ABSTRACT
Astrocytes are the most abundant cells in the central nervous system that play roles in maintaining the blood-brain-barrier and in neural injury, including cerebral malaria, a severe complication of Plasmodium falciparum infection. Prostaglandin (PG) D2 is abundantly produced in the brain and regulates the sleep response. Moreover, PGD2 is a potential factor derived from P. falciparum within erythrocytes. Heme oxygenase-1 (HO-1) is catalyzing enzyme in heme breakdown process to release iron, carbon monoxide, and biliverdin/bilirubin, and may influence iron supply to the P. falciparum parasites. Here, we showed that treatment of a human astrocyte cell line, CCF-STTG1, with PGD2 significantly increased the expression levels of HO-1 mRNA by RT-PCR. Western blot analysis showed that PGD2 treatment increased the level of HO-1 protein, in a dose- and time-dependent manner. Thus, PGD2 may be involved in the pathogenesis of cerebral malaria by inducing HO-1 expression in malaria patients.
Subject(s)
Animals , Humans , Astrocytes/enzymology , Blotting, Western , Cell Line , Gene Expression Profiling , Heme Oxygenase-1/biosynthesis , Malaria, Cerebral/pathology , Malaria, Falciparum/complications , Plasmodium falciparum/pathogenicity , Prostaglandins/metabolism , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Malaria is one of the most common diseases in tropical countries. In our country, malaria is endemic in some parts of south and east. Cerebral malaria is usually a diffuse symmetric encephalopathy with focal signs being unusual. We present a two-year old girl with fever and seizure while undergoing treatment for malaria. Imaging disclosed acute subdural empyema. Investigations revealed anemia, thrombocytopenia and positive peripheral blood smear for vivax malaria. Treatment involved surgical evacuation of the subdural empyema, oral chloroquin and antibiotics. This is the third case report of spontaneous subdural empyema in complicated malaria and highlights a rare but surgically manageable complication
Subject(s)
Humans , Female , Malaria, Cerebral/diagnosis , Malaria, Cerebral/pathology , Malaria/complications , Malaria, Cerebral/therapy , Empyema, Subdural/diagnostic imaging , Empyema, Subdural/surgery , Plasmodium vivaxABSTRACT
Immune responses to malaria infections are characterized by strong T and B cell activation, which, in addition of potentially causing immunopathology, are of poor efficacy against the infection. It is possible that the thymus is involved in the origin of immunopathological reactions and a target during malaria infections. This work was developed in an attempt to further clarify these points. We studied the sequential changes in the thymus of CBA mice infected with Plasmodium berghei ANKA, a model in which 60-90 percent of the infected animals develop cerebral malaria. During the acute phase of infection, different degrees of thymocyte apoptosis were recorded: (1) starry-sky pattern of diffuse apoptosis with maintenance of cortical-medullary structure; (2) intense apoptosis with cortical atrophy, with absence of large cells; (3) severe cortical thymocyte depletion, resulting in cortical-medullary inversion. In the latter, only residual clusters of small thymocytes were observed within the framework of epithelial cells. The intensity of thymus alterations could not be associated with the degree of parasitemia, the expression of clinical signs of cerebral malaria or intensity of brain lesions. The implications of these events for malaria immunity and pathology are discussed.