ABSTRACT
Human brucellosis is an occupational disease affecting workers in slaughterhouses, butcher shops and the milk and dairy product industry as well as individuals who work in clinical or research laboratories. We report the first outbreak of a Brucella abortus infection in a Brazilian laboratory and compare the data obtained with reports available in the literature. Exposure was a result of damage to a biological safety cabinet and failure of the unidirectional airflow ventilation system. An epidemiological investigation identified 3 seroconverted individuals, 1 of whom had clinical manifestations and laboratory results compatible with infection at the time of exposure (n=11; attack rate=9.1%).
Subject(s)
Adult , Female , Humans , Male , Young Adult , Accidents, Occupational , Brucella abortus/immunology , Brucellosis/epidemiology , Laboratory Infection/epidemiology , Antibodies, Bacterial/blood , Brazil/epidemiology , Brucellosis/diagnosis , Brucellosis/immunology , Disease Outbreaks , Laboratory Infection/diagnosis , Laboratory Infection/immunology , Medical Laboratory PersonnelABSTRACT
Strains of Salmonella typhi implicated in two separate cases of laboratory acquired infection from patients and the medical laboratory technologists who processed the patients' samples were analysed by pulsed-field gel electrophoresis. Although all four isolates were of bacteriophage type E1, PFGE was able to demonstrate that the strains responsible for the two laboratory acquired cases were not genetically related. The PFGE patterns of the isolates from the MLTs were found to be identical to those of the corresponding patients after digestion with restriction enzyme AvrII. This provided genetic as well as epidemiological evidence for the source of the laboratory acquired infections.
Subject(s)
Bacteriophage Typing , DNA Fingerprinting , Electrophoresis, Gel, Pulsed-Field , Endemic Diseases , Humans , Laboratory Infection/diagnosis , Malaysia , Salmonella typhi/genetics , Typhoid Fever/diagnosisABSTRACT
Sao descritos os achados clinico-laboratoriais da infeccao acidental pelo virus SP H 114202 (Arenavirus, familia Arenaviridae), um virus novo causador de febre hemorragica humana. O paciente, tecnico de laboratorio, apresentou quadro febril por 13 dias. A doenca cursou com febre elevada (39ºC) diaria, cefaleia, calefrios e mialgias por 8 dias. A partir do 3§ dia surgiram nauseas, vomitos alimentares e anorexia e no 10§ dia, epigastralgia, diarreia e gengivorragia....