ABSTRACT
Polyynes, such as facarindiol (FAD) and oplopandiol (OPD), are responsible for anticancer activities of Oplopanax elatus (O. elatus). A novel approach to pharmacokinetics determination of the two natural polyynes in rats was developed and validated using a liquid chromatography-electrospray ionization-mass spectrometry (LC-MS) method. Biosamples were prepared by liquid-liquid extraction using ethyl acetate/n-hexane (V : V = 9 : 1) and the analytes were eluted on an Agilent ZORBAX Eclipse Plus C18 threaded column (4.6 mm × 50 mm, 1.8 μm) with the mobile phase of acetonitrile-0.1% aqueous formic acid at a flow-rate of 0.5 mL·min(-1) within a total run time of 11 min. All analytes were simultaneously monitored in a single-quadrupole mass spectrometer in the selected ion monitoring (SIM) mode using electrospray source in positive mode. The method was demonstrated to be rapid, sensitive, and reliable, and it was successfully applied to the pharmacokinetic studies of the two polyynes in rat plasma after oral administration of polyynes extract of O. elatus.
Subject(s)
Animals , Male , Rats , Administration, Oral , Chromatography, High Pressure Liquid , Methods , Diynes , Pharmacokinetics , Drugs, Chinese Herbal , Pharmacokinetics , Fatty Alcohols , Pharmacokinetics , Naphthols , Pharmacokinetics , Oplopanax , Chemistry , Polyynes , Pharmacokinetics , Rats, Sprague-Dawley , Spectrometry, Mass, Electrospray Ionization , MethodsABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC method for simultaneous determination of panaxynol and panaxydol from the fibrous root of Panax ginseng.</p><p><b>METHOD</b>The analysis was performed on Elite C18 column (4.6 mm x 150 mm, 5 microm) with mobile phase gradient of CH3CN-water at a flow rate of 1.0 mL x min(-1). The detection wavelength was 230 nm, and the detection temperature was ambient.</p><p><b>RESULT</b>The linear range were 0.70-3.50 microg (r = 0.9995) for panaxynol, and 0.64-3.20 microg (r = 0.9999) for panaxydol. The average recoveries were 99.1% (RSD 1.7%) and 99.3% (RSD 1.2%), respectively.</p><p><b>CONCLUSION</b>The HPLC method is simple, rapid and reproducible, which can be used for the quality control of the fibrous root of P. ginseng.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Diynes , Drugs, Chinese Herbal , Fatty Alcohols , Panax , Chemistry , Plant Roots , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of panaxynol (PAN) on delayed type hypersensitivity and possible mechanism.</p><p><b>METHOD</b>Allergic contact dermatitis (ACD) was induced by DNCB as a delayed type hypersensitivity (DTH) model to observe effect of PAN on auricle inflammation including pathological injury. Proliferation of T lymphocytes was induced by ConA and measured by MTf method. IFN-gamma secretion of splenocyte induced by ConA was detected by ELISA.</p><p><b>RESULT</b>The swelling degree of auricle and pathological injury in ACD mice was reduced significantly by treated with PAN in induction phase. Proliferation of T lymphocytes induced by ConA in vitro was inhibited significantly by PAN, By contrast, no detectable effect was observed in resting splenocyte. IFN-y induced by ConA in splenocytes was inhibited markedly by PAN from 10 micromol x L(-1) and from 6 h.</p><p><b>CONCLUSION</b>The results showed that DTH was inhibited by PAN mainly in induction phase and this effect may be related with the inhibition on T lymphocytes proliferation and secretion of IFN-gamma.</p>