ABSTRACT
OBJECTIVE: To assess the role of transforming growth factor-β1 (TGF-β1) in congenital ureteropelvic junction obstruction at diagnosis and during postoperative follow-up. MATERIAL AND METHODS: We conducted a case-control study including 19 patients with a mean age of 6.7 years and 19 matched controls. All patients presented negative voiding cystourethrography, obstructive diuretic renogram and underwent dismembered pyeloplasty. Urinary TGF-β1 and other markers were measured pre-, intra- and postoperatively. RESULTS: The mean bladder urine TGF-β1 concentration in obstructed patients prior to pyeloplasty was higher than in controls (92.5 pg/mL ± 16.8 vs. 35.8 pg/mL ± 16.2; p = 0.0001). The mean renal pelvic urine TGF-β1 concentration in the hydronephrotic kidney was higher than in the preoperative bladder urine sample (122.3 pg/mL ± 43.9 vs. 92.5 pg/mL ± 16.8; p = 0.036). Postoperative mean TGF-β1 concentration was significantly lower than preoperative TGF-β1 (48.7 pg/mL ± 13.1 vs. 92.5 pg/mL ± 16.8; p = 0.0001). CONCLUSION: TGF-β1 is a cytokine leading to renal fibrosis. The measurement of urinary TGF-β1 could become a useful tool for the diagnosis of obstructive hydronephrosis and the evaluation of the parenchyma function status, pre and postoperatively.
Subject(s)
Child , Female , Humans , Male , Hydronephrosis/diagnosis , Transforming Growth Factor beta1/urine , Ureteral Obstruction/diagnosis , Biomarkers/urine , Case-Control Studies , Follow-Up Studies , Hydronephrosis/urine , Kidney Pelvis , Perioperative Period , Sensitivity and Specificity , Treatment Outcome , Ureteral Obstruction/congenital , Ureteral Obstruction/surgery , Ureteral Obstruction/urine , Urinary Bladder/metabolism , Vesico-Ureteral Reflux/diagnosisABSTRACT
OBJECTIVE: Hydronephrosis leads to deterioration of renal function. As urinary N-acetyl-beta-D-glucosaminidase (U-NAG) activity is considered a sensitive marker of renal tubular impairment, our aim was to measure U-NAG in children with hydronephrosis and to look for a relationship among selected clinical parameters. MATERIALS AND METHODS: We studied 31 children (22 boys and 9 girls, mean age 2.3 ± 2.5 years) with hydronephrosis grade 1-4 that had U-NAG/creatinine ratio (U-NAG/Cr) measured. RESULTS: The U-NAG/Cr was significantly higher in patients with hydronephrosis compared to reference data (p = 0.002). There was no difference in U-NAG/Cr between children with unilateral and bilateral hydronephrosis (p = 0.51). There was no significant difference in U-NAG/Cr between children with grades 1-3 (pooled data) and grade 4, respectively (p = 0.89). There was no correlation between U-NAG/Cr and the grade of hydronephrosis (r = 0.01). CONCLUSIONS: U-NAG/Cr is increased in children with hydronephrosis grade 1-4, and there is no relationship with the grade of hydronephrosis. U-NAG is a useful marker of renal tubular dysfunction, however its relationship with the degree of kidney damage in patients with hydronephrosis should be considered as doubtful.
Subject(s)
Humans , Male , Female , Child, Preschool , Acetylglucosaminidase/urine , Hydronephrosis/urine , Kidney Tubules/physiopathology , Biomarkers/urine , Hydronephrosis/enzymology , Hydronephrosis/physiopathology , Severity of Illness IndexABSTRACT
Se entiende por hidronefrosis la dilatación del sistema colector renal y por obstrucción la restricción al flujo urinario que de no ser tratado causaría deterioro renal progresivo. El objetivo es mostrar la experiencia de los casos de pieloectasia atendidos en la unidad de nefrología pediátrica del Hospital Materno Infantil de la Caja Nacional de Salud.