study on the relationship between thyroid hormones and adenosine deaminase enzyme activity in patients with auto- immune hyperthyroid disease

Adenosine deaminase ADA catalyzes the deamination of adenosine in purine metabolism. Adenosine deaminase deficiency is an inborn error resulting in immunodeficiency condition. Clinical interest in this enzyme has been revived by the discovery of a syndrome of severe humoral and cellular immunodeficiency associated with deficiency of ADA in somatic erythrocyte cell. The aim of present study is conducted to find the relationship between human hyperthyroid activities and immune etiology in graves disease. Forty individuals with hyperthyroidism autoimmune state were included in this study in comparison with forty normal persons. Thyroid hormones levels were estimated by HPLC and serum ADA specific activity was estimated using spctrophotometric method at 265 nm. Serum protein value was determined by Bradford method. Serum ADA activity was measured for 40 hyperthyroid patients as well as 40 normal individuals. The mean activity of ADA in patients was higher than in the controls. A significant increase in T3/T4 ratio and level of TSH were noticed. A positive correlation between thyroid level and ADA enzyme activity was noticed

Validity of serum CK activity in the diagnosis of thyroid dysfunction

This study assessed the pattern of changes in serum creatine kinase [CK] activity in [100] patients with a variety of clinical manifestations of either increased or decreased thyroid function and evaluated the validity of such changes in the diagnosis of both conditions They were biochemically assessed for thyroid function and compared with a control group of 67 apparently healthy subjects According to thyroid function tests, the subjects were classified into 3 groups Group 1 Hypothyroid patients [n=50], which was divided into 2 subgroups Group A[Overt hypothyroidism] It included 43 patients with TT4 60 nmol/l. The second group [Group 2] included 50 hyperthyroid patients, while [Group 3] was composed of 67 euthyroid subjects. Serum CK activity was noticeably elevated in hypothyroid patients compared to both normal and hyperthyroid subjects [mean +SD was 204.10 + 97.72 Vs 79.97+ 26.35 and 79.36 + 26.88 u/L respectively]. There was no significant difference in the mean serum CK activity between group A and B [Z =87, P>0.05] A significant positive correlation was revealed between serum TSH and CK in hypothyroid patients [Group I] where [r=0.35, P<0.01], while there was no such significant association in either group A or B [p>O.O5],and thus did the hyperthyroid patients [r= -0.22, P>0.05]. For patients in group1, the sensitivity, specificity, positive predictive value [PPV], negative predictive value [NPV], accuracy rate, positive likelihood ratio [PLR] and negative likelihood ratio [NLR] of elevated serum CK activity as a diagnostic test were 74%, 95.5%, 92.5%, 87%, 86%, 16.40, 0.27 respectively. However, they were 0%, 95.5%, 0%, 56%, 5.4%, 0 and 0.96 respectively for the diagnosis of hyperthyroidism. In conclusion high serum CK activity is a fairly sensitive and highly specific test for the diagnosis of hypothyroidism with a high PPV value mainly for overt [uncompensated] cases. This study indicates that the determination of CK might be a useful screening tool for hypothyroidism

Dorsolateral prostatic phosphomonoesterases and adenosine triphosphatases in hypo- and hyperthyroid rats.

Specific activities of phosphomonoesterases (acid and alkaline phosphatases) and adenosine triphosphatases (Mg2+, Ca2+ and Na+/K+ dependent ATPases) of dorsolateral prostate were studied in albino rats, under altered thyroid hormone status. Thyroidectomy induced hypothyroidism and thyroxine administered hyperthyroidism (25 micrograms/100 g body wt/day for 60 days, im) showed no impact on the activity of acid phosphatase. Three fold decrease in the activity of alkaline phosphatase was observed in hyperthyroid group. Ca2+ and Mg2+ dependent ATPases were significantly decreased in hypo- and hyperthyroid status whereas Na+/K+ ATPase was decreased in hyperthyroidism and showed an opposite trend in hypothyroid group.

Specific activities of seminal vesicular phosphomonoesterases and Mg2+-,Ca2+- and Na+/K(+)-adenosine triphosphatases in hypo- and hyperthyroid albino rats.

Hypothyroidism (surgical thyroidectomy) inhibited the activities of acid phosphatase and Mg(2+)-ATPase in seminal vesicular tissue and fluid and that of Ca(2+)- and Na+/K(+)-ATPases in fluid alone, and T4 supplementation restored normalcy in all, except acid phosphatase. Hyperthyroidism (T4 25 micrograms/100g body weight/day for 60 days, im) enhanced the activities of alkaline phosphatase and ATPases in seminal vesicular tissue and fluid, and decreased acid phosphatase activity in tissue alone. Withdrawal of T4 treatment from hyperthyroid rats (after 30 days) augmented the activity of ATPases in tissue and impaired the same in fluid, while phosphomonoesterases remained at hyperthyroid level. The results suggest specific responses of various seminal vesicular phosphatases to altered thyroid hormone status. Modification in the specific threshold of androgen/estrogen action on different phosphatases in seminal vesicles appears to be the plausible mechanism underlying these changes in hypo- and hyperthyroid conditions.

Ventral prostatic phosphomonoesterases and adenosine triphosphatases in hypo- and hyperthyroid albino rats.

Specific activities of prostatic phosphomonoesterases (acid and alkaline phosphatases) and adenosine triphosphatases (Mg2+, Ca2+ and Na+/K+ dependent ATPases) were studied in albino rats, under altered thyroid hormone status. Thyroidectomy induced hypothyroidism decreased the specific activities of acid and alkaline phosphatases, Na+/K+ and Ca2+ dependent ATPases in ventral prostate. Hyperthyroidism (25 micrograms thyroxine/100g body weight/day for 60 days, im) enhanced the activities of acid phosphatase and Na+/K+ dependent ATPase, while Ca2+ dependent ATPase decreased. The altered thyroid status had no effect on the activity of ventral prostatic Mg2+ dependent ATPase. The data obtained in the present study showed differential and specific responses of various ventral prostatic phosphatases to the hypo or hyperthyroid status. The study also shows the necessity of an optimum level of thyroid hormones to maintain the normal activities of these enzymes and their secretory function in ventral prostate.