ABSTRACT
Giardia lamblia infection has been shown to modify the glycosylation process of microvillus membranes in mice intestine. Sialic acid content of the membranes was enhanced 3-fold but there was no change in fucose content of infected animals compared to the controls. The binding of 125I-wheat germ agglutinin was augmented and that of Ulex Europeus agglutinin was unaltered to infected membranes. The binding of 125I-peanut agglutinin to brush borders was however, significantly reduced in Giardia infected mice. Kinetic analysis revealed that the observed binding of peanut agglutinin to the membrane was associated with reduced number of the lectin reactive sites (control = 649 and infected 380 nmole/protein) with no change in the affinity constant (Ka = 95.7 nmole/ml) in Giardia infected mice intestine.
Subject(s)
Animals , Female , Giardiasis/metabolism , Glycosylation , Intestines/metabolism , Lectins/metabolism , Male , Mice , Microvilli/metabolism , Sialic Acids/metabolismABSTRACT
The absorption of 125I-labelled bovine serum albumin, gamma-globulin and alpha-lactalbumin was considerably enhanced in G. lamblia infected Swiss mice intestine compared to uninfected controls. The binding of 125I-proteins to brush border membrane was however, significantly (P less than 0.01) low in infected animals. Kinetic studies with gamma-globulin binding to brush borders revealed a decrease in the number of binding sites in infected animals (1.52) compared to controls (2.86 micrograms/mg protein) with no change in the affinity constant (47.60 micrograms/ml) under these conditions. These findings suggest that G. lamblia infection in mice leads to enhanced macromolecular absorption which seems unrelated to the binding of proteins to epithelial cell surface.
Subject(s)
Animals , Disease Models, Animal , Female , Giardiasis/metabolism , Intestinal Absorption , Lactalbumin/metabolism , Male , Mice , Microvilli/metabolism , Serum Albumin, Bovine/metabolism , gamma-Globulins/metabolismABSTRACT
Con el propósito de estudiar si la G. lamblia se acompaña en el intestino de una gran población de bacterias productoras de indol, se investigó la concentración de sulfato de indoxil en la orina, antes y después de erradicar este parásito. Los resultados plantean, indirectamente, que la giardiasis se asocia a una proliferación de enterobacterias que sobrepasa en magnitud la que ordinariamente existe en el intestino
Subject(s)
Child, Preschool , Child , Humans , Male , Female , Giardia/physiology , Indoles/urine , Intestines/microbiology , Sulfates/urine , Enterobacteriaceae/growth & development , Enterobacteriaceae/metabolism , Giardiasis/metabolism , Giardiasis/physiopathology , Giardia/metabolism , Indoles/biosynthesis , Intestinal AbsorptionABSTRACT
La prueba de absorción a la lactosa se estudió en 35 niños con Giardia Lamblia. En 24/35, existió absorción deficiente de lactosa. No existió ninguna asociación significativa entre la absorción deficiente de lactosa y la edad de los pacientes así como en relación al peso y diarrea. En 23/35 el motivo de consulta fue diarrea crónica y/o prolongada; en 11/35 dolor abdominal y en 1/35 talla baja. Existió una asociación estadísticamente significativa entre absorción deficiente de lactosa y dolor abdominal, prueba de X**2, P<0.05. Es posible que la absorción deficiente de lactosa sea un factor más en la génesis del dolor abdominal en niños con Giardia Lamblia. No es posible precisar si esta absorción deficiente de lactosa pueda ser debida más a aspectos étnicos