell carcinoma and Helicobacter pylori infection remains a neglected topic. The primary objective of this study was to determine the association between Helicobacter pylori infection and esophageal squamous cell carcinoma. A second purpose was to determine the incidence and factors associated with Helicobacter pylori infection following esophagectomy. METHOD: The microorganism was identified by testing the gastric biopsy materials from 95 esophageal squamous cell carcinoma patients (66 females; 39 were esophagectomized) for urease activity in a medium containing urea and a power of hydrogen detection reagent and comparing the results with those from a healthy population. Differences in patient characteristics were assessed with chi-square tests and t-tests for categorical and continuous factors, respectively. RESULTS: The patients with esophageal squamous cell carcinoma had a significantly lower prevalence of Helicobacter pylori compared with the healthy population (p<0.001). The naive and esophagectomized patients, in contrast, showed no significant differences in Helicobacter pylori infection (p>0.005). Patients with esophageal squamous cell carcinoma showed a significant association between leukocytosis and hypoglobulinemia and the presence of Helicobacter pylori infection (p=0.023 and p=0.045, respectively). CONCLUSION: These results suggest that Helicobacter pylori is not an etiological factor in patients with esophageal squamous cell carcinoma. We found a statistically significant negative correlation between esophageal squamous cell cancer and Helicobacter pylori infection. These findings may guide new strategies for esophageal squamous cell carcinoma therapy.

Molecular detection of human papillomavirus type-16 DNA in cervical cancer tissue biopsies

The aim of the study is to detect the frequency of human papillomavirus type-16 among patients with cervical carcinoma. Khartoum Hospital, Army Medical Hospital and Soba University Hospital, Sudan. Descriptive-cross sectional study. Fifty specimens of treated cervical biopsy sections [Paraffin embedded] were included in the study from April to October 2012. DNA was extracted followed by the detection of E6 gene of human papillomavirus type-16 using non-probed SYBER green real-time PCR. Thirty [60%] showed positive results as compared with the sigmoid curve of the positive control for HPV type-16; while 20 [40%] were negative. Most of the positive results were among the age group 31-50 years. Human papillomavirus type-16 was detected in 60% of women with cervical cancer, which seems to have a strong association with cancer development.

Lack of association between Helicobacter pylori and laryngeal carcinoma.

OBJECTIVE: To survey the role of Helicobacter pylori at the tissue level as a cause of squamous cell carcinoma of the larynx. DESIGN: A case-control study. SETTING: In an Otolaryngology Ward at an academic university. SUBJECTS: Patients with laryngeal cancer as cases and patients with benign laryngeal lesion as controls. MAIN OUTCOME MEASURE: In all subjects, specimens of laryngeal tissue were examined by rapid urease test while histopathologic examination was achieved to detect H. Pylori. RESULTS: Totally, 44 patients (42 men and 2 women) with squamous cell carcinoma of larynx and 30 patients (24 men and 6 women) with benign laryngeal lesions (polyp, nodule, granuloma) were studied, none of which were infected with the bacterium. CONCLUSION: Our results did not show H. Pylori infection among patients with laryngeal cancer (SCC) or benign laryngeal lesions. .

[ correlation between helicobacter pylori infection and squamous cell carcinoma of larynx and hypopharynx]

From the most important laryngeal and hypopharyngeal cancer risk factors are smoking, alcohol and anemia. But reflux is one of the suggested etiologies. Pregnancy, Nonstroidal anti inflammation drugs and drugs decreasing the lower esophageal sphincter pressure will increase the reflux. Helicobacter pylories role in reflux formation is different due to the involved region and can also have a decreasing role and decline the rate of a series of esophageal and upper aero digestive system disease. This cross - sectional and case - control study was done in Qaem medical hospital, Mashhad during the years 1384 and 1385, for serum level detection of anti -H.pylori IgG and IgA antibodies in blood samples of both control and case groups. The variables were age, sex and serum test results. 45 patients [39 male and 6 female] and 42 controls [33 male and 9 female] were evaluated. 2 groups were matched for age and sex. Positive IgG and IgA results in patients in order was 3 person [6.6 percent] and 3 person [6.6 person] and in control group 26 person [61.9%] and 12 person [28.5%]. Result [P>0.001] was for healthy group showing the protective effect of H. Pylori against laryngeal cancer. In our study, control group with a meaningful difference was affected by H.Pylori infection. This can be due to a protective effect for H. Pylori against the laryngeal and hypopharyngeal malignancies. Prospective studies and simultaneously evaluation of reflux, H. Pylori infection and the regain of gastric location are suggested

Squamous cell carcinoma arising in chronic ulcers in leprosy.

Squamous cell carcinoma (SCC) arising in chronic ulcers of leprosy patients is uncommon. A retrospective analytical study of 416 biopsy specimens of chronic ulcers during 1976 - 2000 occuring on the extremity of leprosy patients was performed. Pathologic examination showed that 217 specimens (52.2%) were reported as pseudoepitheliomatous hyperplasia (PH). A total of 102 (24.5%) specimens reported as SCC, involving 100 individual patients (69 males, 31 females). SCC was localized on the lower extremity in 87 specimens (85.3%). No significant difference was observed between left and right foot using Chi-square test. The sole was the commonest site of involvement, and then the heel. SCC on the upper extremity which was much less common, presented in 15 specimens (14.7%). The tumor was localized on the palm in 10 specimens (right: 9, left: 1). SCC in chronic ulcers of leprosy patients were much more common on the lower extremity than on the upper extremity. Most of the tumors on the upper extremity were localized on the right palm. This emphasizes the need for an active policy of prevention of disability in leprosy control programs.

Absence of Helicobacter pylori in oral mucosal lesions.

A prospective study was conducted in 26 patients with benign oral ulcers and oral carcinoma and 26 age and sex matched controls to determine the prevalence of Helicobacter pylori in oral mucosal biopsies. Oral mucosal biopsies were subjected to rapid urease test, campylobacter like organism (CLO) test, histopathological examination and bacteriological culture for demonstration of H pylori. Urease test was positive for H pylori in 3 (11.4%) out of 26 cases and CLO test in 2 (14%) out of 14 cases. On histology, H pylori was identified in 4 (15.38%) out of 26 cases. The spiral organism was universally absent in culture of both patients and controls. These pilot data negate the casual association of H pylori in oral mucosal lesions.

Detection of oncogenic HPV DNA by a consensus polymerase chain reaction method in genital carcinomas in twenty women in Barbados

Paraffinized tissue from Barbadian women with histologically proven gential carcinoma was subjected to a censensus polymerase chain reaction method. Nineteen patients had cervical and one, vaginal carcinoma. The histological types were 17 squamous cell carcinoma, 2 adenocarcinoma and 1 adenosquamous carcinoma. HPVDNA was detected in 18/20 (90 per cent ). HPVDNA type 16 in 13 (65 per cent ), type 33 and type 45 in 1 (5 per cent ) each and 3 (15 per cent ) could not be typed. HPVDNA, type 16, was detected in one (50 per cent ) of the two cases of adenocarcinoma and 12/17 (71 per cent ) cases of squamous cell carcinoma. DNAHPV, type 33, and type 45 were each detected in 1/17 (6 per cent ) cases of squamous cell carcinoma. No HPVDNA, type 18, was detected.

Aspectos dos quadros clínico, citológico e da microbiota fúngica da mucosa bucal de pacientes com carcinoma da boca, antes e durante a radioterapia / Aspects of the clinical diagnosis, cytological and of oral mucosa of patients with oral cancer, before and during the radiotherapy

Os autores estudaram clinicamente as lesöes e alteraçöes observadas na mucosa bucal de pacientes com carcinoma da boca, independente da neoplasia presente, antes e durante o tratamento radioterapico, por meio de citologia esfoliativa (papanicolau e PAS) e esfregaços corados pelo Gram. Comprovou-se um aumento de leveduras durante o tratamento, predominando as formas filamentosas consideradas mais patogênicas. Podem-se detectar áreas bem definidas de candidose, quer do tipo atrófica ou pseudomembranosa, além de áreas de radiomucosite, embora a associaçäo de lesöes brancas e eritematosas predominassem levando a um mascaramento do quadro clínico presente. Aumento significativo de sintomas desde a xerostomia, entre outros, foi observado durante o tratamento.

Lesiones esofágicas causadas por el virus del papiloma humano (VPH) / Esophageal injuries due to human papillomaviruses (HP)

Describimos 22 enfermos con lesiones histopatológicas en las biopsias esofágicas compatibles con infección por VPH. Los síntomas por los cuales consultaron los pacientes, no tuvieron nada de característico: pirosis con o sin odinofagia, otras veces era epigastralgia de carácter irregular e intermitente, disfalgia en dos casos con carcinoma y un caso asintomático. No hubo ninguna predilección para ningún sexo y la edad promedio del gupo fue de cuarenta años. La endoscopia digestiva superior mostró los siguientes hallazgos: a) Ulceras en el tercio medio, la mayoría menores de 10 mm, redondeadas u ovaladas, de profundidad moderada, separadas por mucosa sana aparentemente, con bordes bien delimitados e hiperémicos, fondo blanquecino y liso; b) En cinco enfermos hubo lesiones blanquecinas elevadas, también situadas en el tercio medio del órgano, múltiples, separadas por mucosa sin lesiones, no cubiertas con membranas fáciles de separar. Un paciente presentó dos papilomas; c) En dos casos hubo carcinoma epidermoide a 25 y 28 cm de la arcada dental, vegetante y estenosante. Las biopsias tomadas en la periferia del tumor, demostraron lesiones compatibles con VPH. La posible relación de las lesiones descritas aparentemente ceden sin tratamiento o con medicamento no específico, aunque algunos casos de nuestra serie han respondido en forma empírica a drogas antivirales

Atividade de anti-sépticos contra leveduras isoladas da cavidade oral de pacientes com cancer de boca antes e durante a radioterapia / Antiseptics activity against yeasts isolated of the oral cavity from patients with mouth neoplasm before and during radiotherapy

Foi estudada, "in vitro", a atividade antifúngica de cinco anti-sépticos contra leveduras isoladas antes e durante a radioterapia de pacientes de carcinoma epidermóide de boca. A concentraçäo inibitória mínima do triclosan variou de < ou = 0,07 g/L a 20 > g/L, conforme a cepa estudada, enquanto que a dos outros anti-sépticos foi invariavelemente < ou = 0,07 g/L. O hexaclorofeno e o timerosal foram fungicidas também em concentraçöes < ou = 0,7 g/L para todas as leveduras avaliadas, e o cloreto de cetilpiridino o foi a 0;31 g/L. Em concentraçöes de até 20g/L, o triclosan näo demonstrou atividade fungicida contra a maior parte das leveduras, e a violeta de genciana näo foi letal somente para duas cepas de C. albicans isoladas de pacientes que se encontravam sob radioterapia

Association of Candida with carcinoma of esophagus.

Twenty-five patients with carcinoma of the esophagus (group I) and 25 patients suffering from non-ulcer dyspepsia with normal endoscopy (group II) were studied to know the incidence of isolation of Candida from their esophagus. Endoscopic brushings were taken from the esophagus in both groups and studied by smear examination and culture. Fungal organisms could be detected in 75% of cases of group I and 32% of cases of group II by culture techniques, and 45.8% and 12% respectively by smear examination. The difference was statistically significant (p less than 0.05) for both the techniques. Candida albicans was the commonest species isolated. No correlation was found between Candida agglutination titres and density of Candida growth on culture. We conclude that an association exists between carcinoma esophagus and the occurrence of Candida in the esophagus.

Laryngeal squamous cell carcinoma: demonstration of human papilloma virus [HPV] antigen and possible associated condylomatous changes of the epithelium

Twenty biopsies of laryngeal squamous cell carcinoma were subjected to histological examination in this study after being fixed in 10% buffered formalin, embedded in paraffin and stained with both haematoxylin and Eosin [H and E] and immunoprexidase stain. 13 cases showed papillomatous changes while 7 cases showed flat type condylomatous changes. 50% of the total cases showed positive reactivity with immunoperoxidase stain. 53.8% of the tumours with the papillomatous changes and 42.9% of those with flat type changes showed positive reactivity for HPV antigen particles