Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos

The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.

Mastocitoma solitario: presentación de un caso / Solitary mastocytoma: report of a case

Se presenta el caso de un lactante menor con tumoración cutánea benigna localizada en el aspecto submandibular izquierdo de la cabeza compatible con mastocitoma cutáneo solitario. Esta rara lesión tumoral es parte del grupo heterogéneo de las mastocitosis, conjunto de enfermedades que resulta de la infiltración anómala de mastocitos en diferentes tejidos corporales, afectando particularmente la piel.La variedad más común de mastocitosis en niños es la urticaria pigmentosa, seguida del mastocitoma cutáneo que contabiliza entre el 10 y 15% de todos los casos. Suele situarse en tronco o extremidades, aunque como en este paciente puede localizarse en cabeza uotros sitios corporales. Es de aparición precoz durante la primera infancia o alguna vez congénito; por lo regular no hay participación de órganos internos o situaciones potencialmente letales. Su curso es crónico con remisiones parciales y exacerbaciones sintomáticasmolestas como fenómenos de urticación y desarrollo de ampollas frente a estímulos externos o internos que preocupan a los familiares cercanos. Su reconocimiento clínico precoz y la pronta instauración terapéutica con esteroides locales, antihistamínicos orales u otras medidas pertinentes, aceleran el tiempo de resolución casi siempre espontánea hacia los 2 años de vida; es importante la debida explicación a los padres

This is a report the case of an infant with benign cutaneous tumor located in the left submandibular aspect of the head support solitary cutaneous mastocytoma. This rare tumor lesion is part of the heterogeneous group of mastocytosis, group of diseases resulting from abnormal infiltration of mast cells in various body tissues, particularly affecting the skin. The most common variety of mastocytosis inchildren is urticaria pigmentosa, followed by cutaneous mastocytoma counted between 10 and 15% of all cases. Usually located on the trunk or extremities, although as in this patient may be located in the head or other body sites. Is early onset in infancy or congenital ever, usually no involvement of internal organs or life-threatening situations. Its course is chronic symptomatic partial remissions and exacerbations Urtication annoying as phenomena and development of blisters against external or internal stimuli which trouble close relatives. Its early clinical recognition and prompt establishment local steroid therapy, oral antihistamines or other relevant measures speed time to spontaneous resolution usually 2 years of life, is important due explanation to parents.

Mastocitoma solitario cutáneo / Solitary cutaneous mastocytoma

El mastocitoma solitario es una lesión tumoral benigna de mastocitos, que se presenta de preferencia en la edad pediátrica. Puede ubicarse en cualquier órgano, pero es más común encontrarlo en la piel. Se caracteriza por la presencia del signo de Darier. Los síntomas pueden ser muy variados y se correlacionan con la liberación de mediadores mastocíticos. Es importante evaluar la existencia de una mastocitosis sistémica asociada, según los criterios recomendados por la OMS. En general la evolución es benigna; sin embargo, existe una gran variedad de elementos terapéuticos disponibles. A continuación presentamos el caso de un lactante mayor que consulta por una lesión marrón-amarillenta en dorso que sufre cambios en relación a la fricción; el estudio histológico concluye el diagnóstico de mastocitoma. Se realiza una revisión actualizada de la literatura, con especial énfasis en las manifestaciones clínicas y opciones terapéuticas.

Solitary mastocytoma is benign tumour of mast cells, which occurs predominantly in children. It can affect any organ, but is most commonly found in the skin. It is characterized by the presence of the Darier’s sign. Symptoms can be variable and are correlated with the release of mast cell mediators. It is important to assess the existence of an associated systemic mastocytosis, according to the criteria recommended by the WHO. In general, evolution is benign, however there is a great variety of therapeutic possibilities available. We present the case of an infant who complains of a brown-yellowish lesion on the back that undergoes changes in relation to rubbing. Histological studies revealed mastocytosis. We present an updated review of the literature with special emphasis on the clinical manifestations and therapeutic options.